{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/113917"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/113917","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Increased levels of ploidy in A. texanum-dependent A. platineum (JJL) populations in eastern Illinois: conservation implications for the state endangered species","abstract":"The silvery salamander, Ambystoma platineum, listed as endangered in Illinois, originated from the hybridization between Ambystoma jeffersonianum and Ambystoma laterale. A. platineum are triploid (JJL) and usually reproduce gynogenetically using one of the parental species as a sexual host. There is a population of A. platineum at Kickapoo State Recreation Area (KSRA), Vermillion County, Illinois that uses Ambystoma texanum as a sexual host instead and has had persistent levels of tetraploidy. This has been shown to be a result of the fertilization of unreduced A. platinuem ova by Ambystoma texanum sperm (JJLTx). More recently at least 6 other A. texanum-dependent A. platineum populations have been discovered in Vermillion and Crawford Counties, Illinois. The purpose of this study was to answer the conservation question: is hybridization in A. texanum-dependent A. platineum populations in Illinois posing a threat to the State endangered A. platineum by creating tetraploid salamanders between some A. texanum X A. platineum crosses? A. platineum tissue was collected at 11 wetlands. A. texanum specific microsatellite primers were run on the extracted tissue DNA to differentiate triploid from tetraploid salamanders between ponds and across sites. This is the first study that has looked at levels of tetraploidy across ponds at several locations in Illinois and is the first to compile longitudinal ploidy data over a span of 41 years at a single wetland (KSRA 283). Although tetraploids (JJLTx) are found at all wetlands, the relatively low levels suggest that JJLTx are not threatening the viability of triploid A. platineum. In addition, these results will provide an understanding of the distribution and abundance of tetraploids in east central Illinois, allowing for development of better strategies to protect this state endangered species.","abstract_html":"The silvery salamander, Ambystoma platineum, listed as endangered in Illinois, originated from the hybridization between Ambystoma jeffersonianum and Ambystoma laterale. A. platineum are triploid (JJL) and usually reproduce gynogenetically using one of the parental species as a sexual host. There is a population of A. platineum at Kickapoo State Recreation Area (KSRA), Vermillion County, Illinois that uses Ambystoma texanum as a sexual host instead and has had persistent levels of tetraploidy. This has been shown to be a result of the fertilization of unreduced A. platinuem ova by Ambystoma texanum sperm (JJLTx). More recently at least 6 other A. texanum-dependent A. platineum populations have been discovered in Vermillion and Crawford Counties, Illinois. The purpose of this study was to answer the conservation question: is hybridization in A. texanum-dependent A. platineum populations in Illinois posing a threat to the State endangered A. platineum by creating tetraploid salamanders between some A. texanum X A. platineum crosses? A. platineum tissue was collected at 11 wetlands. A. texanum specific microsatellite primers were run on the extracted tissue DNA to differentiate triploid from tetraploid salamanders between ponds and across sites. This is the first study that has looked at levels of tetraploidy across ponds at several locations in Illinois and is the first to compile longitudinal ploidy data over a span of 41 years at a single wetland (KSRA 283). Although tetraploids (JJLTx) are found at all wetlands, the relatively low levels suggest that JJLTx are not threatening the viability of triploid A. platineum. In addition, these results will provide an understanding of the distribution and abundance of tetraploids in east central Illinois, allowing for development of better strategies to protect this state endangered species.","abstract_has_math":false,"creators":["Laserna Cowal, Victoria Alessa"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Paige, Ken N","Phillips, Chris A","Kuhns, Andrew R"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-04-29T21:34:53Z","date_published":"2022-04-29T21:34:53Z","updated_at":"2026-07-22T22:24:53Z","subjects":["Unisexual","conservation","Ambystoma","polyploidy"],"languages":["en"],"rights":["Copyright 2021 Victoria Laserna Cowal"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/113917","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Paige, Ken N","Phillips, Chris A","Kuhns, Andrew R"]},{"key":"dc:creator","label":"Author","values":["Laserna Cowal, Victoria Alessa"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-04-29T21:34:53Z","2021-12-06","2021-12"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Unisexual","conservation","Ambystoma","polyploidy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Victoria Laserna Cowal"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/113917"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The silvery salamander, Ambystoma platineum, listed as endangered in Illinois, originated from the hybridization between Ambystoma jeffersonianum and Ambystoma laterale. A. platineum are triploid (JJL) and usually reproduce gynogenetically using one of the parental species as a sexual host. There is a population of A. platineum at Kickapoo State Recreation Area (KSRA), Vermillion County, Illinois that