{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/113353"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/113353","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Evaluation of Escherichia coli O157:H7 and Salmonella enterica inactivation in water by several treatment technologies","abstract":"Water treatment technologies are necessary for microbial pathogen removal from potable water. Human activities, including large industries in agriculture and manufacturing, produce massive amounts of waste that invariably end up in water supplies. There will always be a need for water treatment technologies that evolve and progress as waste volume and complexity increases. Further exploration and development of current treatment methods will be necessary to match this demand. In this study, wintergreen essential oil, physical filtration, iodine and chlorine dioxide tablets, sunlight, artificial UV light, and TiO2 photocatalyst treatment methods and their effects on waterborne pathogens Escherichia coli O157:H7 and Salmonella enterica are evaluated. The effect of wintergreen essential oil derived from Gaultheria procumbens was analyzed against E. coli and S. enterica in concentrations of 5 μL/1.5 mL, 2.5 μL/1.5 mL, and 1 μL/1.5 mL (wintergreen/sterilized deionized water). Experiments of UV lamp exposure, with and without immobilized TiO2 photocatalyst, were performed to determine pathogen inactivation efficiency. Sunlight alone and with immobilized TiO2 photocatalyst were studied for pathogen inactivation potential as well. Lastly, physical filtration via portable backpacking pump filter and purification by emergency chlorine dioxide and iodine tablets were tested for removal and purification efficiencies against E. coli and S. enterica. Bacterial viability was quantified by serial dilution and viable plate counting. Preliminary experiments were also done to develop methods and procedures, including the effect of refrigerated storage on E. coli viability in Tryptic Soy Broth and the development of E. coli and S. enterica growth curves by optical density at 600 nm (OD 600 ) with spectrophotometry measurements. Results of these experiments are discussed and compared. Statistical analysis via one-way ANOVA with a post-hoc Tukey HSD was used to analyze difference in treatments for significance. Results showed pathogen removal for all technologies studied, to varying degrees. Some examples include: 5-log reduction was achieved in E. coli with sunlight-only at 6 hours; 3-log reduction was achieved in S. enterica with sunlight-only at 7.5 hours, E. coli UV lamp-only at 8 hours, E. coli and S. enterica with sunlight/TiO2 at 6 and 7.5 hours, respectively, and E. coli with a 5 μL concentration of Wintergreen essential oil at 50 minutes; 3-log reduction in E. coli was observed when the 5 μL concentration of wintergreen essential oil was applied for 50 minutes, 2-log reduction in E. coli was observed after 50 minutes of 2.5 μL concentration treatment, while the 1 μL concentration of wintergreen essential oil achieved only a 40% reduction in E. coli at 50 minutes. The results from this research may influence further pursuit in one or combinations of these water treatment technologies.","abstract_html":"Water treatment technologies are necessary for microbial pathogen removal from potable water. Human activities, including large industries in agriculture and manufacturing, produce massive amounts of waste that invariably end up in water supplies. There will always be a need for water treatment technologies that evolve and progress as waste volume and complexity increases. Further exploration and development of current treatment methods will be necessary to match this demand. In this study, wintergreen essential oil, physical filtration, iodine and chlorine dioxide tablets, sunlight, artificial UV light, and TiO2 photocatalyst treatment methods and their effects on waterborne pathogens Escherichia coli O157:H7 and Salmonella enterica are evaluated. The effect of wintergreen essential oil derived from Gaultheria procumbens was analyzed against E. coli and S. enterica in concentrations of 5 μL/1.5 mL, 2.5 μL/1.5 mL, and 1 μL/1.5 mL (wintergreen/sterilized deionized water). Experiments of UV lamp exposure, with and without immobilized TiO2 photocatalyst, were performed to determine pathogen inactivation efficiency. Sunlight alone and with immobilized TiO2 photocatalyst were studied for pathogen inactivation potential as well. Lastly, physical filtration via portable backpacking pump filter and purification by emergency chlorine dioxide and iodine tablets were tested for removal and purification efficiencies against E. coli and S. enterica. Bacterial viability was quantified by serial dilution and viable plate counting. Preliminary experiments were also done to develop methods and procedures, including the effect of refrigerated storage on E. coli viability in Tryptic Soy Broth and the development