{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/115420"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/115420","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Spatially-defined quantitative interleukin 6 (IL-6) detection using polyethylene glycol microgel sensors (μGELISA)","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms","abstract_has_math":false,"creators":["Ryoo, Hyeon"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Underhill, Gregory H"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-05","date_published":"2022-05","updated_at":"2026-07-22T22:24:54Z","subjects":["Microgel","Sensor","Granular hydrogel","IL-6","3D Cell Culture","Polyethylene glycol","Hepatic stellate cell","Microwell","Protein sensor","ELISA"],"languages":["en","eng"],"rights":["Copyright 2022 Hyeon Ryoo"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/115420","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Underhill, Gregory H"]},{"key":"dc:creator","label":"Author","values":["Ryoo, Hyeon"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-05","2022-04-25"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microgel","Sensor","Granular hydrogel","IL-6","3D Cell Culture","Polyethylene glycol","Hepatic stellate cell","Microwell","Protein sensor","ELISA"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2022 Hyeon Ryoo"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/115420"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms","The student, Hyeon Ryoo, accepted the attached license on 2022-04-21 at 12:02.","The student, Hyeon Ryoo, submitted this Thesis for approval on 2022-04-21 at 12:11.","This Thesis was approved for publication on 2022-04-25 at 16:24.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17879 on 2022-11-11 at 13:42:45","Cells communicate with each other in multiple ways, with proteins as one of these signals. IL-6 is a pleiotropic cytokine involved in inflammation. IL-6 has a seemingly double-sided effect in liver pathologies, enhancing liver steatosis but also reducing liver injury. This ambiguous nature makes it imperative to find a research tool that can quantify local IL-6 concentrations in in vitro cell culture. By optimizing an ELISA system fitted into polyethylene glycol (PEG) microgels, we have developed an easy-to-use IL-6 sensor enabled for 3D cell culture systems. Using a confocal microscope, the microgels are able to detect IL-6 concentrations from 2.95 ng/mL and 33.56 ng/mL (middle 80% detection range). This concentration range fit well within the IL-6 release profile of small HSC aggregates, allowing us to quantify the exact IL-6 concentration starting at an average minimum distance of 14.68 μm from the aggregate. The μGELISA system could be adapted to proteins other than IL-6 by modifying the antibody sets used for capture and detection. Our μGELISA system could also be used to study cell-to-cell communication through protein signals and the effect these local concentrations have on each other, all within a closed-loop environment."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Spatially-defined quantitative interleukin 6 (IL-6) detection using polyethylene glycol microgel sensors (μGELISA)"]}]}],"canonical_facts":{"dc:contributor":["Underhill, Gregory H"],"dc:creator":["Ryoo, Hyeon"],"dc:date":["2022-05","2022-04-25"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-11 without embargo terms","The student, Hyeon Ryoo, accepted the attached license on 2022-04-21 at 12:02.","The student, Hyeon Ryoo, submitted this Thesis for approval on 2022-04-21 at 12:11.","This Thesis was approved for publication on 2022-04-25 at 16:24.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17879 on 2022-11-11 at 13:42:45","Cells communicate with each other in multiple ways, with proteins as one of these signals. IL-6 is a pleiotropic cytokine involved in inflammation. IL-6 has a seemingly double-sided effect in liver pathologies, enhancing liver steatosis but also reducing liver injury. This ambiguous nature makes it imperative to find a research tool that can quantify local IL-6 concentrations in in vitro cell culture. By optimizing an ELISA system fitted into polyethylene glycol (PEG) microgels, we have developed an easy-to-use IL-6 sensor enabled for 3D cell culture systems. Using a confocal microscope, the microgels are able to detect IL-6 concentrations from 2.95 ng/mL and 33.56 ng/mL (middle 80% detection range). This concentration range fit well within the IL-6 release profile of small HSC aggregates, allowing us to quantify the exact IL-6 concentration starting at an average minimum distance of 14.68 μm from the aggregate. The μGELISA system could be adapted to proteins other than IL-6 by modifying the antibody sets used for capture and detection. Our μGELISA system could also be used to study cell-to-cell communication through protein signals and the effect these local concentrations have on each other, all within a closed-loop environment."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/115420"],"dc:language":["en","eng"],"dc:rights":["Copyright 2022 Hyeon Ryoo"],"dc:subject":["Microgel","Sensor","Granular hydrogel","IL-6","3D Cell Culture","Polyethylene glycol","Hepatic stellate cell","Microwell","Protein sensor","ELISA"],"dc:title":["Spatially-defined quantitative interleukin 6 (IL-6) detection using polyethylene glycol microgel sensors (μGELISA)"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:54Z"}