Measurement of the cellular oxidative potential and cytotoxicity of ambient pm2.5 – an assessment of toxic pm2.5 chemical components and their sources in the midwest US
type: CreativeWork
description: Ambient fine particulate matter (particles smaller than 2.5 µm in size or PM2.5 ) is the 4th leading risk factor for global mortality. PM2.5 has a unique toxic mechanism that utilizes both cellular antioxidants and reductants to catalyze the generation of reactive oxygen species (ROS). Excessive accumulation of ROS in the biological system might lead to cellular damage and even apoptosis. The capability to create this imbalance of ROS accumulation in the biological system is called PM2.5 oxidative potential (OP). There are both cellular and acellular methods to measure the OP of ambient PM2.5 . However, despite a commonly wide application of these assays in PM2.5 studies, there are two major questions still unanswered: 1. What is the biological relevance of chemical methods to measure the OP, and 2. What are the chemical components driving the response of both cellular and acellular OP. This dissertation aims to address some of these questions for the associations among PM 2.5 OP, cytotoxicity, and PM2.5 components. The biological relevance of PM2.5 chemical OP assays hasn’t been well established. The first research objective in this dissertation is to link chemical OP assays with cytotoxicity. PM2.5 samples were collected from an urban site in Champaign, IL. The cytotoxicity was assessed by a Chinese hamster ovary (CHO) cells cytotoxicity assay. The PM2.5 OP was measured by three acellular OP assays. The carbonaceous components and concentration of metals in the water-soluble PM2.5 were also determined. Correlation analysis among the PM2.5 OP, cytotoxicity and chemical composition was conducted. All three endpoints in the OP assays correlated significantly with cytotoxicity. The chemical OP assays that measure the •OH generation had the best association with the cytotoxicity. This should be attributed to their capability of capturing the synergistic interactions among organic compounds (i.e., water-soluble organic carbon (WSOC)) and metals (Fe and Cu). To further c…
abstract: Ambient fine particulate matter (particles smaller than 2.5 µm in size or PM2.5 ) is the 4th leading risk factor for global mortality. PM2.5 has a unique toxic mechanism that utilizes both cellular antioxidants and reductants to catalyze the generation of reactive oxygen species (ROS). Excessive accumulation of ROS in the biological system might lead to cellular damage and even apoptosis. The capability to create this imbalance of ROS accumulation in the biological system is called PM2.5 oxidative potential (OP). There are both cellular and acellular methods to measure the OP of ambient PM2.5 . However, despite a commonly wide application of these assays in PM2.5 studies, there are two major questions still unanswered: 1. What is the biological relevance of chemical methods to measure the OP, and 2. What are the chemical components driving the response of both cellular and acellular OP. This dissertation aims to address some of these questions for the associations among PM 2.5 OP, cytotoxicity, and PM2.5 components. The biological relevance of PM2.5 chemical OP assays hasn’t been well established. The first research objective in this dissertation is to link chemical OP assays with cytotoxicity. PM2.5 samples were collected from an urban site in Champaign, IL. The cytotoxicity was assessed by a Chinese hamster ovary (CHO) cells cytotoxicity assay. The PM2.5 OP was measured by three acellular OP assays. The carbonaceous components and concentration of metals in the water-soluble PM2.5 were also determined. Correlation analysis among the PM2.5 OP, cytotoxicity and chemical composition was conducted. All three endpoints in the OP assays correlated significantly with cytotoxicity. The chemical OP assays that measure the •OH generation had the best association with the cytotoxicity. This should be attributed to their capability of capturing the synergistic interactions among organic compounds (i.e., water-soluble organic carbon (WSOC)) and metals (Fe and Cu). To further c…
author: Wang, Yixiang
language: en
keywords: Cytotoxicity, Chinese hamster ovary (CHO) cells, Oxidative Potential, Reactive oxygen species (ROS) generation, Dithiothreitol, Interactions, Macrophage, Midwest
date published: 2022-01-12T22:55:02Z
date modified: 2026-07-22T22:24:53Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/113294