{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/121357"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/121357","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Roles of non-Cas nucleases in Type III-A CRISPR-Cas immunity","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01","abstract_has_math":false,"creators":["Chou Zheng, Lucy"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Hatoum-Aslan, Asma","Kuzminov, Andrei","Mera, Paola E.","Nair, Satish K."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-08","date_published":"2023-08","updated_at":"2026-07-22T22:24:57Z","subjects":["Crispr-cas10","Type Iii-a Crispr-cas","Cas10-csm","Crispr-cas","Degradosome, Staphylococci","Staphylococcus Epidermidis","Pnpase","Rnase R","Rnase J2","Nhi","Anti-phage Defense."],"languages":["en","eng"],"rights":["Copyright 2023 Lucy Chou Zheng"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/121357","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hatoum-Aslan, Asma","Kuzminov, Andrei","Mera, Paola E.","Nair, Satish K."]},{"key":"dc:creator","label":"Author","values":["Chou Zheng, Lucy"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-08","2023-07-13"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Crispr-cas10","Type Iii-a Crispr-cas","Cas10-csm","Crispr-cas","Degradosome, Staphylococci","Staphylococcus Epidermidis","Pnpase","Rnase R","Rnase J2","Nhi","Anti-phage Defense."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Lucy Chou Zheng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/121357"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01","The student, Lucy Chou Zheng, accepted the attached license on 2023-07-12 at 01:18.","The student, Lucy Chou Zheng, submitted this Dissertation for approval on 2023-07-12 at 02:21.","This Dissertation was approved for publication on 2023-07-13 at 11:25.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19642 on 2023-12-04 at 17:18:35","The overuse of antibiotics has led to a new wave of resistant bacterial pathogens insensitive to current antibiotics. Staphylococcus species are ubiquitous residents of human skin that can acquire and disseminate antibiotic resistance genes (ARGs) through horizontal gene transfer (HGT). Therefore, it is essential to understand cellular processes that naturally block HGT and prevent the transfer of ARGs. One such process is mediated by Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPRs) and their CRISPR-associated (Cas) proteins. CRISPR-Cas systems are a class of prokaryotic immune systems that use small CRISPR RNAs (crRNAs) as guides and Cas nucleases as weapons to detect and destroy mobile genetic elements, including plasmids and bacteriophages. The work in this thesis investigates the mechanism of CRISPR-Cas immunity using S. epidermidis RP62a, a commensal opportunistic pathogen, as the model organism. This research investigates and proves the central hypothesis that the Type III-A CRISPR-Cas system collaborates with housekeeping nucleases to mount a successful defense against genetic invaders."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Roles of non-Cas nucleases in Type III-A CRISPR-Cas immunity"]}]}],"canonical_facts":{"dc:contributor":["Hatoum-Aslan, Asma","Kuzminov, Andrei","Mera, Paola E.","Nair, Satish K."],"dc:creator":["Chou Zheng, Lucy"],"dc:date":["2023-08","2023-07-13"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01","The student, Lucy Chou Zheng, accepted the attached license on 2023-07-12 at 01:18.","The student, Lucy Chou Zheng, submitted this Dissertation for approval on 2023-07-12 at 02:21.","This Dissertation was approved for publication on 2023-07-13 at 11:25.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19642 on 2023-12-04 at 17:18:35","The overuse of antibiotics has led to a new wave of resistant bacterial pathogens insensitive to current antibiotics. Staphylococcus species are ubiquitous residents of human skin that can acquire and disseminate antibiotic resistance genes (ARGs) through horizontal gene transfer (HGT). Therefore, it is essential to understand cellular processes that naturally block HGT and prevent the transfer of ARGs. One such process is mediated by Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPRs) and their CRISPR-associated (Cas) proteins. CRISPR-Cas systems are a class of prokaryotic immune systems that use small CRISPR RNAs (crRNAs) as guides and Cas nucleases as weapons to detect and destroy mobile genetic elements, including plasmids and bacteriophages. The work in this thesis investigates the mechanism of CRISPR-Cas immunity using S. epidermidis RP62a, a commensal opportunistic pathogen, as the model organism. This research investigates and proves the central hypothesis that the Type III-A CRISPR-Cas system collaborates with housekeeping nucleases to mount a successful defense against genetic invaders."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/121357"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Lucy Chou Zheng"],"dc:subject":["Crispr-cas10","Type Iii-a Crispr-cas","Cas10-csm","Crispr-cas","Degradosome, Staphylococci","Staphylococcus Epidermidis","Pnpase","Rnase R","Rnase J2","Nhi","Anti-phage Defense."],"dc:title":["Roles of non-Cas nucleases in Type III-A CRISPR-Cas immunity"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:57Z"}