{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/121301"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/121301","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Microenvironmental modification and analysis of 3D cell culture using microgels","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01","abstract_has_math":false,"creators":["Ryoo, Hyeon"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Underhill, Gregory H","Kong, Hyunjoon","Perez-Pinera, Pablo","Wang, Ning"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-08","date_published":"2023-08","updated_at":"2026-07-22T22:24:57Z","subjects":["Microgels","3d Cell Culture","Granular Hydrogels","High Throughput Screening","Sensor","Il-6","Ecm"],"languages":["en","eng"],"rights":["Copyright 2023 Hyeon Ryoo"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/121301","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Underhill, Gregory H","Kong, Hyunjoon","Perez-Pinera, Pablo","Wang, Ning"]},{"key":"dc:creator","label":"Author","values":["Ryoo, Hyeon"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-08","2023-06-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microgels","3d Cell Culture","Granular Hydrogels","High Throughput Screening","Sensor","Il-6","Ecm"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Hyeon Ryoo"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/121301"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01","The student, Hyeon Ryoo, accepted the attached license on 2023-06-07 at 22:40.","The student, Hyeon Ryoo, submitted this Dissertation for approval on 2023-06-07 at 22:44.","This Dissertation was approved for publication on 2023-06-12 at 09:14.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19408 on 2023-12-04 at 17:17:52","The complex, heterotypic, and dynamic microscale surroundings of cells, termed the cellular microenvironment, determines cell behavior. As such, efforts to characterize and modify cellular microenvironments are abound within bioengineering and related fields, with an increasing amount of research efforts being translated from 2D in vitro culture to 3D culture, due to the potential for increased in vivo relevance within 3D systems. Hydrogels are a particularly popular material for 3D cell culture based on their resemblance to native tissue. For example, PEG hydrogels are commonly used for their high modularity and bioinert nature, but they can exhibit some limitations due to nanoporosity. PEG microgels are able to retain the modularity of bulk PEG hydrogels while adding microporosity, injectability and heterogeneity to the system. By using PEG-acrylate microgels of 5.84 µm, we were able to create a miniaturized sandwich ELISA system that could detect gradients of 2.21- 21.86 ng/mL of IL-6 and 92.56 – 2259.08 ng/mL of MMP2 in 3D space. By using PEG-norbornene microgels of ~9.74 µm, varied stiffnesses of 5.72 and 24.44 kPa and tagged with fibronectin, collagen I, III or IV, we enabled the modulation of hepatic stellate cell activation. Further, by automating the distribution of these protein-tagged microgels, we created heterogeneous scaffolds to serve as high throughput ECM screening platforms for the quantification of hepatic stellate cell matrix remodeling and metabolic activity. The high throughput ECM screening platform could be expanded to other areas to identify ECM conditions that can lead to optimal directed differentiation, cell phenotype or drug efficacy. We believe these cellular microenvironment characterization and modification techniques can expand upon existing 3D cell culture platforms and allow for new insights into 3D cellular behavior."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Microenvironmental modification and analysis of 3D cell culture using microgels"]}]}],"canonical_facts":{"dc:contributor":["Underhill, Gregory H","Kong, Hyunjoon","Perez-Pinera, Pablo","Wang, Ning"],"dc:creator":["Ryoo, Hyeon"],"dc:date":["2023-08","2023-06-12"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01","The student, Hyeon Ryoo, accepted the attached license on 2023-06-07 at 22:40.","The student, Hyeon Ryoo, submitted this Dissertation for approval on 2023-06-07 at 22:44.","This Dissertation was approved for publication on 2023-06-12 at 09:14.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19408 on 2023-12-04 at 17:17:52","The complex, heterotypic, and dynamic microscale surroundings of cells, termed the cellular microenvironment, determines cell behavior. As such, efforts to characterize and modify cellular microenvironments are abound within bioengineering and related fields, with an increasing amount of research efforts being translated from 2D in vitro culture to 3D culture, due to the potential for increased in vivo relevance within 3D systems. Hydrogels are a particularly popular material for 3D cell culture based on their resemblance to native tissue. For example, PEG hydrogels are commonly used for their high modularity and bioinert nature, but they can exhibit some limitations due to nanoporosity. PEG microgels are able to retain the modularity of bulk PEG hydrogels while adding microporosity, injectability and heterogeneity to the system. By using PEG-acrylate microgels of 5.84 µm, we were able to create a miniaturized sandwich ELISA system that could detect gradients of 2.21- 21.86 ng/mL of IL-6 and 92.56 – 2259.08 ng/mL of MMP2 in 3D space. By using PEG-norbornene microgels of ~9.74 µm, varied stiffnesses of 5.72 and 24.44 kPa and tagged with fibronectin, collagen I, III or IV, we enabled the modulation of hepatic stellate cell activation. Further, by automating the distribution of these protein-tagged microgels, we created heterogeneous scaffolds to serve as high throughput ECM screening platforms for the quantification of hepatic stellate cell matrix remodeling and metabolic activity. The high throughput ECM screening platform could be expanded to other areas to identify ECM conditions that can lead to optimal directed differentiation, cell phenotype or drug efficacy. We believe these cellular microenvironment characterization and modification techniques can expand upon existing 3D cell culture platforms and allow for new insights into 3D cellular behavior."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/121301"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Hyeon Ryoo"],"dc:subject":["Microgels","3d Cell Culture","Granular Hydrogels","High Throughput Screening","Sensor","Il-6","Ecm"],"dc:title":["Microenvironmental modification and analysis of 3D cell culture using microgels"],"dc:type":["text"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:57Z"}