{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/116033"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/116033","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Dissecting nuclear protein dynamics and structural changes with single-molecule spectroscopy and microscopy","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-08-01","abstract_has_math":false,"creators":["Liu, Wenjie"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Irudayaraj, Joseph","Cunningham, Brian","Underhill, Gregory","Bhargava, Rohit"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-08","date_published":"2022-08","updated_at":"2026-07-22T22:24:55Z","subjects":["epigenetics","single-molecule"],"languages":["en","eng"],"rights":["Copyright 2022 Wenjie Liu"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/116033","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Irudayaraj, Joseph","Cunningham, Brian","Underhill, Gregory","Bhargava, Rohit"]},{"key":"dc:creator","label":"Author","values":["Liu, Wenjie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-08","2022-06-29"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["epigenetics","single-molecule"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Wenjie Liu"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/116033"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-08-01","The student, Wenjie Liu, accepted the attached license on 2022-06-20 at 15:32.","The student, Wenjie Liu, submitted this Dissertation for approval on 2022-06-20 at 15:40.","This Dissertation was approved for publication on 2022-06-29 at 09:17.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18084 on 2022-11-15 at 19:16:40","Cancer is one of the most significant causes of mortality in the United States, with approximately 1 in 4 individuals likely to develop cancer during their lifespan. Unfortunately, despite the enormous effort in cancer diagnosis and therapy, the mortality rate is still high because of multiple challenges imposed by cancer relapse, metastasis, and drug resistance. The origin of such tragedy lies in the heterogeneity of tumors featuring various cellular morphology, genomics, and epigenomics. Tumor heterogeneity is linked with genetic mutations, epigenetic alterations, and external pressure from the tumor microenvironment. Biochemical approaches such as next-generation sequencing can elucidate the genetic origins of heterogeneity. Recent literature has shown that epigenetic modifications play a prominent role in modulating transcription, yet limited knowledge exists in the mechanistic understanding of such changes in the context of living cell. Understanding how epigenetic modifications affect the state of chromatin and discovering phenotypical epigenetic states are critical for advanced cancer detection and treatment. This work evaluated how external cues, such as environmental toxicants, pharmaceutical drugs, and tumor microenvironment, could affect chromatin and its dynamic states with single-molecule fluorescence tools in living cells. To develop a mechanistic basis, single-molecule tools were used to track and identify cells that exhibit phenotypical epigenetic traits, linking specific epigenetic modifications in response to drug treatment and tumor progression."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Dissecting nuclear protein dynamics and structural changes with single-molecule spectroscopy and microscopy"]}]}],"canonical_facts":{"dc:contributor":["Irudayaraj, Joseph","Cunningham, Brian","Underhill, Gregory","Bhargava, Rohit"],"dc:creator":["Liu, Wenjie"],"dc:date":["2022-08","2022-06-29"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-08-01","The student, Wenjie Liu, accepted the attached license on 2022-06-20 at 15:32.","The student, Wenjie Liu, submitted this Dissertation for approval on 2022-06-20 at 15:40.","This Dissertation was approved for publication on 2022-06-29 at 09:17.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18084 on 2022-11-15 at 19:16:40","Cancer is one of the most significant causes of mortality in the United States, with approximately 1 in 4 individuals likely to develop cancer during their lifespan. Unfortunately, despite the enormous effort in cancer diagnosis and therapy, the mortality rate is still high because of multiple challenges imposed by cancer relapse, metastasis, and drug resistance. The origin of such tragedy lies in the heterogeneity of tumors featuring various cellular morphology, genomics, and epigenomics. Tumor heterogeneity is linked with genetic mutations, epigenetic alterations, and external pressure from the tumor microenvironment. Biochemical approaches such as next-generation sequencing can elucidate the genetic origins of heterogeneity. Recent literature has shown that epigenetic modifications play a prominent role in modulating transcription, yet limited knowledge exists in the mechanistic understanding of such changes in the context of living cell. Understanding how epigenetic modifications affect the state of chromatin and discovering phenotypical epigenetic states are critical for advanced cancer detection and treatment. This work evaluated how external cues, such as environmental toxicants, pharmaceutical drugs, and tumor microenvironment, could affect chromatin and its dynamic states with single-molecule fluorescence tools in living cells. To develop a mechanistic basis, single-molecule tools were used to track and identify cells that exhibit phenotypical epigenetic traits, linking specific epigenetic modifications in response to drug treatment and tumor progression."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/116033"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Wenjie Liu"],"dc:subject":["epigenetics","single-molecule"],"dc:title":["Dissecting nuclear protein dynamics and structural changes with single-molecule spectroscopy and microscopy"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:55Z"}