{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/120515"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/120515","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Genomic regulation of social behavior in eusocial bees","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","abstract_has_math":false,"creators":["Stuart, Sarai Hope"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Ecol, Evol, Conservation Biol","degree_department":null,"school":null,"contributors":["Robinson, Gene E","Bell, Alison M","Stubbs, Lisa J","Sinhha, Saurabh"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-05","date_published":"2023-05","updated_at":"2026-07-22T22:24:57Z","subjects":["Division Of Labor","Social Bees","Functional Genomics","Microrna","Apis Mellifera","Stable Lipid Loss","Tetragonisca Angustula","Gene Regulation","Alarm Pheromone","Comparative Transcriptomics"],"languages":["en","eng"],"rights":["Copyright 2023 Sarai Stuart"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/120515","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Robinson, Gene E","Bell, Alison M","Stubbs, Lisa J","Sinhha, Saurabh"]},{"key":"dc:creator","label":"Author","values":["Stuart, Sarai Hope"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2023-05","2023-04-16"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Ecol, Evol, Conservation Biol"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Division Of Labor","Social Bees","Functional Genomics","Microrna","Apis Mellifera","Stable Lipid Loss","Tetragonisca Angustula","Gene Regulation","Alarm Pheromone","Comparative Transcriptomics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2023 Sarai Stuart"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/120515"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","The student, Sarai Stuart, accepted the attached license on 2023-04-16 at 11:37.","The student, Sarai Stuart, submitted this Dissertation for approval on 2023-04-16 at 12:00.","This Dissertation was approved for publication on 2023-04-16 at 14:14.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18998 on 2023-09-01 at 17:20:29","The discovery of regulatory molecules that alter gene expression without changes to DNA sequence fundamentally altered how we think about the regulation of organismal processes. Work in this dissertation leverages naturally occurring behavioral plasticity in two species of highly social bees -- Apis mellifera (Chapters 2-5) and Tetragonisca angustula (Chapter 5) -- to investigate gene expression and gene regulatory mechanisms underlying division of labor among adult worker bees. Chapter 1 provides an overview of experimental approaches and work contained in this dissertation. Chapter 2 uses a combination of bioinformatic and transcriptomic screens to identify candidate microRNAs (miRNAs) involved in A. mellifera abdominal lipid loss, a key physiological phenotype linked to division of labor. This chapter additionally describes the development of antagomirs for in vivo decrease in the expression of two miRNAs and quantifies abdominal lipid level. In Chapter 3 antagomir treatment is used in combination with juvenile hormone and queen mandibular pheromone treatments to probe whether these factors interact with miRNA ame-miR-305-5p to influence lipid mass. Chapter 4 examines the effects of ame-miR-305-5p knockdown with antagomir on total transcriptomic profiles in the abdominal fat body and brain. Finally, Chapter 5 uses comparative transcriptomic analyses of A. mellifera and T. angustula to explore the question of whether mechanisms of colony defense behavior in these two distantly related species of bees relies upon common molecular mechanisms. Together the studies contained within this dissertation contribute to our understanding of the regulation of the division of labor and the evolution of complex behavioral traits."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Genomic regulation of social behavior in eusocial bees"]}]}],"canonical_facts":{"dc:contributor":["Robinson, Gene E","Bell, Alison M","Stubbs, Lisa J","Sinhha, Saurabh"],"dc:creator":["Stuart, Sarai Hope"],"dc:date":["2023-05","2023-04-16"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01","The student, Sarai Stuart, accepted the attached license on 2023-04-16 at 11:37.","The student, Sarai Stuart, submitted this Dissertation for approval on 2023-04-16 at 12:00.","This Dissertation was approved for publication on 2023-04-16 at 14:14.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18998 on 2023-09-01 at 17:20:29","The discovery of regulatory molecules that alter gene expression without changes to DNA sequence fundamentally altered how we think about the regulation of organismal processes. Work in this dissertation leverages naturally occurring behavioral plasticity in two species of highly social bees -- Apis mellifera (Chapters 2-5) and Tetragonisca angustula (Chapter 5) -- to investigate gene expression and gene regulatory mechanisms underlying division of labor among adult worker bees. Chapter 1 provides an overview of experimental approaches and work contained in this dissertation. Chapter 2 uses a combination of bioinformatic and transcriptomic screens to identify candidate microRNAs (miRNAs) involved in A. mellifera abdominal lipid loss, a key physiological phenotype linked to division of labor. This chapter additionally describes the development of antagomirs for in vivo decrease in the expression of two miRNAs and quantifies abdominal lipid level. In Chapter 3 antagomir treatment is used in combination with juvenile hormone and queen mandibular pheromone treatments to probe whether these factors interact with miRNA ame-miR-305-5p to influence lipid mass. Chapter 4 examines the effects of ame-miR-305-5p knockdown with antagomir on total transcriptomic profiles in the abdominal fat body and brain. Finally, Chapter 5 uses comparative transcriptomic analyses of A. mellifera and T. angustula to explore the question of whether mechanisms of colony defense behavior in these two distantly related species of bees relies upon common molecular mechanisms. Together the studies contained within this dissertation contribute to our understanding of the regulation of the division of labor and the evolution of complex behavioral traits."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/120515"],"dc:language":["en","eng"],"dc:rights":["Copyright 2023 Sarai Stuart"],"dc:subject":["Division Of Labor","Social Bees","Functional Genomics","Microrna","Apis Mellifera","Stable Lipid Loss","Tetragonisca Angustula","Gene Regulation","Alarm Pheromone","Comparative Transcriptomics"],"dc:title":["Genomic regulation of social behavior in eusocial bees"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Ecol, Evol, Conservation Biol"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:57Z"}