{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/115724"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/115724","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Understanding peripheral and brain changes in a double hit model","abstract":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01","abstract_has_math":false,"creators":["Rymut, Haley Elizabeth"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Rodriguez-Zas, Sandra","Villamil, Maria","Caetano-Anolles, Gustavo","Wheeler, Matthew"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-05","date_published":"2022-05","updated_at":"2026-07-22T22:24:55Z","subjects":["maternal immune activation","PRRSV","cytokines","biomarkers","hippocampus","transcriptome","double hit hypothesis"],"languages":["en","eng"],"rights":["Copyright 2022 Haley Rymut"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/115724","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Rodriguez-Zas, Sandra","Villamil, Maria","Caetano-Anolles, Gustavo","Wheeler, Matthew"]},{"key":"dc:creator","label":"Author","values":["Rymut, Haley Elizabeth"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-05","2022-04-22"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["maternal immune activation","PRRSV","cytokines","biomarkers","hippocampus","transcriptome","double hit hypothesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Haley Rymut"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/115724"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01","The student, Haley Rymut, accepted the attached license on 2022-04-19 at 09:59.","The student, Haley Rymut, submitted this Dissertation for approval on 2022-04-19 at 10:06.","This Dissertation was approved for publication on 2022-04-22 at 14:16.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17790 on 2022-11-11 at 12:47:23","The combined effects of maternal immune activation (MIA), a second stress later in life, and sex have not been fully documented in pigs. Deciphering the physiological and transcriptomic effects of MIA and a second stress is especially relevant in diseases that alter fetal development. Three studies advanced the understanding of the effects of MIA and a second stress on biochemical and immune markers and gene expression in female and male pigs. In the first study, male and female pigs were weaned at 21 days of age, and the effects of weaning stress in combination with MIA were analyzed on blood indicators of organ performance and inflammation. Weaning effects on anion gap and bilirubin levels were lower among MIA pigs, suggesting a weaning stress tolerance due to MIA. The levels of cytokines interleukin 1β, interleukin 2, and interleukin 6 were highest in weaned MIA females. In the second study, blood indicators were profiled in 60-day-old pigs exposed to MIA, while the second challenge included an inflammatory challenge or fasting. While cholesterol levels suggest that prenatal MIA stress may sensitize pigs to postnatal stressors, BUN levels suggest that MIA may prime the pigs to be more tolerant to postnatal stressors. Postnatal immune challenge heightened the levels of interferon gamma and interleukin 8. The third study used RNA-sequencing to investigate the effects of weaning, MIA, and sex on gene expression in the hippocampus of 22-day old pigs. Overall, 574 genes across 17 neurological and immune pathways were differentially expressed across factor levels. The expression patterns in the terpenoid synthesis pathway indicate that MIA and weaning stress impact females more intensely. Gene over-expression was associated with MIA in neurological development pathways. Our findings offer insights into the impact of MIA and second stressors on peripheral and brain molecular profiles associated with growth and behavior."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Understanding peripheral and brain changes in a double hit model"]}]}],"canonical_facts":{"dc:contributor":["Rodriguez-Zas, Sandra","Villamil, Maria","Caetano-Anolles, Gustavo","Wheeler, Matthew"],"dc:creator":["Rymut, Haley Elizabeth"],"dc:date":["2022-05","2022-04-22"],"dc:description":["Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01","The student, Haley Rymut, accepted the attached license on 2022-04-19 at 09:59.","The student, Haley Rymut, submitted this Dissertation for approval on 2022-04-19 at 10:06.","This Dissertation was approved for publication on 2022-04-22 at 14:16.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17790 on 2022-11-11 at 12:47:23","The combined effects of maternal immune activation (MIA), a second stress later in life, and sex have not been fully documented in pigs. Deciphering the physiological and transcriptomic effects of MIA and a second stress is especially relevant in diseases that alter fetal development. Three studies advanced the understanding of the effects of MIA and a second stress on biochemical and immune markers and gene expression in female and male pigs. In the first study, male and female pigs were weaned at 21 days of age, and the effects of weaning stress in combination with MIA were analyzed on blood indicators of organ performance and inflammation. Weaning effects on anion gap and bilirubin levels were lower among MIA pigs, suggesting a weaning stress tolerance due to MIA. The levels of cytokines interleukin 1β, interleukin 2, and interleukin 6 were highest in weaned MIA females. In the second study, blood indicators were profiled in 60-day-old pigs exposed to MIA, while the second challenge included an inflammatory challenge or fasting. While cholesterol levels suggest that prenatal MIA stress may sensitize pigs to postnatal stressors, BUN levels suggest that MIA may prime the pigs to be more tolerant to postnatal stressors. Postnatal immune challenge heightened the levels of interferon gamma and interleukin 8. The third study used RNA-sequencing to investigate the effects of weaning, MIA, and sex on gene expression in the hippocampus of 22-day old pigs. Overall, 574 genes across 17 neurological and immune pathways were differentially expressed across factor levels. The expression patterns in the terpenoid synthesis pathway indicate that MIA and weaning stress impact females more intensely. Gene over-expression was associated with MIA in neurological development pathways. Our findings offer insights into the impact of MIA and second stressors on peripheral and brain molecular profiles associated with growth and behavior."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/115724"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Haley Rymut"],"dc:subject":["maternal immune activation","PRRSV","cytokines","biomarkers","hippocampus","transcriptome","double hit hypothesis"],"dc:title":["Understanding peripheral and brain changes in a double hit model"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:55Z"}