{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/115829"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/115829","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Development and execution of selection methods for lysine-acylating deoxyribozymes","abstract":"This Thesis was approved for publication on 2022-04-29 at 11:20.","abstract_html":"This Thesis was approved for publication on 2022-04-29 at 11:20.","abstract_has_math":false,"creators":["Przybyla, John J (Jack)"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Silverman, Scott K"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-05","date_published":"2022-05","updated_at":"2026-07-22T22:24:55Z","subjects":["DNAzyme","Novel Catalyst","In Vitro selection","Lysine acylation","DNA catalysis"],"languages":["en","eng"],"rights":["Copyright 2022 John Przybyla"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/115829","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Silverman, Scott K"]},{"key":"dc:creator","label":"Author","values":["Przybyla, John J (Jack)"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-05","2022-04-29"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["DNAzyme","Novel Catalyst","In Vitro selection","Lysine acylation","DNA catalysis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 John Przybyla"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/115829"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This Thesis was approved for publication on 2022-04-29 at 11:20.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17973 on 2022-11-14 at 17:34:31","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-14 without embargo terms","The student, John Przybyla, accepted the attached license on 2022-04-26 at 20:37.","The student, John Przybyla, submitted this Thesis for approval on 2022-04-26 at 20:43.","DNA catalysts (deoxyribozymes, or DNAzymes) represent an exciting possibility in the world of novel catalysis. Their ability to be amplified via polymerase chain reaction (PCR) allows for in vitro selection of DNAzymes that catalyze specific reactions. Through the use of in vitro selection, DNAzymes have been identified for a growing number of reactions, including lysine side chain acylation. However, these acylating DNAzymes make use of model substrates that are attached to a complementary DNA anchor that allows for recruitment to a DNAzyme active site. These highly specific substrates do not resemble the large protein or small-molecule substrates that act as valuable targets for novel catalysts. As a result, these DNAzymes do not yet possess the level of utility that makes novel catalysts exciting. This body of work, conducted in the Silverman lab at UIUC, looks to build upon previous discoveries by establishing a selection scheme for lysine acylation in a non-anchored peptide. This includes the development of more diverse and complex substrates, the development and testing of selection conditions, and the development of a necessary “capture” step to enable the selection process. As a precursor to my ultimate project, my contributions towards the identification of amine acylating, and later lysine side chain acylating DNAzymes, will also be included. At time of writing, the selection methods discussed here are being employed in the Silverman lab to identify DNAzymes that catalyze the acylation of lysine on a non-anchored peptide. If these DNAzymes are successfully identified, they would act as another crucial step towards the identification of “useful” novel DNA catalysts."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Development and execution of selection methods for lysine-acylating deoxyribozymes"]}]}],"canonical_facts":{"dc:contributor":["Silverman, Scott K"],"dc:creator":["Przybyla, John J (Jack)"],"dc:date":["2022-05","2022-04-29"],"dc:description":["This Thesis was approved for publication on 2022-04-29 at 11:20.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17973 on 2022-11-14 at 17:34:31","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-14 without embargo terms","The student, John Przybyla, accepted the attached license on 2022-04-26 at 20:37.","The student, John Przybyla, submitted this Thesis for approval on 2022-04-26 at 20:43.","DNA catalysts (deoxyribozymes, or DNAzymes) represent an exciting possibility in the world of novel catalysis. Their ability to be amplified via polymerase chain reaction (PCR) allows for in vitro selection of DNAzymes that catalyze specific reactions. Through the use of in vitro selection, DNAzymes have been identified for a growing number of reactions, including lysine side chain acylation. However, these acylating DNAzymes make use of model substrates that are attached to a complementary DNA anchor that allows for recruitment to a DNAzyme active site. These highly specific substrates do not resemble the large protein or small-molecule substrates that act as valuable targets for novel catalysts. As a result, these DNAzymes do not yet possess the level of utility that makes novel catalysts exciting. This body of work, conducted in the Silverman lab at UIUC, looks to build upon previous discoveries by establishing a selection scheme for lysine acylation in a non-anchored peptide. This includes the development of more diverse and complex substrates, the development and testing of selection conditions, and the development of a necessary “capture” step to enable the selection process. As a precursor to my ultimate project, my contributions towards the identification of amine acylating, and later lysine side chain acylating DNAzymes, will also be included. At time of writing, the selection methods discussed here are being employed in the Silverman lab to identify DNAzymes that catalyze the acylation of lysine on a non-anchored peptide. If these DNAzymes are successfully identified, they would act as another crucial step towards the identification of “useful” novel DNA catalysts."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/115829"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 John Przybyla"],"dc:subject":["DNAzyme","Novel Catalyst","In Vitro selection","Lysine acylation","DNA catalysis"],"dc:title":["Development and execution of selection methods for lysine-acylating deoxyribozymes"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:55Z"}