{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/115519"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/115519","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of endogenous D-amino acids in mammals via chiral separations","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01","abstract_has_math":false,"creators":["Lee, Cindy Jung"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Sweedler, Jonathan V","Dobrucki, Wawrzyniec","Gewirth, Andrew A","Rodríguez-López, Joaquín"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-05","date_published":"2022-05","updated_at":"2026-07-22T22:24:54Z","subjects":["D-amino acids","Chiral Separations","Capillary Electrophoresis","Liquid Chromatography","Mass Spectrometry"],"languages":["en","eng"],"rights":["Copyright 2022 Cindy Jung Lee"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/115519","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sweedler, Jonathan V","Dobrucki, Wawrzyniec","Gewirth, Andrew A","Rodríguez-López, Joaquín"]},{"key":"dc:creator","label":"Author","values":["Lee, Cindy Jung"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-05","2022-04-08"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["D-amino acids","Chiral Separations","Capillary Electrophoresis","Liquid Chromatography","Mass Spectrometry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Cindy Jung Lee"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/115519"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01","The student, Cindy Lee, accepted the attached license on 2022-03-31 at 15:59.","The student, Cindy Lee, submitted this Dissertation for approval on 2022-03-31 at 16:24.","This Dissertation was approved for publication on 2022-04-08 at 08:45.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17568 on 2022-11-11 at 11:55:53","Amino acids are one of the essential molecules for living organisms. Not only are amino acids required for vital processes such as building proteins and peptides, but they are also involved in cell-to-cell signaling. For a long time, it was believed that only L-amino acids were physiologically relevant molecules in mammals. However, diverse D-amino acids, the enantiomers of L-amino acids, have been detected in mammals with the aid of analytical advancements, and their biological significance, which differs from their L-enantiomers due to the chirality, has been studied. The distribution, origin, physiological relevance, and disease implications of D-amino acids have been gradually elucidated. For example, D-amino acids, like D-serine and D-aspartate, have been gaining research interest for their involvement in learning and memory. As a result, many current studies on D-amino acids are largely limited to the central nervous system. The knowledge of endogenous D-amino acids beyond the central nervous system, therefore, remains a mystery and requires further study to establish their metabolic pathways and functions. Nevertheless, studying the physiological and pathological function of D-amino acids in mammals requires sensitive and selective analytical determination of these low abundant and heterogeneously distributed molecules, while distinguishing them from higher concentrations of L-enantiomers based on chirality, in complex biological systems. Here, chiral separation techniques involving capillary electrophoresis-laser induced fluorescence and liquid chromatography-tandem mass spectrometry with methodological optimization are described to improve the quantitative characterization of D-amino acids in various tissues of mammals, including humans. Specifically, D-amino acids in cochlea, gut, more precisely gut microbiota, pancreatic islets, and serum are delineated to advance our understanding of these enigmatic cell-to-cell signaling molecules beyond the central nervous system."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Characterization of endogenous D-amino acids in mammals via chiral separations"]}]}],"canonical_facts":{"dc:contributor":["Sweedler, Jonathan V","Dobrucki, Wawrzyniec","Gewirth, Andrew A","Rodríguez-López, Joaquín"],"dc:creator":["Lee, Cindy Jung"],"dc:date":["2022-05","2022-04-08"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2024-05-01","The student, Cindy Lee, accepted the attached license on 2022-03-31 at 15:59.","The student, Cindy Lee, submitted this Dissertation for approval on 2022-03-31 at 16:24.","This Dissertation was approved for publication on 2022-04-08 at 08:45.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17568 on 2022-11-11 at 11:55:53","Amino acids are one of the essential molecules for living organisms. Not only are amino acids required for vital processes such as building proteins and peptides, but they are also involved in cell-to-cell signaling. For a long time, it was believed that only L-amino acids were physiologically relevant molecules in mammals. However, diverse D-amino acids, the enantiomers of L-amino acids, have been detected in mammals with the aid of analytical advancements, and their biological significance, which differs from their L-enantiomers due to the chirality, has been studied. The distribution, origin, physiological relevance, and disease implications of D-amino acids have been gradually elucidated. For example, D-amino acids, like D-serine and D-aspartate, have been gaining research interest for their involvement in learning and memory. As a result, many current studies on D-amino acids are largely limited to the central nervous system. The knowledge of endogenous D-amino acids beyond the central nervous system, therefore, remains a mystery and requires further study to establish their metabolic pathways and functions. Nevertheless, studying the physiological and pathological function of D-amino acids in mammals requires sensitive and selective analytical determination of these low abundant and heterogeneously distributed molecules, while distinguishing them from higher concentrations of L-enantiomers based on chirality, in complex biological systems. Here, chiral separation techniques involving capillary electrophoresis-laser induced fluorescence and liquid chromatography-tandem mass spectrometry with methodological optimization are described to improve the quantitative characterization of D-amino acids in various tissues of mammals, including humans. Specifically, D-amino acids in cochlea, gut, more precisely gut microbiota, pancreatic islets, and serum are delineated to advance our understanding of these enigmatic cell-to-cell signaling molecules beyond the central nervous system."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/115519"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Cindy Jung Lee"],"dc:subject":["D-amino acids","Chiral Separations","Capillary Electrophoresis","Liquid Chromatography","Mass Spectrometry"],"dc:title":["Characterization of endogenous D-amino acids in mammals via chiral separations"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:54Z"}