{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/121278"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/121278","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Aberrant regenerating islet-derived protein 3 (REG3) expression is an early indicator of colonic dysplasia in heterogeneous nuclear ribonucleoprotein (HNRNP I) knockout mice","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01","abstract_has_math":false,"creators":["Liang, Siyuan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Nutritional Sciences","degree_department":null,"school":null,"contributors":["Pan, Yuan-Xiang","Chen, Hong","Swanson, Kelly S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-08","date_published":"2023-08","updated_at":"2026-07-22T22:24:57Z","subjects":["Mice","Regenerating Islet-derived (reg) Gene","Intestinal Epithelial Cell-specific Hnrnp I Knockout","Colonic Dysplasia"],"languages":["en","eng"],"rights":["Copyright 2023 Siyuan Liang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/121278","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pan, Yuan-Xiang","Chen, Hong","Swanson, Kelly S."]},{"key":"dc:creator","label":"Author","values":["Liang, Siyuan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-08","2023-07-19"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Mice","Regenerating Islet-derived (reg) Gene","Intestinal Epithelial Cell-specific Hnrnp I Knockout","Colonic Dysplasia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Siyuan Liang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/121278"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01","The student, Siyuan Liang, accepted the attached license on 2023-07-17 at 15:22.","The student, Siyuan Liang, submitted this Thesis for approval on 2023-07-17 at 15:27.","This Thesis was approved for publication on 2023-07-19 at 16:49.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19714 on 2023-12-04 at 17:35:59","Regenerating islet-derived protein 3 (REG3) acts as multifunctional secretory molecules with pro-proliferative, anti-inflammatory, antimicrobial, and probably cancer-promoting effects. Here, knockout mice with an intestinal epithelial cell (IEC)-specific Hnrnp I gene (KO) ablation were examined, which exhibited an increased risk of developing spontaneous colitis. In this study, we demonstrated that Hnrnp I knockout in mouse intestinal epithelial cells resulted in hyperactive NF-κB signaling, which directly or indirectly downregulated the expression of Reg3b and Reg3g mRNA. We also observed that Hnrnp I knockout in mouse intestinal epithelial cells increased the expression of inflammatory and immune-associated chemokines, including Cxcl9, Cxcl10, Ccl6, and Ccl9. Among them, Cxcl9, Cxcl10, and Ccl9 had similar distribution patterns to Reg3b and Reg3g. Additionally, colonic dysplasia, as a common feature, has been identified in KO mice. We found significant upregulation of REG3B and REG3G protein expression in colonic dysplasia regions in KO mice compared to adjacent normal tissue, indicating that REG3 may serve as a response indicator of colonic dysplasia and an early warning of possible cancer development. The co-localization of GATA3-positive immunofluorescent signals with high levels of REG3 protein expression and colonic dysplasia, along with the identification of GATA3 binding sites in the putative promoter region of the mouse Reg3b gene DNA sequence, collectively indicate the potential regulatory and synergistic role of GATA3 in the development of colonic dysplasia involving REG3. Overall, these findings provide compelling evidence that REG3 has significant potential as an indication for colonic dysplasia."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Aberrant regenerating islet-derived protein 3 (REG3) expression is an early indicator of colonic dysplasia in heterogeneous nuclear ribonucleoprotein (HNRNP I) knockout mice"]}]}],"canonical_facts":{"dc:contributor":["Pan, Yuan-Xiang","Chen, Hong","Swanson, Kelly S."],"dc:creator":["Liang, Siyuan"],"dc:date":["2023-08","2023-07-19"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01","The student, Siyuan Liang, accepted the attached license on 2023-07-17 at 15:22.","The student, Siyuan Liang, submitted this Thesis for approval on 2023-07-17 at 15:27.","This Thesis was approved for publication on 2023-07-19 at 16:49.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19714 on 2023-12-04 at 17:35:59","Regenerating islet-derived protein 3 (REG3) acts as multifunctional secretory molecules with pro-proliferative, anti-inflammatory, antimicrobial, and probably cancer-promoting effects. Here, knockout mice with an intestinal epithelial cell (IEC)-specific Hnrnp I gene (KO) ablation were examined, which exhibited an increased risk of developing spontaneous colitis. In this study, we demonstrated that Hnrnp I knockout in mouse intestinal epithelial cells resulted in hyperactive NF-κB signaling, which directly or indirectly downregulated the expression of Reg3b and Reg3g mRNA. We also observed that Hnrnp I knockout in mouse intestinal epithelial cells increased the expression of inflammatory and immune-associated chemokines, including Cxcl9, Cxcl10, Ccl6, and Ccl9. Among them, Cxcl9, Cxcl10, and Ccl9 had similar distribution patterns to Reg3b and Reg3g. Additionally, colonic dysplasia, as a common feature, has been identified in KO mice. We found significant upregulation of REG3B and REG3G protein expression in colonic dysplasia regions in KO mice compared to adjacent normal tissue, indicating that REG3 may serve as a response indicator of colonic dysplasia and an early warning of possible cancer development. The co-localization of GATA3-positive immunofluorescent signals with high levels of REG3 protein expression and colonic dysplasia, along with the identification of GATA3 binding sites in the putative promoter region of the mouse Reg3b gene DNA sequence, collectively indicate the potential regulatory and synergistic role of GATA3 in the development of colonic dysplasia involving REG3. Overall, these findings provide compelling evidence that REG3 has significant potential as an indication for colonic dysplasia."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/121278"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Siyuan Liang"],"dc:subject":["Mice","Regenerating Islet-derived (reg) Gene","Intestinal Epithelial Cell-specific Hnrnp I Knockout","Colonic Dysplasia"],"dc:title":["Aberrant regenerating islet-derived protein 3 (REG3) expression is an early indicator of colonic dysplasia in heterogeneous nuclear ribonucleoprotein (HNRNP I) knockout mice"],"dc:type":["text"],"thesis:degree_discipline":["Nutritional Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:57Z"}