{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/117595"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/117595","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Modification of substrate specificity of thioesterase, reductase, desaturase by MALDI-tof-MS analysis of microbial colonies","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01","abstract_has_math":false,"creators":["Choe, Kisurb"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Sweedler, Jonathan","Nair, Satish","Procko , Erik","Zhao, Huimin"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-12","date_published":"2022-12","updated_at":"2026-07-22T22:24:56Z","subjects":["Enzymes","Mass Spectrometry","Maldi-ms"],"languages":["en","eng"],"rights":["Copyright 2022 Kisurb Choe"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/117595","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sweedler, Jonathan","Nair, Satish","Procko , Erik","Zhao, Huimin"]},{"key":"dc:creator","label":"Author","values":["Choe, Kisurb"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-12","2022-12-02"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Enzymes","Mass Spectrometry","Maldi-ms"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Kisurb Choe"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/117595"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01","The student, Kisurb Choe, accepted the attached license on 2022-12-01 at 23:45.","The student, Kisurb Choe, submitted this Dissertation for approval on 2022-12-01 at 23:59.","This Dissertation was approved for publication on 2022-12-02 at 16:17.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18732 on 2023-04-12 at 11:36:48","Chemical heterogeneity in biological systems reveal important clues to the inner workings of the fundamental processes such as lipid metabolism and physiology. High throughput screening of microbes and mutant libraries resolve such complexities enabling multiple biochemical studies such as directed evolution, functional genomics, microbial identification, and genome mining. A promising screening approach is measurement of biochemical products directly from microbial colonies via optically guided matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Measuring the compounds from microbial colonies bypasses liquid culture while minimizing the automation processes, and the wide chemical coverage and the high sensitivity of MALDI-MS can enable diverse biochemical screening projects such as modifying enzymes and functional genomics survey of gene activation/inhibition libraries. The thesis first presents the informatics and the experimental methods that were created to develop the MALDI-MS based colony screen. The macroMS software was developed to perform informatics tasks such as image analysis finding samples, MALDI-MS automation targeting the samples, and data analysis / visualization to find an analyte of interest. Then an experimental method for colony sample preparation, constructing sprayer for applying MALDI matrix, and running MALDI-MS is described. Based on the colony screening method, random libraries of an acyl-ACP thioesterase, an acyl-ACP desaturase, an acyl-CoA reductase were scanned, revealing novel variants with increased specificity for specific products."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Modification of substrate specificity of thioesterase, reductase, desaturase by MALDI-tof-MS analysis of microbial colonies"]}]}],"canonical_facts":{"dc:contributor":["Sweedler, Jonathan","Nair, Satish","Procko , Erik","Zhao, Huimin"],"dc:creator":["Choe, Kisurb"],"dc:date":["2022-12","2022-12-02"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01","The student, Kisurb Choe, accepted the attached license on 2022-12-01 at 23:45.","The student, Kisurb Choe, submitted this Dissertation for approval on 2022-12-01 at 23:59.","This Dissertation was approved for publication on 2022-12-02 at 16:17.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18732 on 2023-04-12 at 11:36:48","Chemical heterogeneity in biological systems reveal important clues to the inner workings of the fundamental processes such as lipid metabolism and physiology. High throughput screening of microbes and mutant libraries resolve such complexities enabling multiple biochemical studies such as directed evolution, functional genomics, microbial identification, and genome mining. A promising screening approach is measurement of biochemical products directly from microbial colonies via optically guided matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Measuring the compounds from microbial colonies bypasses liquid culture while minimizing the automation processes, and the wide chemical coverage and the high sensitivity of MALDI-MS can enable diverse biochemical screening projects such as modifying enzymes and functional genomics survey of gene activation/inhibition libraries. The thesis first presents the informatics and the experimental methods that were created to develop the MALDI-MS based colony screen. The macroMS software was developed to perform informatics tasks such as image analysis finding samples, MALDI-MS automation targeting the samples, and data analysis / visualization to find an analyte of interest. Then an experimental method for colony sample preparation, constructing sprayer for applying MALDI matrix, and running MALDI-MS is described. Based on the colony screening method, random libraries of an acyl-ACP thioesterase, an acyl-ACP desaturase, an acyl-CoA reductase were scanned, revealing novel variants with increased specificity for specific products."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/117595"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Kisurb Choe"],"dc:subject":["Enzymes","Mass Spectrometry","Maldi-ms"],"dc:title":["Modification of substrate specificity of thioesterase, reductase, desaturase by MALDI-tof-MS analysis of microbial colonies"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:56Z"}