{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/110807"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/110807","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Developing disease-state liver models to determine the effects on brain function in vitro","abstract":"The liver is responsible for filtering and detoxifying harmful chemicals from the body. Because of its role as the barrier between the gut and the circulatory system, and due to the brain’s high perfusion rate, the liver’s function as a regulator for blood composition is highly important for the brain. Although the blood brain barrier blocks certain large molecules from entering into the organ, small molecules, such as ammonia, can freely pass through the fenestrations in the barrier or are naturally transported by the endothelial cells. When damage occurs to the liver and there are alterations in behavior or cognition, the condition is termed hepatic encephalopathy. Therefore, the health of the liver is important for regulating the function of the brain. However, the methods for creating in vitro hepatoid models with varying levels of dysfunction are not well developed. With this in mind, my doctoral research examines the role of using tunable collagen-polyethylene glycol hydrogels to modulate the response of hepatoids and hepatocarcinoma to exogenous factors. Chapter 2 investigates how the morphology and functionality of the zebrafish liver is degraded by endocrine disrupting chemicals, such as estrogen and bisphenol-A. Chapter 3 explores methods for developing a hepatocarcinoma model which can depict varying levels of matrix invasion and functionality. Finally, Chapter 4 investigates how collagen-hyaluronic acid hydrogels affect cortical neuron cluster morphology and the resulting change in neurotransmitter production when exposed to the hyperammonemia conditions present during hepatic encephalopathy.","abstract_html":"The liver is responsible for filtering and detoxifying harmful chemicals from the body. Because of its role as the barrier between the gut and the circulatory system, and due to the brain’s high perfusion rate, the liver’s function as a regulator for blood composition is highly important for the brain. Although the blood brain barrier blocks certain large molecules from entering into the organ, small molecules, such as ammonia, can freely pass through the fenestrations in the barrier or are naturally transported by the endothelial cells. When damage occurs to the liver and there are alterations in behavior or cognition, the condition is termed hepatic encephalopathy. Therefore, the health of the liver is important for regulating the function of the brain. However, the methods for creating in vitro hepatoid models with varying levels of dysfunction are not well developed. With this in mind, my doctoral research examines the role of using tunable collagen-polyethylene glycol hydrogels to modulate the response of hepatoids and hepatocarcinoma to exogenous factors. Chapter 2 investigates how the morphology and functionality of the zebrafish liver is degraded by endocrine disrupting chemicals, such as estrogen and bisphenol-A. Chapter 3 explores methods for developing a hepatocarcinoma model which can depict varying levels of matrix invasion and functionality. Finally, Chapter 4 investigates how collagen-hyaluronic acid hydrogels affect cortical neuron cluster morphology and the resulting change in neurotransmitter production when exposed to the hyperammonemia conditions present during hepatic encephalopathy.","abstract_has_math":false,"creators":["Sullivan, Kathryn Michele"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Kong, Hyunjoon","Underhill, Gregory","Gillette, Martha","Dar, Roy"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-09-17T04:04:23Z","date_published":"2021-09-17T04:04:23Z","updated_at":"2026-07-22T22:24:52Z","subjects":["liver organoid","liver cancer","hydrogel","endocrine disrupting chemical","neurons","hyperammonemia"],"languages":["en"],"rights":["Copyright 2021 Kathryn Sullivan"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/110807","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kong, Hyunjoon","Underhill, Gregory","Gillette, Martha","Dar, Roy"]},{"key":"dc:creator","label":"Author","values":["Sullivan, Kathryn Michele"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-09-17T04:04:23Z","2023-09-17T04:07:01Z","2021-04-21","2021-05"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["liver organoid","liver cancer","hydrogel","endocrine disrupting chemical","neurons","hyperammonemia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Kathryn Sullivan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/110807"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The liver is responsible for filtering and detoxifying harmful chemicals from the body. Because of its role as the barrier between the gut and the circulatory system, and due to the brain’s high perfusion rate, the liver’s function as a regulator for blood composition is highly important for the brain. Although the blood brain barrier blocks certain large molecules from entering into the organ, small molecules, such as ammonia, can freely pass through the fenestrations in the barrier or are naturally transported by the endothelial cells. When damage occurs to the liver and there are alterations in behavior or cognition, the condition is termed hepatic encephalopathy. Therefore, the health of the liver is important for regulating the function of the brain. However, the methods for creating in vitro hepatoid models with varying levels of dysfunction are not well developed. With this in mind, my doctoral research examines the role of using tunable collagen-polyethylene glycol hydrogels to modulate the response of hepatoids and hepatocarcinoma to exogenous factors. Chapter 2 investigates how the morphology and functionality of the zebrafish liver is degraded by endocrine disrupting chemicals, such as estrogen and bisphenol-A. Chapter 3 explores methods for developing a hepatocarcinoma model which can depict varying levels of matrix invasion and functionality. Finally, Chapter 4 investigates how collagen-hyaluronic acid hydrogels affect cortical neuron cluster morphology and the resulting change in neurotransmitter production when exposed to the hyperammonemia conditions present during hepatic encephalopathy.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Kathryn Sullivan, accepted the attached license on 2021-04-12 at 16:31.","The student, Kathryn Sullivan, submitted this Dissertation for approval on 2021-04-12 at 16:41.","This Dissertation was approved for publication on 2021-04-21 at 14:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16294 on 2021-09-16 at 20:08:37","Made available in DSpace on 2021-09-17T04:04:23Z (GMT). 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Because of its role as the barrier between the gut and the circulatory system, and due to the brain’s high perfusion rate, the liver’s function as a regulator for blood composition is highly important for the brain. Although the blood brain barrier blocks certain large molecules from entering into the organ, small molecules, such as ammonia, can freely pass through the fenestrations in the barrier or are naturally transported by the endothelial cells. When damage occurs to the liver and there are alterations in behavior or cognition, the condition is termed hepatic encephalopathy. Therefore, the health of the liver is important for regulating the function of the brain. However, the methods for creating in vitro hepatoid models with varying levels of dysfunction are not well developed. With this in mind, my doctoral research examines the role of using tunable collagen-polyethylene glycol hydrogels to modulate the response of hepatoids and hepatocarcinoma to exogenous factors. Chapter 2 investigates how the morphology and functionality of the zebrafish liver is degraded by endocrine disrupting chemicals, such as estrogen and bisphenol-A. Chapter 3 explores methods for developing a hepatocarcinoma model which can depict varying levels of matrix invasion and functionality. Finally, Chapter 4 investigates how collagen-hyaluronic acid hydrogels affect cortical neuron cluster morphology and the resulting change in neurotransmitter production when exposed to the hyperammonemia conditions present during hepatic encephalopathy.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Kathryn Sullivan, accepted the attached license on 2021-04-12 at 16:31.","The student, Kathryn Sullivan, submitted this Dissertation for approval on 2021-04-12 at 16:41.","This Dissertation was approved for publication on 2021-04-21 at 14:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16294 on 2021-09-16 at 20:08:37","Made available in DSpace on 2021-09-17T04:04:23Z (GMT). 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