{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/115593"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/115593","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Mechanisms and applications of anti-phage defenses in staphylococci","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01","abstract_has_math":false,"creators":["Bari, S M Nayeemul"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Hatoum-Aslan, Asma","Whitaker , Rachel","Brooke, Christopher","Dokland, Terje"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-05","date_published":"2022-05","updated_at":"2026-07-22T22:24:54Z","subjects":["Stphylococci","Bacteriophage","Phage therapy","Anti-phage defense","CRISPR-Cas"],"languages":["en","eng"],"rights":["Copyright 2022 S M Nayeemul Bari"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/115593","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hatoum-Aslan, Asma","Whitaker , Rachel","Brooke, Christopher","Dokland, Terje"]},{"key":"dc:creator","label":"Author","values":["Bari, S M Nayeemul"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-05","2022-04-22"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Stphylococci","Bacteriophage","Phage therapy","Anti-phage defense","CRISPR-Cas"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 S M Nayeemul Bari"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/115593"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01","The student, S M Nayeemul Bari, accepted the attached license on 2022-04-21 at 00:21.","The student, S M Nayeemul Bari, submitted this Dissertation for approval on 2022-04-21 at 00:21.","This Dissertation was approved for publication on 2022-04-22 at 09:02.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17870 on 2022-11-11 at 12:11:42","Staphylococci are skin-dwelling bacteria that cause antibiotic-resistant nosocomial infections. Bacteriophages (or phages for short) are the viral predators of bacteria and can infect and destroy their hosts within minutes of infection. This extraordinary property makes phages promising alternatives to conventional antibiotics for the treatment of drug-resistant infections. However, the successful implementation of phage therapy is confronted by several challenges. First, phages carry an inherent risk of undesirable side effects because their genomes encode many genes with unknown functions, and their molecular interactions with the bacterial hosts are poorly understood. Secondly, bacteria harbor a variety of anti-phage immune systems which threaten to undermine the effectiveness of whole-phage therapies. Therefore, gaining a better understanding of staphylococcal phages and their molecular interactions with the bacterial host is extremely important in order to develop safe and effective phage-based antimicrobials. The research described herein addresses these challenges. We have developed strategies to genetically engineer staphylococcal phages using the adaptive immune system known as CRISPR-Cas. We also discovered a novel anti-phage defense system mediated by a single multifunctional enzyme with nuclease and helicase functions. This enzyme provides robust immunity against diverse families of staphylococcal phages by targeting their nucleic acids. Knowledge gained from our findings is expected to enable the engineering of safe and effective phage-based antimicrobials that eliminate staphylococcal infections."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Mechanisms and applications of anti-phage defenses in staphylococci"]}]}],"canonical_facts":{"dc:contributor":["Hatoum-Aslan, Asma","Whitaker , Rachel","Brooke, Christopher","Dokland, Terje"],"dc:creator":["Bari, S M Nayeemul"],"dc:date":["2022-05","2022-04-22"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01","The student, S M Nayeemul Bari, accepted the attached license on 2022-04-21 at 00:21.","The student, S M Nayeemul Bari, submitted this Dissertation for approval on 2022-04-21 at 00:21.","This Dissertation was approved for publication on 2022-04-22 at 09:02.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17870 on 2022-11-11 at 12:11:42","Staphylococci are skin-dwelling bacteria that cause antibiotic-resistant nosocomial infections. Bacteriophages (or phages for short) are the viral predators of bacteria and can infect and destroy their hosts within minutes of infection. This extraordinary property makes phages promising alternatives to conventional antibiotics for the treatment of drug-resistant infections. However, the successful implementation of phage therapy is confronted by several challenges. First, phages carry an inherent risk of undesirable side effects because their genomes encode many genes with unknown functions, and their molecular interactions with the bacterial hosts are poorly understood. Secondly, bacteria harbor a variety of anti-phage immune systems which threaten to undermine the effectiveness of whole-phage therapies. Therefore, gaining a better understanding of staphylococcal phages and their molecular interactions with the bacterial host is extremely important in order to develop safe and effective phage-based antimicrobials. The research described herein addresses these challenges. We have developed strategies to genetically engineer staphylococcal phages using the adaptive immune system known as CRISPR-Cas. We also discovered a novel anti-phage defense system mediated by a single multifunctional enzyme with nuclease and helicase functions. This enzyme provides robust immunity against diverse families of staphylococcal phages by targeting their nucleic acids. Knowledge gained from our findings is expected to enable the engineering of safe and effective phage-based antimicrobials that eliminate staphylococcal infections."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/115593"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 S M Nayeemul Bari"],"dc:subject":["Stphylococci","Bacteriophage","Phage therapy","Anti-phage defense","CRISPR-Cas"],"dc:title":["Mechanisms and applications of anti-phage defenses in staphylococci"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:54Z"}