{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/116156"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/116156","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Mineralized collagen biomaterials for studying bone biology in vitro","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo terms","abstract_has_math":false,"creators":["Tiffany, Aleczandria Skye"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemical Engineering","degree_department":null,"school":null,"contributors":["Harley, Brendan","Kraft, Mary","Kong, Hyunjoon","Wagoner-Johnson, Amy"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-08","date_published":"2022-08","updated_at":"2026-07-22T22:24:55Z","subjects":["bone","tissue engineering","osteogenesis","growth plate"],"languages":["en","eng"],"rights":["Copyright 2022 Aleczandria Tiffany"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/116156","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Harley, Brendan","Kraft, Mary","Kong, Hyunjoon","Wagoner-Johnson, Amy"]},{"key":"dc:creator","label":"Author","values":["Tiffany, Aleczandria Skye"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-08","2022-06-22"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["bone","tissue engineering","osteogenesis","growth plate"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Aleczandria Tiffany"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/116156"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo terms","The student, Aleczandria Tiffany, accepted the attached license on 2022-06-09 at 20:03.","The student, Aleczandria Tiffany, submitted this Dissertation for approval on 2022-06-09 at 20:05.","This Dissertation was approved for publication on 2022-06-22 at 15:58.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18066 on 2022-11-15 at 17:37:32","Craniomaxillofacial injuries are injuries to the neck, face, and jaw. Trauma-induced craniomaxillofacial injuries present unique clinical challenges because they are oftentimes large and complex in size and shape. Clinical solutions, the autograft and allograft, are inadequate due to tissue availability (autograft) and variability in purification methods (allograft). Tissue engineering strategies are needed to develop implants that can regenerate these large-scale craniomaxillofacial injures. Our lab has developed a class of mineralized collagen scaffolds that contain collagen, glycosaminoglycans, and a calcium phosphate mineral phase. These materials promote osteogenesis in vitro and in vivo, but we have recently observed poor healing in a critical size in vivo mandible injury due to poor cell infiltration, osteogenesis, and vascular ingrowth. Thus, this dissertation focuses on the enhancement of our mineralized collagen platform to improve and evaluate cell metabolic health and proliferation, osteogenesis, and angiogenesis. We first describe material changes regarding mineral content and growth factor supplementation with the goal to improve mesenchymal stem cell proliferation and osteogenesis in vitro and in vivo. Further, we describe a comprehensive study of donor variability within our materials. This work was motivated by the need to have consistent healing between patients, and we demonstrate the ability to evaluate osteogenic potential of mesenchymal stem cells from eight donors. We then transition into the culture of other relevant cell types in the bone microenvironment: endothelial cells and osteoclasts. Here, we illustrate the potential of our mineralized collagen scaffolds as complex cell culture systems and discuss the future of our materials to study cell-cell interactions and disease progression in vitro. Finally, we motivate a paradigm shift in how we design materials for bone repair by discussing a developmental tissue that is critical for skeletal growth: the growth plates. Overall, this dissertation describes material changes to influence cell activity and proliferation and the potential of our materials to model bone repair processes in vitro."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Mineralized collagen biomaterials for studying bone biology in vitro"]}]}],"canonical_facts":{"dc:contributor":["Harley, Brendan","Kraft, Mary","Kong, Hyunjoon","Wagoner-Johnson, Amy"],"dc:creator":["Tiffany, Aleczandria Skye"],"dc:date":["2022-08","2022-06-22"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo terms","The student, Aleczandria Tiffany, accepted the attached license on 2022-06-09 at 20:03.","The student, Aleczandria Tiffany, submitted this Dissertation for approval on 2022-06-09 at 20:05.","This Dissertation was approved for publication on 2022-06-22 at 15:58.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18066 on 2022-11-15 at 17:37:32","Craniomaxillofacial injuries are injuries to the neck, face, and jaw. Trauma-induced craniomaxillofacial injuries present unique clinical challenges because they are oftentimes large and complex in size and shape. Clinical solutions, the autograft and allograft, are inadequate due to tissue availability (autograft) and variability in purification methods (allograft). Tissue engineering strategies are needed to develop implants that can regenerate these large-scale craniomaxillofacial injures. Our lab has developed a class of mineralized collagen scaffolds that contain collagen, glycosaminoglycans, and a calcium phosphate mineral phase. These materials promote osteogenesis in vitro and in vivo, but we have recently observed poor healing in a critical size in vivo mandible injury due to poor cell infiltration, osteogenesis, and vascular ingrowth. Thus, this dissertation focuses on the enhancement of our mineralized collagen platform to improve and evaluate cell metabolic health and proliferation, osteogenesis, and angiogenesis. We first describe material changes regarding mineral content and growth factor supplementation with the goal to improve mesenchymal stem cell proliferation and osteogenesis in vitro and in vivo. Further, we describe a comprehensive study of donor variability within our materials. This work was motivated by the need to have consistent healing between patients, and we demonstrate the ability to evaluate osteogenic potential of mesenchymal stem cells from eight donors. We then transition into the culture of other relevant cell types in the bone microenvironment: endothelial cells and osteoclasts. Here, we illustrate the potential of our mineralized collagen scaffolds as complex cell culture systems and discuss the future of our materials to study cell-cell interactions and disease progression in vitro. Finally, we motivate a paradigm shift in how we design materials for bone repair by discussing a developmental tissue that is critical for skeletal growth: the growth plates. Overall, this dissertation describes material changes to influence cell activity and proliferation and the potential of our materials to model bone repair processes in vitro."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/116156"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Aleczandria Tiffany"],"dc:subject":["bone","tissue engineering","osteogenesis","growth plate"],"dc:title":["Mineralized collagen biomaterials for studying bone biology in vitro"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Chemical Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:55Z"}