{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/109492"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/109492","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Determining the beneficial properties of bovine amniotic membrane and its efficacy as a therapy for corneal healing","abstract":"Corneal ulceration, or ulcerative keratitis, is one of the most common ocular diseases observed in dogs and horses, particularly after trauma. Superficial corneal ulcers can heal without complications in a noninfected environment, but severe ulcerative keratitis can progress rapidly and lead to impaired vision and loss of the eye. Corneal healing is modulated by complex signaling pathways which involve proteinases, proteinase inhibitors, growth factors, and pro- and anti-inflammatory cytokines. For adequate corneal healing, it is necessary to have a well-balanced interaction between proteinases and their inhibitors, as well as anti-angiogenic and angiogenic factors. Therefore, keeping this balance should be the target approach in treating ulcerative keratitis. Some medications can alter the ocular microenvironment and help keep this balance, improving corneal healing. In this dissertation work, bovine amniotic membrane homogenate (BAMH) has been investigated to evaluate its efficacy as therapy during corneal healing. The first step was to confirm if BAMH contains tissue inhibitor of metalloproteinases (TIMPs), the most effective metalloproteinase inhibitors in the cornea, and if BAMH is able to inhibit metalloproteinases (MMPs) -2 and -9, which are detrimental when in excess. After confirming these properties in vitro, BAMH effectiveness during healing process was evaluated in an ex vivo wounded cornea model, topically applying the BAMH and comparing its effect with EDTA and a control. The BAMH was able to reduce corneal healing time significantly when compared to the controls, suggesting that it is a promising treatment for stromal and epithelial ulcers. Subsequently, the next step was to develop a protein profile of the bovine amniotic membrane (BAM) to understand how BAMH could potentially influence corneal ulcer therapy. To do so, BAM proteins were identified using high-throughput proteomic analysis. The results revealed that many of the expressed proteins in the BAM were also present in the cornea and involved in several biological and molecular functions. In conclusion, BAMH appears to be a promising treatment for corneal ulcers, presenting TIMP -1, TIMP -2 and TIMP -3, inhibiting MMP-2 and MMP-9 in vitro, accelerating the corneal healing time, and presenting numerous beneficial proteins important for corneal health and function.","abstract_html":"Corneal ulceration, or ulcerative keratitis, is one of the most common ocular diseases observed in dogs and horses, particularly after trauma. Superficial corneal ulcers can heal without complications in a noninfected environment, but severe ulcerative keratitis can progress rapidly and lead to impaired vision and loss of the eye. Corneal healing is modulated by complex signaling pathways which involve proteinases, proteinase inhibitors, growth factors, and pro- and anti-inflammatory cytokines. For adequate corneal healing, it is necessary to have a well-balanced interaction between proteinases and their inhibitors, as well as anti-angiogenic and angiogenic factors. Therefore, keeping this balance should be the target approach in treating ulcerative keratitis. Some medications can alter the ocular microenvironment and help keep this balance, improving corneal healing. In this dissertation work, bovine amniotic membrane homogenate (BAMH) has been investigated to evaluate its efficacy as therapy during corneal healing. The first step was to confirm if BAMH contains tissue inhibitor of metalloproteinases (TIMPs), the most effective metalloproteinase inhibitors in the cornea, and if BAMH is able to inhibit metalloproteinases (MMPs) -2 and -9, which are detrimental when in excess. After confirming these properties in vitro, BAMH effectiveness during healing process was evaluated in an ex vivo wounded cornea model, topically applying the BAMH and comparing its effect with EDTA and a control. The BAMH was able to reduce corneal healing time significantly when compared to the controls, suggesting that it is a promising treatment for stromal and epithelial ulcers. Subsequently, the next step was to develop a protein profile of the bovine amniotic membrane (BAM) to understand how BAMH could potentially influence corneal ulcer therapy. To do so, BAM proteins were identified using high-throughput proteomic analysis. The results revealed that many of the expressed proteins in the BAM were also present in the cornea and involved