{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/110862"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/110862","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Investigating the role of phosphate homeostasis in Staphylococcus aureus virulence","abstract":"Due to its use in DNA, energy storage, intracellular signaling, and other important biological processes, phosphate is an essential nutrient for all organisms. Bacteria possess a variety of complex phosphate sensing and uptake systems to ensure that they meet their need for phosphate. The ubiquity and necessity of these systems make them a possible target for novel therapeutics to treat antibiotic-resistant bacteria such as Staphylococcus aureus. Previous studies in S. aureus have shown that disruption of phosphate homeostasis by overexpression of the PstSCAB phosphate transporter or loss of function of the PhoPR phosphate sensing system reduces virulence. However, how phosphate homeostasis is controlled in S. aureus is yet to be discerned. S. aureus codes for three accessory regulators that may modulate phosphate homeostasis. The current work revealed that one of these, PitR, is the main regulator of PhoPR activity. Preliminary evidence suggests that the other two homologs, PhoU and the PhoU-like domain of NptA may also regulate PhoPR activity and phosphate uptake.","abstract_html":"Due to its use in DNA, energy storage, intracellular signaling, and other important biological processes, phosphate is an essential nutrient for all organisms. Bacteria possess a variety of complex phosphate sensing and uptake systems to ensure that they meet their need for phosphate. The ubiquity and necessity of these systems make them a possible target for novel therapeutics to treat antibiotic-resistant bacteria such as Staphylococcus aureus. Previous studies in S. aureus have shown that disruption of phosphate homeostasis by overexpression of the PstSCAB phosphate transporter or loss of function of the PhoPR phosphate sensing system reduces virulence. However, how phosphate homeostasis is controlled in S. aureus is yet to be discerned. S. aureus codes for three accessory regulators that may modulate phosphate homeostasis. The current work revealed that one of these, PitR, is the main regulator of PhoPR activity. Preliminary evidence suggests that the other two homologs, PhoU and the PhoU-like domain of NptA may also regulate PhoPR activity and phosphate uptake.","abstract_has_math":false,"creators":["Joya Sandoval, Eliot Scottie"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Kehl-Fie, Thomas E","Metcalf, William W","Wilson, Brenda A"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-09-17T04:06:54Z","date_published":"2021-09-17T04:06:54Z","updated_at":"2026-07-22T22:24:52Z","subjects":["Staphylococcus aureus","phosphate","phosphate homeostasis","PhoPR","PhoU","PstSCAB","NptA","PitR","PitA","antibiotic resistance"],"languages":["en"],"rights":["Copyright 2021 Eliot Joya Sandoval"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/110862","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kehl-Fie, Thomas E","Metcalf, William W","Wilson, Brenda A"]},{"key":"dc:creator","label":"Author","values":["Joya Sandoval, Eliot Scottie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-09-17T04:06:54Z","2023-09-17T04:07:01Z","2021-04-28","2021-05"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Staphylococcus aureus","phosphate","phosphate homeostasis","PhoPR","PhoU","PstSCAB","NptA","PitR","PitA","antibiotic resistance"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Eliot Joya Sandoval"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/110862"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Due to its use in DNA, energy storage, intracellular signaling, and other important biological processes, phosphate is an essential nutrient for all organisms. Bacteria possess a variety of complex phosphate sensing and uptake systems to ensure that they meet their need for phosphate. The ubiquity and necessity of these systems make them a possible target for novel therapeutics to treat antibiotic-resistant bacteria such as Staphylococcus aureus. Previous studies in S. aureus have shown that disruption of phosphate homeostasis by overexpression of the PstSCAB phosphate transporter or loss of function of the PhoPR phosphate sensing system reduces virulence. However, how phosphate homeostasis is controlled in S. aureus is yet to be discerned. S. aureus codes for three accessory regulators that may modulate phosphate homeostasis. The current work revealed that one of these, PitR, is the main regulator of PhoPR activity. Preliminary evidence suggests that the other two homologs, PhoU and the PhoU-like domain of NptA may also regulate PhoPR activity and phosphate uptake.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Eliot Joya Sandoval, accepted the attached license on 2021-04-26 at 17:13.","The student, Eliot Joya Sandoval, submitted this Thesis for approval on 2021-04-27 at 13:28.","This Thesis was approved for publication on 2021-04-28 at 09:25.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16568 on 2021-09-16 at 20:14:26","Made available in DSpace on 2021-09-17T04:06:54Z (GMT). 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Bacteria possess a variety of complex phosphate sensing and uptake systems to ensure that they meet their need for phosphate. The ubiquity and necessity of these systems make them a possible target for novel therapeutics to treat antibiotic-resistant bacteria such as Staphylococcus aureus. Previous studies in S. aureus have shown that disruption of phosphate homeostasis by overexpression of the PstSCAB phosphate transporter or loss of function of the PhoPR phosphate sensing system reduces virulence. However, how phosphate homeostasis is controlled in S. aureus is yet to be discerned. S. aureus codes for three accessory regulators that may modulate phosphate homeostasis. The current work revealed that one of these, PitR, is the main regulator of PhoPR activity. Preliminary evidence suggests that the other two homologs, PhoU and the PhoU-like domain of NptA may also regulate PhoPR activity and phosphate uptake.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Eliot Joya Sandoval, accepted the attached license on 2021-04-26 at 17:13.","The student, Eliot Joya Sandoval, submitted this Thesis for approval on 2021-04-27 at 13:28.","This Thesis was approved for publication on 2021-04-28 at 09:25.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16568 on 2021-09-16 at 20:14:26","Made available in DSpace on 2021-09-17T04:06:54Z (GMT). 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