{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/117588"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/117588","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Comparative mechanistic study of Coxsackievirus B5 and Human Adenovirus Serotype 2 inactivation by chlorine","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01","abstract_has_math":false,"creators":["Cong, Wen"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Environ Engr in Civil Engr","degree_department":null,"school":null,"contributors":["Mariñas, Benito Jose","Liu, Wen-Tso","Wei, Na","Lu, Yi"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-12","date_published":"2022-12","updated_at":"2026-07-22T22:24:56Z","subjects":["Enterovirus","Coxsackievirus","Adenovirus","Chlorine","Free Chlorine","Combined Chlorine","Chloramine","Disinfection","Water","Kinetics","Replication Cycle","Replication Cycle Analysis","Gene Damage","Genome Damage","Naked Gene","Naked Genome","Attachment","Gene Replication"],"languages":["en","eng"],"rights":["Copyright 2022 Wen Cong"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/117588","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Mariñas, Benito Jose","Liu, Wen-Tso","Wei, Na","Lu, Yi"]},{"key":"dc:creator","label":"Author","values":["Cong, Wen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-12","2022-12-01"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environ Engr in Civil Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Enterovirus","Coxsackievirus","Adenovirus","Chlorine","Free Chlorine","Combined Chlorine","Chloramine","Disinfection","Water","Kinetics","Replication Cycle","Replication Cycle Analysis","Gene Damage","Genome Damage","Naked Gene","Naked Genome","Attachment","Gene Replication"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Wen Cong"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/117588"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01","The student, Wen Cong, accepted the attached license on 2022-12-01 at 09:17.","The student, Wen Cong, submitted this Dissertation for approval on 2022-12-01 at 09:26.","This Dissertation was approved for publication on 2022-12-01 at 17:18.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18711 on 2023-04-12 at 11:36:22","Effective control of waterborne pathogens is a primary goal toward providing safe drinking water. Viral pathogens are of particular concern because of challenges associated with detecting and monitoring infectious virions. US Environmental Protection Agency (USEPA) requires 4-log removal of enteric viruses by a combination of filtration and disinfection. Chlorine, including free chlorine and chloramine (mainly monochloramine), are two widely used disinfectants. Coxsackievirus B5 (CVB5), a single-stranded RNA virus, is one of the most resistant viruses to free chlorine treatment. Human Adenovirus Serotype 2 (HAdV-2), a double-stranded DNA virus, is the most resistant virus to monochloramine treatment, while being sensitive to free chlorine. On the basis of previous studies, this dissertation presents a comparative study of CVB5 and HAdV-2 inactivation by free chlorine or monochloramine, on the kinetics and molecular mechanistic levels. Inactivation kinetics of CVB5 inactivation by monochloramine (the main species of chloramines) at various pH and temperature conditions were examined. Mathematical models were built based on the CVB5 free chlorine and monochloramine inactivation data to predict the CVB5 inactivation rate constants and the kinetics in pH and temperature ranges relevant to water treatment by free chlorine or monochloramine. The genome integrity damage of CVB5 by free chlorine or monochloramine with exposure relevant to water treatment or several times higher was examined by quantitative PCR with approximately 100 or 1000-base amplicons. The effect of free chlorine or monochloramine treatment on several CVB5 replication cycle events were investigated. Additionally, plaque assays with serial transfer were performed for HAdV-2 to determine its ratio of Infectious Virion-to-PFU, which is helpful for the estimation of infectious HAdV-2 concentrations with conventional plaque assay. Overall, this dissertation provides a comprehensive picture of CVB5 and HAdV-2 inactivation by free chlorine and monochloramine in the aspect of kinetics, genome damage and replication cycle events, with a focus on CVB5 studies. Future legislation of water disinfection and development of new technologies such as infectious virus sensor could benefit from these research outcomes."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Comparative mechanistic study of Coxsackievirus B5 and Human Adenovirus Serotype 2 inactivation by chlorine"]}]}],"canonical_facts":{"dc:contributor":["Mariñas, Benito Jose","Liu, Wen-Tso","Wei, Na","Lu, Yi"],"dc:creator":["Cong, Wen"],"dc:date":["2022-12","2022-12-01"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01","The student, Wen Cong, accepted the attached license on 2022-12-01 at 09:17.","The student, Wen Cong, submitted this Dissertation for approval on 2022-12-01 at 09:26.","This Dissertation was approved for publication on 2022-12-01 at 17:18.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18711 on 2023-04-12 at 11:36:22","Effective control of waterborne pathogens is a primary goal toward providing safe drinking water. Viral pathogens are of particular concern because of challenges associated with detecting and monitoring infectious virions. US Environmental Protection Agency (USEPA) requires 4-log removal of enteric viruses by a combination of filtration and disinfection. Chlorine, including free chlorine and chloramine (mainly monochloramine), are two widely used disinfectants. Coxsackievirus B5 (CVB5), a single-stranded RNA virus, is one of the most resistant viruses to free chlorine treatment. Human Adenovirus Serotype 2 (HAdV-2), a double-stranded DNA virus, is the most resistant virus to monochloramine treatment, while being sensitive to free chlorine. On the basis of previous studies, this dissertation presents a comparative study of CVB5 and HAdV-2 inactivation by free chlorine or monochloramine, on the kinetics and molecular mechanistic levels. Inactivation kinetics of CVB5 inactivation by monochloramine (the main species of chloramines) at various pH and temperature conditions were examined. Mathematical models were built based on the CVB5 free chlorine and monochloramine inactivation data to predict the CVB5 inactivation rate constants and the kinetics in pH and temperature ranges relevant to water treatment by free chlorine or monochloramine. The genome integrity damage of CVB5 by free chlorine or monochloramine with exposure relevant to water treatment or several times higher was examined by quantitative PCR with approximately 100 or 1000-base amplicons. The effect of free chlorine or monochloramine treatment on several CVB5 replication cycle events were investigated. Additionally, plaque assays with serial transfer were performed for HAdV-2 to determine its ratio of Infectious Virion-to-PFU, which is helpful for the estimation of infectious HAdV-2 concentrations with conventional plaque assay. Overall, this dissertation provides a comprehensive picture of CVB5 and HAdV-2 inactivation by free chlorine and monochloramine in the aspect of kinetics, genome damage and replication cycle events, with a focus on CVB5 studies. Future legislation of water disinfection and development of new technologies such as infectious virus sensor could benefit from these research outcomes."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/117588"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Wen Cong"],"dc:subject":["Enterovirus","Coxsackievirus","Adenovirus","Chlorine","Free Chlorine","Combined Chlorine","Chloramine","Disinfection","Water","Kinetics","Replication Cycle","Replication Cycle Analysis","Gene Damage","Genome Damage","Naked Gene","Naked Genome","Attachment","Gene Replication"],"dc:title":["Comparative mechanistic study of Coxsackievirus B5 and Human Adenovirus Serotype 2 inactivation by chlorine"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Environ Engr in Civil Engr"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:56Z"}