in several biological and molecular functions. In conclusion, BAMH appears to be a promising treatment for corneal ulcers, presenting TIMP -1, TIMP -2 and TIMP -3, inhibiting MMP-2 and MMP-9 in vitro, accelerating the corneal healing time, and presenting numerous beneficial proteins important for corneal health and function.","abstract_has_math":false,"creators":["Capistrano Da Silva, Erotides"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"VMS - Comparative Biosciences","degree_department":null,"school":null,"contributors":["Flaws, Jodi A","da Costa Martins, Bianca","Mahoney, Megan M","Fleming, Kathryn M"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-03-05T21:40:43Z","date_published":"2021-03-05T21:40:43Z","updated_at":"2026-07-22T22:24:50Z","subjects":["Bovine amniotic membrane, homogenate, metalloproteinases (MMPs), TIMPs, corneal healing, proteomic composition, ex vivo corneas, in vitro MMPs -2 and -9 reductive activities"],"languages":["en"],"rights":["Copyright 2020 Erotides Capistrano Da Silva"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/109492","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Flaws, Jodi A","da Costa Martins, Bianca","Mahoney, Megan M","Fleming, Kathryn M"]},{"key":"dc:creator","label":"Author","values":["Capistrano Da Silva, Erotides"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-03-05T21:40:43Z","2023-03-05T21:43:00Z","2020-11-25","2020-12"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["VMS - Comparative Biosciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bovine amniotic membrane, homogenate, metalloproteinases (MMPs), TIMPs, corneal healing, proteomic composition, ex vivo corneas, in vitro MMPs -2 and -9 reductive activities"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2020 Erotides Capistrano Da Silva"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/109492"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Corneal ulceration, or ulcerative keratitis, is one of the most common ocular diseases observed in dogs and horses, particularly after trauma. Superficial corneal ulcers can heal without complications in a noninfected environment, but severe ulcerative keratitis can progress rapidly and lead to impaired vision and loss of the eye. Corneal healing is modulated by complex signaling pathways which involve proteinases, proteinase inhibitors, growth factors, and pro- and anti-inflammatory cytokines. For adequate corneal healing, it is necessary to have a well-balanced interaction between proteinases and their inhibitors, as well as anti-angiogenic and angiogenic factors. Therefore, keeping this balance should be the target approach in treating ulcerative keratitis. Some medications can alter the ocular microenvironment and help keep this balance, improving corneal healing. In this dissertation work, bovine amniotic membrane homogenate (BAMH) has been investigated to evaluate its efficacy as therapy during corneal healing. The first step was to confirm if BAMH contains tissue inhibitor of metalloproteinases (TIMPs), the most effective metalloproteinase inhibitors in the cornea, and if BAMH is able to inhibit metalloproteinases (MMPs) -2 and -9, which are detrimental when in excess. After confirming these properties in vitro, BAMH effectiveness during healing process was evaluated in an ex vivo wounded cornea model, topically applying the BAMH and comparing its effect with EDTA and a control. The BAMH was able to reduce corneal healing time significantly when compared to the controls, suggesting that it is a promising treatment for stromal and epithelial ulcers. Subsequently, the next step was to develop a protein profile of the bovine amniotic membrane (BAM) to understand how BAMH could potentially influence corneal ulcer therapy. To do so, BAM proteins were identified using high-throughput proteomic analysis. The results revealed that many of the expressed proteins in the BAM were also present in the cornea and involved in several biological and molecular functions. In conclusion, BAMH appears to be a promising treatment for corneal ulcers, presenting TIMP -1, TIMP -2 and TIMP -3, inhibiting MMP-2 and MMP-9 in vitro, accelerating the corneal healing time, and presenting numerous beneficial proteins important for corneal health and function.