of E. coli and S. enterica growth curves by optical density at 600 nm (OD 600 ) with spectrophotometry measurements. Results of these experiments are discussed and compared. Statistical analysis via one-way ANOVA with a post-hoc Tukey HSD was used to analyze difference in treatments for significance. Results showed pathogen removal for all technologies studied, to varying degrees. Some examples include: 5-log reduction was achieved in E. coli with sunlight-only at 6 hours; 3-log reduction was achieved in S. enterica with sunlight-only at 7.5 hours, E. coli UV lamp-only at 8 hours, E. coli and S. enterica with sunlight/TiO2 at 6 and 7.5 hours, respectively, and E. coli with a 5 μL concentration of Wintergreen essential oil at 50 minutes; 3-log reduction in E. coli was observed when the 5 μL concentration of wintergreen essential oil was applied for 50 minutes, 2-log reduction in E. coli was observed after 50 minutes of 2.5 μL concentration treatment, while the 1 μL concentration of wintergreen essential oil achieved only a 40% reduction in E. coli at 50 minutes. The results from this research may influence further pursuit in one or combinations of these water treatment technologies.","abstract_has_math":false,"creators":["Miller, Matthew Robert"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Agricultural & Biological Engr","degree_department":null,"school":null,"contributors":["Davidson, Paul C","Kalita, Prasanta K","Bhattarai, Rabin"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-01-12T22:56:16Z","date_published":"2022-01-12T22:56:16Z","updated_at":"2026-07-22T22:24:53Z","subjects":["Escherichia coli O157:H7","Salmonella enterica"],"languages":["en"],"rights":["Copyright 2021 Matthew Miller"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/113353","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Davidson, Paul C","Kalita, Prasanta K","Bhattarai, Rabin"]},{"key":"dc:creator","label":"Author","values":["Miller, Matthew Robert"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2022-01-12T22:56:16Z","2024-01-12T22:56:20Z","2021-07-23","2021-08"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Agricultural & Biological Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Escherichia coli O157:H7","Salmonella enterica"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Matthew Miller"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/113353"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Water treatment technologies are necessary for microbial pathogen removal from potable water. Human activities, including large industries in agriculture and manufacturing, produce massive amounts of waste that invariably end up in water supplies. There will always be a need for water treatment technologies that evolve and progress as waste volume and complexity increases. Further exploration and development of current treatment methods will be necessary to match this demand. In this study, wintergreen essential oil, physical filtration, iodine and chlorine dioxide tablets, sunlight, artificial UV light, and TiO2 photocatalyst treatment methods and their effects on waterborne pathogens Escherichia coli O157:H7 and Salmonella enterica are evaluated. The effect of wintergreen essential oil derived from Gaultheria procumbens was analyzed against E. coli and S. enterica in concentrations of 5 μL/1.5 mL, 2.5 μL/1.5 mL, and 1 μL/1.5 mL (wintergreen/sterilized deionized water). Experiments of UV lamp exposure, with and without immobilized TiO2 photocatalyst, were performed to determine pathogen inactivation efficiency. Sunlight alone and with immobilized TiO2 photocatalyst were studied for pathogen inactivation potential as well. Lastly, physical filtration via portable backpacking pump filter and purification by emergency chlorine dioxide and iodine tablets were tested for removal and purification efficiencies against E. coli and S. enterica. Bacterial viability was quantified by serial dilution and viable plate counting. Preliminary experiments were also done to develop methods and procedures, including the effect of refrigerated storage on E. coli viability in Tryptic Soy Broth and the development of E. coli and S. enterica growth curves by optical density at 600 nm (OD 600 ) with spectrophotometry measurements. Results of these experiments are discussed and compared. Statistical analysis via one-way ANOVA with a post-hoc Tukey HSD was used to analyze difference in treatments for significance. Results showed pathogen removal for all technologies studied, to varying degrees. Some examples include: 5-log reduction was achieved in E. coli with sunlight-only at 6 hours; 3-log reduction was achieved in S. enterica with sunlight-only at 7.5 hours, E. coli UV lamp-only at 8 hours, E. coli and S. enterica with sunlight/TiO2 at 6 and 7.5 hours, respectively, and E. coli with a 5 μL concentration of Wintergreen essential oil at 50 minutes; 3-log reduction in E. coli was observed when the 5 μL concentration of wintergreen essential oil was applied for 50 minutes, 2-log reduction in E. coli was observed after 50 minutes of 2.5 μL concentration treatment, while the 1 μL concentration of wintergreen essential oil achieved only a 40% reduction in E. coli at 50 minutes. The results from this research may influence further pursuit in one or combinations of these water treatment technologies.