{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/110865"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/110865","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Dietary protein ameliorates goblet cell loss and modifies inflammatory responses in the colon of hnRNPI knockout mouse","abstract":"Heterogeneous nuclear ribonucleoprotein I (hnRNPI) is an essential RNA binding protein that plays a critical role in regulating neonatal immune adaptation through downregulation of interleukin-1 receptor-associated kinase (IRAK1) in toll-like receptor (TLR)-mediated NF-κB signaling. Transgenic mice with hnRNPI specifically knockout in intestinal epithelial cells (IECs) develop spontaneous colitis at an early age. Moderate dietary protein has been demonstrated to exert beneficial effects for individuals with colitis, however, mechanisms of how dietary protein impacts inflammation development and immune responses are still unclear. This study aims to examine the potential roles of dietary protein in regulating goblet cells and inflammatory responses in a mouse model with IECs-specific hnRNPI knockout‐induced colitis. Physiological outcomes were assessed by body weight, growth rate, colon parameters and histology analysis. Goblet cells were quantified by staining. Relative mRNA levels of inflammation- and goblet cell-associated genes were examined using quantitative real-time PCR. CD4+ T cell subpopulations in the distal colon were measured by flow cytometry analysis. The results demonstrated that medium dietary protein prevents goblet cell loss and alters inflammatory responses with an increase in IL17- IFNγ- CD4+ T cells. Notably, we found that primary and dominant immune responses in hnRNPI knockout mice were significant increases in regulatory T cells and IL17- IFNγ- CD4+ T cells in the colon. Collectively, these results demonstrated that medium dietary protein ameliorates goblet cell loss and modifies inflammatory responses with an increase in IL17- IFNγ- CD4+ T cells in the colon of IECs- specific hnRNPI knockout mouse.","abstract_html":"Heterogeneous nuclear ribonucleoprotein I (hnRNPI) is an essential RNA binding protein that plays a critical role in regulating neonatal immune adaptation through downregulation of interleukin-1 receptor-associated kinase (IRAK1) in toll-like receptor (TLR)-mediated NF-κB signaling. Transgenic mice with hnRNPI specifically knockout in intestinal epithelial cells (IECs) develop spontaneous colitis at an early age. Moderate dietary protein has been demonstrated to exert beneficial effects for individuals with colitis, however, mechanisms of how dietary protein impacts inflammation development and immune responses are still unclear. This study aims to examine the potential roles of dietary protein in regulating goblet cells and inflammatory responses in a mouse model with IECs-specific hnRNPI knockout‐induced colitis. Physiological outcomes were assessed by body weight, growth rate, colon parameters and histology analysis. Goblet cells were quantified by staining. Relative mRNA levels of inflammation- and goblet cell-associated genes were examined using quantitative real-time PCR. CD4+ T cell subpopulations in the distal colon were measured by flow cytometry analysis. The results demonstrated that medium dietary protein prevents goblet cell loss and alters inflammatory responses with an increase in IL17- IFNγ- CD4+ T cells. Notably, we found that primary and dominant immune responses in hnRNPI knockout mice were significant increases in regulatory T cells and IL17- IFNγ- CD4+ T cells in the colon. Collectively, these results demonstrated that medium dietary protein ameliorates goblet cell loss and modifies inflammatory responses with an increase in IL17- IFNγ- CD4+ T cells in the colon of IECs- specific hnRNPI knockout mouse.","abstract_has_math":false,"creators":["Li, Jiaxuan"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Chen, Hong","Pan, Yuan-Xiang","McKim, Daniel B."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-09-17T04:06:54Z","date_published":"2021-09-17T04:06:54Z","updated_at":"2026-07-22T22:24:52Z","subjects":["hnRNPI","PTB","protein diet","goblet cell","inflammation","colitis","immune response","CD4+ T cell"],"languages":["en"],"rights":["Copyright 2021 Jiaxuan Li"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/110865","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Chen, Hong","Pan, Yuan-Xiang","McKim, Daniel B."]},{"key":"dc:creator","label":"Author","values":["Li, Jiaxuan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-09-17T04:06:54Z","2023-09-17T04:07:01Z","2021-04-28","2021-05"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["hnRNPI","PTB","protein diet","goblet cell","inflammation","colitis","immune response","CD4+ T cell"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Jiaxuan Li"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/110865"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Heterogeneous nuclear ribonucleoprotein I (hnRNPI) is an essential RNA binding protein that plays a critical role in regulating neonatal immune adaptation through downregulation of interleukin-1 receptor-associated kinase (IRAK1) in toll-like receptor (TLR)-mediated NF-κB signaling. Transgenic mice with hnRNPI specifically knockout in intestinal epithelial cells (IECs) develop spontaneous