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2022-12-01","The student, Erotides Capistrano Da Silva, accepted the attached license on 2020-11-18 at 14:11.","The student, Erotides Capistrano Da Silva, submitted this Dissertation for approval on 2020-11-18 at 14:28.","This Dissertation was approved for publication on 2020-11-25 at 11:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #15903 on 2021-03-04 at 16:19:33","Made available in DSpace on 2021-03-05T21:40:43Z (GMT). No. of bitstreams: 2 CAPISTRANODASILVA-DISSERTATION-2020.pdf: 2520490 bytes, checksum: 83e4355d0adaedd8030572d2fa359ada (MD5) LICENSE.txt: 4220 bytes, checksum: d1b2e08322b3f1dccb3fc48642eab55a (MD5) Previous issue date: 2020-11-25","Embargo set by: Seth Robbins for item 117196 Lift date: 2023-03-05T21:40:52Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 117196 Lift date: 2023-03-05T21:43:00Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Determining the beneficial properties of bovine amniotic membrane and its efficacy as a therapy for corneal healing"]}]}],"canonical_facts":{"dc:contributor":["Flaws, Jodi A","da Costa Martins, Bianca","Mahoney, Megan M","Fleming, Kathryn M"],"dc:creator":["Capistrano Da Silva, Erotides"],"dc:date":["2021-03-05T21:40:43Z","2023-03-05T21:43:00Z","2020-11-25","2020-12"],"dc:description":["Corneal ulceration, or ulcerative keratitis, is one of the most common ocular diseases observed in dogs and horses, particularly after trauma. Superficial corneal ulcers can heal without complications in a noninfected environment, but severe ulcerative keratitis can progress rapidly and lead to impaired vision and loss of the eye. Corneal healing is modulated by complex signaling pathways which involve proteinases, proteinase inhibitors, growth factors, and pro- and anti-inflammatory cytokines. For adequate corneal healing, it is necessary to have a well-balanced interaction between proteinases and their inhibitors, as well as anti-angiogenic and angiogenic factors. Therefore, keeping this balance should be the target approach in treating ulcerative keratitis. Some medications can alter the ocular microenvironment and help keep this balance, improving corneal healing. In this dissertation work, bovine amniotic membrane homogenate (BAMH) has been investigated to evaluate its efficacy as therapy during corneal healing. The first step was to confirm if BAMH contains tissue inhibitor of metalloproteinases (TIMPs), the most effective metalloproteinase inhibitors in the cornea, and if BAMH is able to inhibit metalloproteinases (MMPs) -2 and -9, which are detrimental when in excess. After confirming these properties in vitro, BAMH effectiveness during healing process was evaluated in an ex vivo wounded cornea model, topically applying the BAMH and comparing its effect with EDTA and a control. The BAMH was able to reduce corneal healing time significantly when compared to the controls, suggesting that it is a promising treatment for stromal and epithelial ulcers. Subsequently, the next step was to develop a protein profile of the bovine amniotic membrane (BAM) to understand how BAMH could potentially influence corneal ulcer therapy. To do so, BAM proteins were identified using high-throughput proteomic analysis. The results revealed that many of the expressed proteins in the BAM were also present in the cornea and involved in several biological and molecular functions. In conclusion, BAMH appears to be a promising treatment for corneal ulcers, presenting TIMP -1, TIMP -2 and TIMP -3, inhibiting MMP-2 and MMP-9 in vitro, accelerating the corneal healing time, and presenting numerous beneficial proteins important for corneal health and function.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2022-12-01","The student, Erotides Capistrano Da Silva, accepted the attached license on 2020-11-18 at 14:11.","The student, Erotides Capistrano Da Silva, submitted this Dissertation for approval on 2020-11-18 at 14:28.","This Dissertation was approved for publication on 2020-11-25 at 11:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #15903 on 2021-03-04 at 16:19:33","Made available in DSpace on 2021-03-05T21:40:43Z (GMT). No. of bitstreams: 2 CAPISTRANODASILVA-DISSERTATION-2020.pdf: 2520490 bytes, checksum: 83e4355d0adaedd8030572d2fa359ada (MD5) LICENSE.txt: 4220 bytes, checksum: d1b2e08322b3f1dccb3fc48642eab55a (MD5) Previous issue date: 2020-11-25","Embargo set by: Seth Robbins for item 117196 Lift date: 2023-03-05T21:40:52Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 117196 Lift date: 2023-03-05T21:43:00Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/109492"],"dc:language":["en"],"dc:rights":["Copyright 2020 Erotides Capistrano Da Silva"],"dc:subject":["Bovine amniotic membrane, homogenate, metalloproteinases (MMPs), TIMPs, corneal healing, proteomic composition, ex vivo corneas, in vitro MMPs -2 and -9 reductive activities"],"dc:title":["Determining the beneficial properties of bovine amniotic membrane and its efficacy as a therapy for corneal healing"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["VMS - Comparative Biosciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:50Z"}