{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/120568"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/120568","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The effect of antibiotic resistance on bacterial cell maintenance In gram-negative bacteria","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","abstract_has_math":false,"creators":["Erlandson, Amanda Lee"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Mera, Paola E","Blanke, Steven","Vanderpool, Carin","Slauch, James"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-05","date_published":"2023-05","updated_at":"2026-07-22T22:24:57Z","subjects":["Antibiotic Resistance","Cellular Maintenance","Cell Shape"],"languages":["en","eng"],"rights":["Copyright 2023, Amanda Erlandson"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/120568","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Mera, Paola E","Blanke, Steven","Vanderpool, Carin","Slauch, James"]},{"key":"dc:creator","label":"Author","values":["Erlandson, Amanda Lee"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-05","2023-04-28"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Antibiotic Resistance","Cellular Maintenance","Cell Shape"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023, Amanda Erlandson"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/120568"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","The student, Amanda Erlandson, accepted the attached license on 2023-04-27 at 16:13.","The student, Amanda Erlandson, submitted this Dissertation for approval on 2023-04-27 at 16:36.","This Dissertation was approved for publication on 2023-04-28 at 09:11.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19219 on 2023-09-01 at 17:22:01","Antibiotic resistance has become a global concern, yet many of the mechanisms involved in antibiotic resistance remain poorly understood. Methods of antibiotic resistance are as diverse as antibiotics themselves, ranging from pump-driven mechanisms to eliminate a drug to host repair strategies to mitigate drug-induced damage. The research included in this thesis investigates the characteristics of two forms of antibiotic resistance found in bacteria: response to DNA-intercalators and efflux pumps. We demonstrate in vivo and in vitro that Ecm16 can render resistance against the DNA-intercalator echinomycin. Ecm16 belongs to a sub-class of UvrA-like proteins that are still poorly characterized. This work shows that, like UvrA, Ecm16 functionality requires two ATP-binding sites. Our collaborators have solved its crystal structure allowing a more in-depth assessment of its variation from other UvrA proteins. The second project focuses on physiological changes associated with the antibiotic efflux pump AcrAB-TolC. Although multi-drug efflux has been fairly well characterized, its consequences on cell fitness and development are still largely a mystery. My preliminary data revealed morphological changes in acrAB-tolC overexpression mutants in the presence of lignin-derived vanillate. Although recent research has hinted towards a connection between overexpression of AcrAB-TolC with changes in cell shape, this connection is still undefined. Collectively, the work presented in this thesis provide new insights to the various strategies that bacteria use to become antibiotic resistant."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The effect of antibiotic resistance on bacterial cell maintenance In gram-negative bacteria"]}]}],"canonical_facts":{"dc:contributor":["Mera, Paola E","Blanke, Steven","Vanderpool, Carin","Slauch, James"],"dc:creator":["Erlandson, Amanda Lee"],"dc:date":["2023-05","2023-04-28"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","The student, Amanda Erlandson, accepted the attached license on 2023-04-27 at 16:13.","The student, Amanda Erlandson, submitted this Dissertation for approval on 2023-04-27 at 16:36.","This Dissertation was approved for publication on 2023-04-28 at 09:11.","DSpace SAF Submission Ingestion Package generated from Vireo submission #19219 on 2023-09-01 at 17:22:01","Antibiotic resistance has become a global concern, yet many of the mechanisms involved in antibiotic resistance remain poorly understood. Methods of antibiotic resistance are as diverse as antibiotics themselves, ranging from pump-driven mechanisms to eliminate a drug to host repair strategies to mitigate drug-induced damage. The research included in this thesis investigates the characteristics of two forms of antibiotic resistance found in bacteria: response to DNA-intercalators and efflux pumps. We demonstrate in vivo and in vitro that Ecm16 can render resistance against the DNA-intercalator echinomycin. Ecm16 belongs to a sub-class of UvrA-like proteins that are still poorly characterized. This work shows that, like UvrA, Ecm16 functionality requires two ATP-binding sites. Our collaborators have solved its crystal structure allowing a more in-depth assessment of its variation from other UvrA proteins. The second project focuses on physiological changes associated with the antibiotic efflux pump AcrAB-TolC. Although multi-drug efflux has been fairly well characterized, its consequences on cell fitness and development are still largely a mystery. My preliminary data revealed morphological changes in acrAB-tolC overexpression mutants in the presence of lignin-derived vanillate. Although recent research has hinted towards a connection between overexpression of AcrAB-TolC with changes in cell shape, this connection is still undefined. Collectively, the work presented in this thesis provide new insights to the various strategies that bacteria use to become antibiotic resistant."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/120568"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023, Amanda Erlandson"],"dc:subject":["Antibiotic Resistance","Cellular Maintenance","Cell Shape"],"dc:title":["The effect of antibiotic resistance on bacterial cell maintenance In gram-negative bacteria"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:57Z"}