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-08-01","The student, Matthew Miller, accepted the attached license on 2021-07-23 at 15:18.","The student, Matthew Miller, submitted this Thesis for approval on 2021-07-23 at 15:24.","This Thesis was approved for publication on 2021-07-23 at 15:56.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17090 on 2022-01-12 at 13:05:36","Made available in DSpace on 2022-01-12T22:56:16Z (GMT). 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Human activities, including large industries in agriculture and manufacturing, produce massive amounts of waste that invariably end up in water supplies. There will always be a need for water treatment technologies that evolve and progress as waste volume and complexity increases. Further exploration and development of current treatment methods will be necessary to match this demand. In this study, wintergreen essential oil, physical filtration, iodine and chlorine dioxide tablets, sunlight, artificial UV light, and TiO2 photocatalyst treatment methods and their effects on waterborne pathogens Escherichia coli O157:H7 and Salmonella enterica are evaluated. The effect of wintergreen essential oil derived from Gaultheria procumbens was analyzed against E. coli and S. enterica in concentrations of 5 μL/1.5 mL, 2.5 μL/1.5 mL, and 1 μL/1.5 mL (wintergreen/sterilized deionized water). Experiments of UV lamp exposure, with and without immobilized TiO2 photocatalyst, were performed to determine pathogen inactivation efficiency. Sunlight alone and with immobilized TiO2 photocatalyst were studied for pathogen inactivation potential as well. Lastly, physical filtration via portable backpacking pump filter and purification by emergency chlorine dioxide and iodine tablets were tested for removal and purification efficiencies against E. coli and S. enterica. Bacterial viability was quantified by serial dilution and viable plate counting. Preliminary experiments were also done to develop methods and procedures, including the effect of refrigerated storage on E. coli viability in Tryptic Soy Broth and the development of E. coli and S. enterica growth curves by optical density at 600 nm (OD 600 ) with spectrophotometry measurements. Results of these experiments are discussed and compared. Statistical analysis via one-way ANOVA with a post-hoc Tukey HSD was used to analyze difference in treatments for significance. Results showed pathogen removal for all technologies studied, to varying degrees. Some examples include: 5-log reduction was achieved in E. coli with sunlight-only at 6 hours; 3-log reduction was achieved in S. enterica with sunlight-only at 7.5 hours, E. coli UV lamp-only at 8 hours, E. coli and S. enterica with sunlight/TiO2 at 6 and 7.5 hours, respectively, and E. coli with a 5 μL concentration of Wintergreen essential oil at 50 minutes; 3-log reduction in E. coli was observed when the 5 μL concentration of wintergreen essential oil was applied for 50 minutes, 2-log reduction in E. coli was observed after 50 minutes of 2.5 μL concentration treatment, while the 1 μL concentration of wintergreen essential oil achieved only a 40% reduction in E. coli at 50 minutes. The results from this research may influence further pursuit in one or combinations of these water treatment technologies.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-08-01","The student, Matthew Miller, accepted the attached license on 2021-07-23 at 15:18.","The student, Matthew Miller, submitted this Thesis for approval on 2021-07-23 at 15:24.","This Thesis was approved for publication on 2021-07-23 at 15:56.","DSpace SAF Submission Ingestion Package generated from Vireo submission #17090 on 2022-01-12 at 13:05:36","Made available in DSpace on 2022-01-12T22:56:16Z (GMT). No. of bitstreams: 2 MILLER-THESIS-2021.pdf: 2825533 bytes, checksum: de1a556548de73f575afd0fcba41bf5d (MD5) LICENSE.txt: 4211 bytes, checksum: 240e63abc4f22cdd2f5a811da4dc3c53 (MD5) Previous issue date: 2021-07-23","Embargo set by: Seth Robbins for item 121282 Lift date: 2024-01-12T22:56:20Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/113353"],"dc:language":["en"],"dc:rights":["Copyright 2021 Matthew Miller"],"dc:subject":["Escherichia coli O157:H7","Salmonella enterica"],"dc:title":["Evaluation of Escherichia coli O157:H7 and Salmonella enterica inactivation in water by several treatment technologies"],"dc:type":["Thesis","text"],"thesis:degree_discipline":["Agricultural & Biological Engr"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:53Z"}