{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/115946"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/115946","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Mechanoprobe fabrication and development for mechanobiology","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01","abstract_has_math":false,"creators":["Mohagheghian, Erfan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Mechanical Engineering","degree_department":null,"school":null,"contributors":["Wang, Ning","Ewoldt, Randy","Johnson, Amy Wagoner","Underhill, Gregory"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-08","date_published":"2022-08","updated_at":"2026-07-22T22:24:55Z","subjects":["Mechanobiology","Cell traction","Cell and Tissue Stiffness"],"languages":["en","eng"],"rights":["Copyright 2022 Erfan Mohagheghian"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/115946","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wang, Ning","Ewoldt, Randy","Johnson, Amy Wagoner","Underhill, Gregory"]},{"key":"dc:creator","label":"Author","values":["Mohagheghian, Erfan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-08","2022-07-15"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Mechanical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Mechanobiology","Cell traction","Cell and Tissue Stiffness"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Erfan Mohagheghian"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/115946"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01","The student, Erfan Mohagheghian, accepted the attached license on 2022-07-15 at 12:33.","The student, Erfan Mohagheghian, submitted this Dissertation for approval on 2022-07-15 at 12:45.","This Dissertation was approved for publication on 2022-07-15 at 15:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18334 on 2022-11-16 at 10:56:28","Mounting evidence supports the notion that mechanical cues are one of the key regulators of cell functions driving many important biological phenomena like development, morphogenesis, differentiation, and disease. As a result, further progress in full understanding of the role of mechanics in biology requires the technologies to quantify tractions and stiffness as two essential parameters in physiology and pathology. Despite the extensive efforts in recent decades, it has been yet extremely challenging to quantify both traction and stiffness in vivo in a living animal. To address this challenge, we first introduce the fluorescent nanoparticle-labeled, mono-disperse biocompatible, elastic round microgel (ERMG) of Arg-Gly-Asp conjugated alginate hydrogels as a passive force sensor to quantify compressive, tensile stresses, and shear stresses in cancer cell layer, and cancer cell colony. The exerted force by surrounding cells deformed the soft ERMG (1.4 kPa Young’s modulus), and the deformation was captured using digital volume correlation comparing the stressed and stress-free microgel. Subsequently, the strain, stress, and applied traction were calculated from the cell-induced deformation of the microgel. We further extend our approach by developing a novel mechanomicrorobot with controllable functionality via magnetic fields to measure cellular tractions and the mechanical properties of the microenvironment in live 3D tissue in the same location in vivo. The mechanomicrorobot is the result of integrating two ideas: elastic round microgel (ERMG) and magnetic twisting cytometry (MTC) by fabrication of biocompatible ferromagnetic micro-structure and its encapsulation in photodegradable elastic round microgel. In this approach, the initially stiff mechanomicrorobot is actuated by an oscillating twisting magnetic field to probe the microenvironment by its rigid rotation. Following the stiffness measurement, the mechanomicrorobot can be dynamically softened by controlling the UV exposure time to tune its modulus and probe a wide range of 3D tractions generated by a biological sample. Using this novel approach, we demonstrate that mechanomicrorobot probe can reveal distinct modulus and tractions in tumor cell colonies. Collectively, these results strongly suggest that ERMG and mechanomicrorobot can be used in a broad range of in vivo studies in mechanobiology and provide future insights into the role of mechanical factors in tumor progression and organogenesis during embryonic development."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Mechanoprobe fabrication and development for mechanobiology"]}]}],"canonical_facts":{"dc:contributor":["Wang, Ning","Ewoldt, Randy","Johnson, Amy Wagoner","Underhill, Gregory"],"dc:creator":["Mohagheghian, Erfan"],"dc:date":["2022-08","2022-07-15"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01","The student, Erfan Mohagheghian, accepted the attached license on 2022-07-15 at 12:33.","The student, Erfan Mohagheghian, submitted this Dissertation for approval on 2022-07-15 at 12:45.","This Dissertation was approved for publication on 2022-07-15 at 15:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18334 on 2022-11-16 at 10:56:28","Mounting evidence supports the notion that mechanical cues are one of the key regulators of cell functions driving many important biological phenomena like development, morphogenesis, differentiation, and disease. As a result, further progress in full understanding of the role of mechanics in biology requires the technologies to quantify tractions and stiffness as two essential parameters in physiology and pathology. Despite the extensive efforts in recent decades, it has been yet extremely challenging to quantify both traction and stiffness in vivo in a living animal. To address this challenge, we first introduce the fluorescent nanoparticle-labeled, mono-disperse biocompatible, elastic round microgel (ERMG) of Arg-Gly-Asp conjugated alginate hydrogels as a passive force sensor to quantify compressive, tensile stresses, and shear stresses in cancer cell layer, and cancer cell colony. The exerted force by surrounding cells deformed the soft ERMG (1.4 kPa Young’s modulus), and the deformation was captured using digital volume correlation comparing the stressed and stress-free microgel. Subsequently, the strain, stress, and applied traction were calculated from the cell-induced deformation of the microgel. We further extend our approach by developing a novel mechanomicrorobot with controllable functionality via magnetic fields to measure cellular tractions and the mechanical properties of the microenvironment in live 3D tissue in the same location in vivo. The mechanomicrorobot is the result of integrating two ideas: elastic round microgel (ERMG) and magnetic twisting cytometry (MTC) by fabrication of biocompatible ferromagnetic micro-structure and its encapsulation in photodegradable elastic round microgel. In this approach, the initially stiff mechanomicrorobot is actuated by an oscillating twisting magnetic field to probe the microenvironment by its rigid rotation. Following the stiffness measurement, the mechanomicrorobot can be dynamically softened by controlling the UV exposure time to tune its modulus and probe a wide range of 3D tractions generated by a biological sample. Using this novel approach, we demonstrate that mechanomicrorobot probe can reveal distinct modulus and tractions in tumor cell colonies. Collectively, these results strongly suggest that ERMG and mechanomicrorobot can be used in a broad range of in vivo studies in mechanobiology and provide future insights into the role of mechanical factors in tumor progression and organogenesis during embryonic development."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/115946"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Erfan Mohagheghian"],"dc:subject":["Mechanobiology","Cell traction","Cell and Tissue Stiffness"],"dc:title":["Mechanoprobe fabrication and development for mechanobiology"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Mechanical Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:55Z"}