{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/115681"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/115681","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The biophysical evolution of human influenza H3N2 neuraminidase","abstract":"The student, ChuYun Teo, submitted this Thesis for approval on 2022-04-05 at 15:02.","abstract_html":"The student, ChuYun Teo, submitted this Thesis for approval on 2022-04-05 at 15:02.","abstract_has_math":false,"creators":["Teo, ChuYun"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Wu, Nicholas C"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-05","date_published":"2022-05","updated_at":"2026-07-22T22:24:55Z","subjects":["H3N2 Influenza A virus","Neuraminidase","Enzymatic activity","Stability","Surface expression."],"languages":["en","eng"],"rights":["Copyright 2022 ChuYun Teo"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/115681","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wu, Nicholas C"]},{"key":"dc:creator","label":"Author","values":["Teo, ChuYun"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-05","2022-04-08"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["H3N2 Influenza A virus","Neuraminidase","Enzymatic activity","Stability","Surface expression."]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 ChuYun Teo"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/115681"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The student, ChuYun Teo, submitted this Thesis for approval on 2022-04-05 at 15:02.","This Thesis was approved for publication on 2022-04-08 at 10:08.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17591 on 2022-11-11 at 12:18:53","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01","The student, ChuYun Teo, accepted the attached license on 2022-04-05 at 14:55.","The viral surface glycoprotein neuraminidase (NA) of influenza A virus (H3N2) has continue to evolve over the past 50 years. To date, NA has become one of the vaccine targets and the evolution of NA in escaping immune response is rarely explored. This study aimed towards understanding the biophysical properties of different human H3N2 NA strains that were isolated across 50 years. This study employed biochemical assay to detect NA expression, cell surface activity, protein activity, and stability. Our analysis showed that NA with higher surface expression has stronger cell surface enzyme activity. In addition, protein stability had no effect on the recombinant NA enzymatic activity. Overall, these results provide a mechanistic understanding of NA evolution of human H3N2 influenza A virus."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The biophysical evolution of human influenza H3N2 neuraminidase"]}]}],"canonical_facts":{"dc:contributor":["Wu, Nicholas C"],"dc:creator":["Teo, ChuYun"],"dc:date":["2022-05","2022-04-08"],"dc:description":["The student, ChuYun Teo, submitted this Thesis for approval on 2022-04-05 at 15:02.","This Thesis was approved for publication on 2022-04-08 at 10:08.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17591 on 2022-11-11 at 12:18:53","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01","The student, ChuYun Teo, accepted the attached license on 2022-04-05 at 14:55.","The viral surface glycoprotein neuraminidase (NA) of influenza A virus (H3N2) has continue to evolve over the past 50 years. To date, NA has become one of the vaccine targets and the evolution of NA in escaping immune response is rarely explored. This study aimed towards understanding the biophysical properties of different human H3N2 NA strains that were isolated across 50 years. This study employed biochemical assay to detect NA expression, cell surface activity, protein activity, and stability. Our analysis showed that NA with higher surface expression has stronger cell surface enzyme activity. In addition, protein stability had no effect on the recombinant NA enzymatic activity. Overall, these results provide a mechanistic understanding of NA evolution of human H3N2 influenza A virus."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/115681"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 ChuYun Teo"],"dc:subject":["H3N2 Influenza A virus","Neuraminidase","Enzymatic activity","Stability","Surface expression."],"dc:title":["The biophysical evolution of human influenza H3N2 neuraminidase"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:55Z"}