{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/114028"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/114028","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"SARS-CoV-2 detection in wastewater","abstract":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01","abstract_html":"Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01","abstract_has_math":false,"creators":["Zhou, Aijia"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Environ Engr in Civil Engr","degree_department":null,"school":null,"contributors":["Nguyen, Thanh Huong"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-04-29T21:47:48Z","date_published":"2022-04-29T21:47:48Z","updated_at":"2026-07-22T22:24:54Z","subjects":["Environmental engineering"],"languages":["en","eng"],"rights":["Copyright 2021 Aijia Zhou"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/114028","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Nguyen, Thanh Huong"]},{"key":"dc:creator","label":"Author","values":["Zhou, Aijia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-04-29T21:47:48Z","2024-04-29T21:47:53Z","2021-12","2021-12-10"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environ Engr in Civil Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Environmental engineering"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Aijia Zhou"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/114028"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01","The student, Aijia Zhou, accepted the attached license on 2021-12-09 at 16:09.","The student, Aijia Zhou, submitted this Thesis for approval on 2021-12-09 at 16:16.","This Thesis was approved for publication on 2021-12-10 at 13:35.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17439 on 2022-04-06 at 17:18:06","Made available in DSpace on 2022-04-29T21:47:48Z (GMT). No. of bitstreams: 3 ZHOU-THESIS-2021.pdf: 1872344 bytes, checksum: c06f40ef267430c12df848301b7cffa8 (MD5) Supplemental Infomation.xlsx: 35397 bytes, checksum: b339f415b75d9442a5efd5764844eddc (MD5) LICENSE.txt: 4207 bytes, checksum: c80e541f4e52f96a21674546781a2cd5 (MD5) Previous issue date: 2021-12-10","Embargo set by: Seth Robbins for item 123393 Lift date: 2024-04-29T21:47:53Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only","COVID-19 pandemic is a global concern which has caused millions of cases and deaths. Wastewater-based epidemiology (WBE) can track SARS-CoV-2 infection dynamics through wastewater. Aiming to detect the existence of pre-symptomatic or asymptomatic patients in specific areas prior clinical test results, and to remind special attention for viral spreading, the SARS-CoV-2 viral concentration in wastewater was monitored from April to October 2021.Wastewater samples were collected in 9 locations in Urbana-Champaign and a rural area nearby. Our study developed a timesaving WBE method, which used MgCl2 addition and centrifugation to concentrate SARS-CoV-2 into sludge, based on the understanding that enveloped virus RNA in sludge was dozens of times higher than it in supernatant. We correlated wastewater viral concentration data with clinical tests and found Pearson’s relationship showed weak to moderate positive in four of seven sampling locations. Campus town, the location where had most frequent clinical tests, performed the best correlation. In addition, it was found the appearance of undetectable RNA concentration overlapped with zero clinical cases. The infection peaks of reporting cases always occurred in the periods when viral concentration kept stable."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["SARS-CoV-2 detection in wastewater"]}]}],"canonical_facts":{"dc:contributor":["Nguyen, Thanh Huong"],"dc:creator":["Zhou, Aijia"],"dc:date":["2022-04-29T21:47:48Z","2024-04-29T21:47:53Z","2021-12","2021-12-10"],"dc:description":["Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01","The student, Aijia Zhou, accepted the attached license on 2021-12-09 at 16:09.","The student, Aijia Zhou, submitted this Thesis for approval on 2021-12-09 at 16:16.","This Thesis was approved for publication on 2021-12-10 at 13:35.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17439 on 2022-04-06 at 17:18:06","Made available in DSpace on 2022-04-29T21:47:48Z (GMT). No. of bitstreams: 3 ZHOU-THESIS-2021.pdf: 1872344 bytes, checksum: c06f40ef267430c12df848301b7cffa8 (MD5) Supplemental Infomation.xlsx: 35397 bytes, checksum: b339f415b75d9442a5efd5764844eddc (MD5) LICENSE.txt: 4207 bytes, checksum: c80e541f4e52f96a21674546781a2cd5 (MD5) Previous issue date: 2021-12-10","Embargo set by: Seth Robbins for item 123393 Lift date: 2024-04-29T21:47:53Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only","COVID-19 pandemic is a global concern which has caused millions of cases and deaths. Wastewater-based epidemiology (WBE) can track SARS-CoV-2 infection dynamics through wastewater. Aiming to detect the existence of pre-symptomatic or asymptomatic patients in specific areas prior clinical test results, and to remind special attention for viral spreading, the SARS-CoV-2 viral concentration in wastewater was monitored from April to October 2021.Wastewater samples were collected in 9 locations in Urbana-Champaign and a rural area nearby. Our study developed a timesaving WBE method, which used MgCl2 addition and centrifugation to concentrate SARS-CoV-2 into sludge, based on the understanding that enveloped virus RNA in sludge was dozens of times higher than it in supernatant. We correlated wastewater viral concentration data with clinical tests and found Pearson’s relationship showed weak to moderate positive in four of seven sampling locations. Campus town, the location where had most frequent clinical tests, performed the best correlation. In addition, it was found the appearance of undetectable RNA concentration overlapped with zero clinical cases. The infection peaks of reporting cases always occurred in the periods when viral concentration kept stable."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/114028"],"dc:language":["en","eng"],"dc:rights":["Copyright 2021 Aijia Zhou"],"dc:subject":["Environmental engineering"],"dc:title":["SARS-CoV-2 detection in wastewater"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Environ Engr in Civil Engr"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:54Z"}