colitis at an early age. Moderate dietary protein has been demonstrated to exert beneficial effects for individuals with colitis, however, mechanisms of how dietary protein impacts inflammation development and immune responses are still unclear. This study aims to examine the potential roles of dietary protein in regulating goblet cells and inflammatory responses in a mouse model with IECs-specific hnRNPI knockout‐induced colitis. Physiological outcomes were assessed by body weight, growth rate, colon parameters and histology analysis. Goblet cells were quantified by staining. Relative mRNA levels of inflammation- and goblet cell-associated genes were examined using quantitative real-time PCR. CD4+ T cell subpopulations in the distal colon were measured by flow cytometry analysis. The results demonstrated that medium dietary protein prevents goblet cell loss and alters inflammatory responses with an increase in IL17- IFNγ- CD4+ T cells. Notably, we found that primary and dominant immune responses in hnRNPI knockout mice were significant increases in regulatory T cells and IL17- IFNγ- CD4+ T cells in the colon. Collectively, these results demonstrated that medium dietary protein ameliorates goblet cell loss and modifies inflammatory responses with an increase in IL17- IFNγ- CD4+ T cells in the colon of IECs- specific hnRNPI knockout mouse.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Jiaxuan Li, accepted the attached license on 2021-04-27 at 09:34.","The student, Jiaxuan Li, submitted this Thesis for approval on 2021-04-27 at 09:57.","This Thesis was approved for publication on 2021-04-28 at 14:45.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16580 on 2021-09-16 at 20:14:31","Made available in DSpace on 2021-09-17T04:06:54Z (GMT). No. of bitstreams: 2 LI-THESIS-2021.pdf: 1473520 bytes, checksum: 210acb9fda2b19111c384858ded0fa44 (MD5) LICENSE.txt: 4207 bytes, checksum: 2996f5be725b4213f277ac3dcda6ce3e (MD5) Previous issue date: 2021-04-28","Embargo set by: Seth Robbins for item 118711 Lift date: 2023-09-17T04:07:01Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Dietary protein ameliorates goblet cell loss and modifies inflammatory responses in the colon of hnRNPI knockout mouse"]}]}],"canonical_facts":{"dc:contributor":["Chen, Hong","Pan, Yuan-Xiang","McKim, Daniel B."],"dc:creator":["Li, Jiaxuan"],"dc:date":["2021-09-17T04:06:54Z","2023-09-17T04:07:01Z","2021-04-28","2021-05"],"dc:description":["Heterogeneous nuclear ribonucleoprotein I (hnRNPI) is an essential RNA binding protein that plays a critical role in regulating neonatal immune adaptation through downregulation of interleukin-1 receptor-associated kinase (IRAK1) in toll-like receptor (TLR)-mediated NF-κB signaling. Transgenic mice with hnRNPI specifically knockout in intestinal epithelial cells (IECs) develop spontaneous colitis at an early age. Moderate dietary protein has been demonstrated to exert beneficial effects for individuals with colitis, however, mechanisms of how dietary protein impacts inflammation development and immune responses are still unclear. This study aims to examine the potential roles of dietary protein in regulating goblet cells and inflammatory responses in a mouse model with IECs-specific hnRNPI knockout‐induced colitis. Physiological outcomes were assessed by body weight, growth rate, colon parameters and histology analysis. Goblet cells were quantified by staining. Relative mRNA levels of inflammation- and goblet cell-associated genes were examined using quantitative real-time PCR. CD4+ T cell subpopulations in the distal colon were measured by flow cytometry analysis. The results demonstrated that medium dietary protein prevents goblet cell loss and alters inflammatory responses with an increase in IL17- IFNγ- CD4+ T cells. Notably, we found that primary and dominant immune responses in hnRNPI knockout mice were significant increases in regulatory T cells and IL17- IFNγ- CD4+ T cells in the colon. Collectively, these results demonstrated that medium dietary protein ameliorates goblet cell loss and modifies inflammatory responses with an increase in IL17- IFNγ- CD4+ T cells in the colon of IECs- specific hnRNPI knockout mouse.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Jiaxuan Li, accepted the attached license on 2021-04-27 at 09:34.","The student, Jiaxuan Li, submitted this Thesis for approval on 2021-04-27 at 09:57.","This Thesis was approved for publication on 2021-04-28 at 14:45.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16580 on 2021-09-16 at 20:14:31","Made available in DSpace on 2021-09-17T04:06:54Z (GMT). No. of bitstreams: 2 LI-THESIS-2021.pdf: 1473520 bytes, checksum: 210acb9fda2b19111c384858ded0fa44 (MD5) LICENSE.txt: 4207 bytes, checksum: 2996f5be725b4213f277ac3dcda6ce3e (MD5) Previous issue date: 2021-04-28","Embargo set by: Seth Robbins for item 118711 Lift date: 2023-09-17T04:07:01Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/110865"],"dc:language":["en"],"dc:rights":["Copyright 2021 Jiaxuan Li"],"dc:subject":["hnRNPI","PTB","protein diet","goblet cell","inflammation","colitis","immune response","CD4+ T cell"],"dc:title":["Dietary protein ameliorates goblet cell loss and modifies inflammatory responses in the colon of hnRNPI knockout mouse"],"dc:type":["Thesis","text"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:52Z"}