{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/116135"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/116135","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Rational design of chemical tools for in vivo applications via photoacoustic imaging","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo terms","abstract_has_math":false,"creators":["Lucero, Melissa"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Chan, Jefferson K","Zimmerman, Steven","Han, Hee-Sun","Chen, Yun-Sheng"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-08","date_published":"2022-08","updated_at":"2026-07-22T22:24:55Z","subjects":["In vivo","photoacoustic","imaging","activity-based","sensing","biomedical"],"languages":["en","eng"],"rights":["Copyright 2022 Melissa Lucero"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/116135","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Chan, Jefferson K","Zimmerman, Steven","Han, Hee-Sun","Chen, Yun-Sheng"]},{"key":"dc:creator","label":"Author","values":["Lucero, Melissa"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-08","2022-04-22"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["In vivo","photoacoustic","imaging","activity-based","sensing","biomedical"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Melissa Lucero"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/116135"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo terms","The student, Melissa Lucero, accepted the attached license on 2022-04-20 at 12:51.","The student, Melissa Lucero, submitted this Dissertation for approval on 2022-04-20 at 13:42.","This Dissertation was approved for publication on 2022-04-22 at 14:59.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17844 on 2022-11-15 at 17:37:22","The combination of activity-based sensing and photoacoustic imaging has allowed for the development of probes that can be used to image enzyme activity (e.g., NTR, FAAH, and MGL), reactive species (e.g., peroxynitrite, nitric oxide, and glutathione) and metal ions (e.g., iron, copper, and calcium). Activity-based sensing leverages the chemical reactivity of biological targets to elicit a readout that can be used to assess the activity of the target. Work in this area has proven to be essential for understanding biological processes and diseases in their native environment. This is made possible by photoacoustic imaging which uses safe, non-ionizing light to generate ultrasound in tissue of live animals. Chapter 1 will introduce how activity-based sensing has been used in the development of various photoacoustic probes. Specifically, this chapter will discuss the design and development of initial work in this area. Chapter 2 will describe work in developing photoacoustic tools for copper sensing in various human disease states. Chapter 3 presents the first application of photoacoustic imaging in the development of companion diagnostics. Importantly, this work introduces a physical organic approach towards probe development. Chapter 4 further establishes the physical organic approach with work that introduces first small-molecule NIR-II photoacoustic probe for in vivo sensing of nitric oxide in murine models of cancer. Lastly, chapter 5 discusses work on understanding the effects of lifestyle habits on inflammation by measuring the activity of fatty acid metabolism using enzyme specific probes. The work herein describes vast applications of photoacoustic imaging and promise in the clinical setting."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Rational design of chemical tools for in vivo applications via photoacoustic imaging"]}]}],"canonical_facts":{"dc:contributor":["Chan, Jefferson K","Zimmerman, Steven","Han, Hee-Sun","Chen, Yun-Sheng"],"dc:creator":["Lucero, Melissa"],"dc:date":["2022-08","2022-04-22"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo terms","The student, Melissa Lucero, accepted the attached license on 2022-04-20 at 12:51.","The student, Melissa Lucero, submitted this Dissertation for approval on 2022-04-20 at 13:42.","This Dissertation was approved for publication on 2022-04-22 at 14:59.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17844 on 2022-11-15 at 17:37:22","The combination of activity-based sensing and photoacoustic imaging has allowed for the development of probes that can be used to image enzyme activity (e.g., NTR, FAAH, and MGL), reactive species (e.g., peroxynitrite, nitric oxide, and glutathione) and metal ions (e.g., iron, copper, and calcium). Activity-based sensing leverages the chemical reactivity of biological targets to elicit a readout that can be used to assess the activity of the target. Work in this area has proven to be essential for understanding biological processes and diseases in their native environment. This is made possible by photoacoustic imaging which uses safe, non-ionizing light to generate ultrasound in tissue of live animals. Chapter 1 will introduce how activity-based sensing has been used in the development of various photoacoustic probes. Specifically, this chapter will discuss the design and development of initial work in this area. Chapter 2 will describe work in developing photoacoustic tools for copper sensing in various human disease states. Chapter 3 presents the first application of photoacoustic imaging in the development of companion diagnostics. Importantly, this work introduces a physical organic approach towards probe development. Chapter 4 further establishes the physical organic approach with work that introduces first small-molecule NIR-II photoacoustic probe for in vivo sensing of nitric oxide in murine models of cancer. Lastly, chapter 5 discusses work on understanding the effects of lifestyle habits on inflammation by measuring the activity of fatty acid metabolism using enzyme specific probes. The work herein describes vast applications of photoacoustic imaging and promise in the clinical setting."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/116135"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Melissa Lucero"],"dc:subject":["In vivo","photoacoustic","imaging","activity-based","sensing","biomedical"],"dc:title":["Rational design of chemical tools for in vivo applications via photoacoustic imaging"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:55Z"}