{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/120588"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/120588","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of the partitioning protein ParB in Caulobacter crescentus","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","abstract_has_math":false,"creators":["Joo, Daniel Lee"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Mera, Paola E"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-05","date_published":"2023-05","updated_at":"2026-07-22T22:24:57Z","subjects":["Chromosome Segregation","Parb","Caulobacter","Cell Cycle"],"languages":["en","eng"],"rights":["Copyright 2023 Daniel Joo"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/120588","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Mera, Paola E"]},{"key":"dc:creator","label":"Author","values":["Joo, Daniel Lee"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-05","2023-05-04"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chromosome Segregation","Parb","Caulobacter","Cell Cycle"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Daniel Joo"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/120588"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","The student, Daniel Joo, accepted the attached license on 2023-05-03 at 10:34.","The student, Daniel Joo, submitted this Thesis for approval on 2023-05-03 at 10:52.","This Thesis was approved for publication on 2023-05-04 at 16:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19313 on 2023-09-01 at 17:22:21","Maintaining chromosome integrity during the cell cycle is essential for bacterial survival. The ParABS partitioning system is involved in coordinating chromosome segregation during bacterial cell cycle. ParB plays an important role in coordinating many cell cycle steps such as chromosome segregation, polar anchoring, loading of the structural maintenance complex protein, and localization of the Z-ring in the bacterial system Caulobacter crescentus (referred here as Caulobacter). However, our understanding of the various potential functions of ParB’s N-terminal domain remains limited. My data indicate that the Caulobacter utilizes the first start codon between the two possible start codons to express the native form of ParB. This discovery is crucial in the field since the two start codons have been used in the literature indiscriminately. I have shown that cells expressing from each start codon display different phenotypes. I have also shown that the expression of the N-terminus truncated parB is lethal even when there is minimal accumulation of this variant protein in the cell. I isolated a suppressor mutant that rescued the lethal effect from the expression of N-terminus truncated parB. Further characterization of this suppressor is needed to determine the mechanism involved in the lethality from expression of N-terminus truncated parB. Further understanding of this key cell cycle regulator and its various functions will advance our knowledge of bacterial cell cycle regulation. This information has the potential to contribute insights to our ability of controlling bacterial growth by manipulating its cell cycle."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Characterization of the partitioning protein ParB in Caulobacter crescentus"]}]}],"canonical_facts":{"dc:contributor":["Mera, Paola E"],"dc:creator":["Joo, Daniel Lee"],"dc:date":["2023-05","2023-05-04"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","The student, Daniel Joo, accepted the attached license on 2023-05-03 at 10:34.","The student, Daniel Joo, submitted this Thesis for approval on 2023-05-03 at 10:52.","This Thesis was approved for publication on 2023-05-04 at 16:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19313 on 2023-09-01 at 17:22:21","Maintaining chromosome integrity during the cell cycle is essential for bacterial survival. The ParABS partitioning system is involved in coordinating chromosome segregation during bacterial cell cycle. ParB plays an important role in coordinating many cell cycle steps such as chromosome segregation, polar anchoring, loading of the structural maintenance complex protein, and localization of the Z-ring in the bacterial system Caulobacter crescentus (referred here as Caulobacter). However, our understanding of the various potential functions of ParB’s N-terminal domain remains limited. My data indicate that the Caulobacter utilizes the first start codon between the two possible start codons to express the native form of ParB. This discovery is crucial in the field since the two start codons have been used in the literature indiscriminately. I have shown that cells expressing from each start codon display different phenotypes. I have also shown that the expression of the N-terminus truncated parB is lethal even when there is minimal accumulation of this variant protein in the cell. I isolated a suppressor mutant that rescued the lethal effect from the expression of N-terminus truncated parB. Further characterization of this suppressor is needed to determine the mechanism involved in the lethality from expression of N-terminus truncated parB. Further understanding of this key cell cycle regulator and its various functions will advance our knowledge of bacterial cell cycle regulation. This information has the potential to contribute insights to our ability of controlling bacterial growth by manipulating its cell cycle."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/120588"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Daniel Joo"],"dc:subject":["Chromosome Segregation","Parb","Caulobacter","Cell Cycle"],"dc:title":["Characterization of the partitioning protein ParB in Caulobacter crescentus"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:57Z"}