uses Ambystoma texanum as a sexual host instead and has had persistent levels of tetraploidy. This has been shown to be a result of the fertilization of unreduced A. platinuem ova by Ambystoma texanum sperm (JJLTx). More recently at least 6 other A. texanum-dependent A. platineum populations have been discovered in Vermillion and Crawford Counties, Illinois. The purpose of this study was to answer the conservation question: is hybridization in A. texanum-dependent A. platineum populations in Illinois posing a threat to the State endangered A. platineum by creating tetraploid salamanders between some A. texanum X A. platineum crosses? A. platineum tissue was collected at 11 wetlands. A. texanum specific microsatellite primers were run on the extracted tissue DNA to differentiate triploid from tetraploid salamanders between ponds and across sites. This is the first study that has looked at levels of tetraploidy across ponds at several locations in Illinois and is the first to compile longitudinal ploidy data over a span of 41 years at a single wetland (KSRA 283). Although tetraploids (JJLTx) are found at all wetlands, the relatively low levels suggest that JJLTx are not threatening the viability of triploid A. platineum. In addition, these results will provide an understanding of the distribution and abundance of tetraploids in east central Illinois, allowing for development of better strategies to protect this state endangered species.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-04-06 without embargo terms","The student, Victoria Laserna Cowal, accepted the attached license on 2021-12-03 at 13:07.","The student, Victoria Laserna Cowal, submitted this Thesis for approval on 2021-12-03 at 13:21.","This Thesis was approved for publication on 2021-12-06 at 13:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17384 on 2022-04-06 at 17:11:02","Made available in DSpace on 2022-04-29T21:34:53Z (GMT). No. of bitstreams: 2 LASERNACOWAL-THESIS-2021.pdf: 392372 bytes, checksum: 279ede210fd78b8b37bc5f1896202630 (MD5) LICENSE.txt: 4217 bytes, checksum: baecc1158ec28234b3b67c042ecc6a15 (MD5) Previous issue date: 2021-12-06"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Increased levels of ploidy in A. texanum-dependent A. platineum (JJL) populations in eastern Illinois: conservation implications for the state endangered species"]}]}],"canonical_facts":{"dc:contributor":["Paige, Ken N","Phillips, Chris A","Kuhns, Andrew R"],"dc:creator":["Laserna Cowal, Victoria Alessa"],"dc:date":["2022-04-29T21:34:53Z","2021-12-06","2021-12"],"dc:description":["The silvery salamander, Ambystoma platineum, listed as endangered in Illinois, originated from the hybridization between Ambystoma jeffersonianum and Ambystoma laterale. A. platineum are triploid (JJL) and usually reproduce gynogenetically using one of the parental species as a sexual host. There is a population of A. platineum at Kickapoo State Recreation Area (KSRA), Vermillion County, Illinois that uses Ambystoma texanum as a sexual host instead and has had persistent levels of tetraploidy. This has been shown to be a result of the fertilization of unreduced A. platinuem ova by Ambystoma texanum sperm (JJLTx). More recently at least 6 other A. texanum-dependent A. platineum populations have been discovered in Vermillion and Crawford Counties, Illinois. The purpose of this study was to answer the conservation question: is hybridization in A. texanum-dependent A. platineum populations in Illinois posing a threat to the State endangered A. platineum by creating tetraploid salamanders between some A. texanum X A. platineum crosses? A. platineum tissue was collected at 11 wetlands. A. texanum specific microsatellite primers were run on the extracted tissue DNA to differentiate triploid from tetraploid salamanders between ponds and across sites. This is the first study that has looked at levels of tetraploidy across ponds at several locations in Illinois and is the first to compile longitudinal ploidy data over a span of 41 years at a single wetland (KSRA 283). Although tetraploids (JJLTx) are found at all wetlands, the relatively low levels suggest that JJLTx are not threatening the viability of triploid A. platineum. In addition, these results will provide an understanding of the distribution and abundance of tetraploids in east central Illinois, allowing for development of better strategies to protect this state endangered species.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-04-06 without embargo terms","The student, Victoria Laserna Cowal, accepted the attached license on 2021-12-03 at 13:07.","The student, Victoria Laserna Cowal, submitted this Thesis for approval on 2021-12-03 at 13:21.","This Thesis was approved for publication on 2021-12-06 at 13:10.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17384 on 2022-04-06 at 17:11:02","Made available in DSpace on 2022-04-29T21:34:53Z (GMT). No. of bitstreams: 2 LASERNACOWAL-THESIS-2021.pdf: 392372 bytes, checksum: 279ede210fd78b8b37bc5f1896202630 (MD5) LICENSE.txt: 4217 bytes, checksum: baecc1158ec28234b3b67c042ecc6a15 (MD5) Previous issue date: 2021-12-06"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/113917"],"dc:language":["en"],"dc:rights":["Copyright 2021 Victoria Laserna Cowal"],"dc:subject":["Unisexual","conservation","Ambystoma","polyploidy"],"dc:title":["Increased levels of ploidy in A. texanum-dependent A. platineum (JJL) populations in eastern Illinois: conservation implications for the state endangered species"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:53Z"}