{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/115932"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/115932","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Developing oleaginous yeast as model organisms for lipid production","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01","abstract_has_math":false,"creators":["Deewan, Anshu"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemical Engineering","degree_department":null,"school":null,"contributors":["Rao, Christopher V","Kraft, Mary L","Shukla, Diwakar","Jin, Yong-Su"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-08","date_published":"2022-08","updated_at":"2026-07-22T22:24:55Z","subjects":["Oleaginous yeast","transcriptomics","RNA-seq","bioinformatics"],"languages":["en","eng"],"rights":["Copyright 2022 Anshu Deewan"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/115932","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Rao, Christopher V","Kraft, Mary L","Shukla, Diwakar","Jin, Yong-Su"]},{"key":"dc:creator","label":"Author","values":["Deewan, Anshu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-08","2022-07-15"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Oleaginous yeast","transcriptomics","RNA-seq","bioinformatics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Anshu Deewan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/115932"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01","The student, Anshu Deewan, accepted the attached license on 2022-07-13 at 10:55.","The student, Anshu Deewan, submitted this Dissertation for approval on 2022-07-13 at 10:55.","This Dissertation was approved for publication on 2022-07-15 at 11:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18280 on 2022-11-16 at 10:56:03","Over the last few decades, there has been a consistent research effort to reduce the dependence on fossil fuel-derived feedstocks. Plant biomass provides a sustainable alternative to crude-based feedstocks. Oleaginous yeasts (yeasts that can store 20% w/w lipids) are an emerging class of microorganisms, producing biofuels and bioproducts from renewable biomass. While yeasts like Rhodosporiduim toruloides, Lipomyces starkeyi, and Yarrowia lipolytica are ideal hosts for lipid production from biomass, we are limited in our understanding of the physiology of these yeasts. The work presented in this thesis aims at understanding the mechanisms involved in sugar utilization and lipid accumulation in oleaginous yeasts using various bioinformatics tools and mathematical modeling. This work improves our understanding of these yeasts and helps in the rational design of increased lipid-producing strains. Chapter 1 provides a brief introduction to the fermentation of lignocellulosic hydrolysates. Chapter 2 reports the substrate uptake and utilization pathways in the oleaginous yeast Rhodosporiduim toruloides. In Chapter 3, we report the sugar utilization pathways in Lipomyces starkeyi. Using RNA sequencing and metabolomics, we identify the genes and pathways involved in the utilization of various sugars in both the yeasts. This work was done in collaboration with Dr. Sujit Jagtap and Dr. JingJing-Liu. Chapter 4 identifies the transporters involved in sugar uptakes in yeasts and compares them to known transporters in plants. We characterize the transporter LST1_205437 from the yeast Lipomyces starkeyi, which is capable of transporting glucose and xylose simultaneously. This work was done in collaboration with Drs. Nurzhan Kuanyshev, Sujit Jagtap and JingJing-Liu. Chapter 5 discusses the insertion of a thioesterase gene in the yeast Yarrowia lipolytica for the production of medium chain alcohols. We present a bioinformatics pipeline to identify the gene's location, copy number variation, and plasmid insertion. Chapter 6 summarizes this work and discusses the next steps toward developing these yeasts as model organisms."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Developing oleaginous yeast as model organisms for lipid production"]}]}],"canonical_facts":{"dc:contributor":["Rao, Christopher V","Kraft, Mary L","Shukla, Diwakar","Jin, Yong-Su"],"dc:creator":["Deewan, Anshu"],"dc:date":["2022-08","2022-07-15"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01","The student, Anshu Deewan, accepted the attached license on 2022-07-13 at 10:55.","The student, Anshu Deewan, submitted this Dissertation for approval on 2022-07-13 at 10:55.","This Dissertation was approved for publication on 2022-07-15 at 11:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #18280 on 2022-11-16 at 10:56:03","Over the last few decades, there has been a consistent research effort to reduce the dependence on fossil fuel-derived feedstocks. Plant biomass provides a sustainable alternative to crude-based feedstocks. Oleaginous yeasts (yeasts that can store 20% w/w lipids) are an emerging class of microorganisms, producing biofuels and bioproducts from renewable biomass. While yeasts like Rhodosporiduim toruloides, Lipomyces starkeyi, and Yarrowia lipolytica are ideal hosts for lipid production from biomass, we are limited in our understanding of the physiology of these yeasts. The work presented in this thesis aims at understanding the mechanisms involved in sugar utilization and lipid accumulation in oleaginous yeasts using various bioinformatics tools and mathematical modeling. This work improves our understanding of these yeasts and helps in the rational design of increased lipid-producing strains. Chapter 1 provides a brief introduction to the fermentation of lignocellulosic hydrolysates. Chapter 2 reports the substrate uptake and utilization pathways in the oleaginous yeast Rhodosporiduim toruloides. In Chapter 3, we report the sugar utilization pathways in Lipomyces starkeyi. Using RNA sequencing and metabolomics, we identify the genes and pathways involved in the utilization of various sugars in both the yeasts. This work was done in collaboration with Dr. Sujit Jagtap and Dr. JingJing-Liu. Chapter 4 identifies the transporters involved in sugar uptakes in yeasts and compares them to known transporters in plants. We characterize the transporter LST1_205437 from the yeast Lipomyces starkeyi, which is capable of transporting glucose and xylose simultaneously. This work was done in collaboration with Drs. Nurzhan Kuanyshev, Sujit Jagtap and JingJing-Liu. Chapter 5 discusses the insertion of a thioesterase gene in the yeast Yarrowia lipolytica for the production of medium chain alcohols. We present a bioinformatics pipeline to identify the gene's location, copy number variation, and plasmid insertion. Chapter 6 summarizes this work and discusses the next steps toward developing these yeasts as model organisms."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/115932"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Anshu Deewan"],"dc:subject":["Oleaginous yeast","transcriptomics","RNA-seq","bioinformatics"],"dc:title":["Developing oleaginous yeast as model organisms for lipid production"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Chemical Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:55Z"}