https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/109471
type: CreativeWork
name: Evaluation of the precision-fed rooster assay for detecting effects of exogenous carbohydrases and adaptation diet on true metabolizable energy and cecal microbial ecology, short-chain fatty acid profile, and enzyme activity
description: Five experiments were conducted to evaluate the precision-fed rooster assay for its ability to detect effects of supplemental carbohydrase enzymes on nitrogen-corrected true metabolizable energy (TMEn) of feed ingredients for poultry. Experiment 1 evaluated six combinations of various α-galactosidase and xylanase levels on TMEn of a corn/soybean meal diet, while Experiment 2 evaluated two levels of a β-glucanase and xylanase on TMEn of pearled barley and rye diets, respectively. Two levels of a multi-carbohydrase combination were also evaluated for both grains. In both experiments, Single Comb White Leghorn roosters were fasted for 26 h and then crop intubated with either 25 or 30 g of the test diets. Excreta were collected quantitatively for 48 h after feeding. In Experiment 1, all carbohydrase combinations either numerically or significantly (P < 0.05) increased TMEn of the corn/soybean meal diet and the mean increase for the enzyme treatments was 66 kcal/kg DM compared with the control diet containing no added enzymes. In Experiment 2, both β-glucanase and the multi-carbohydrase combination significantly (P < 0.05) increased TMEn of the 100% barley diet, while the xylanase and multi-carbohydrase combination either numerically or significantly (P < 0.05) increased TMEn of the 100% rye diet. Overall, results of Experiments 1 and 2 indicated that the precision-fed rooster assay can detect effects of carbohydrase enzymes on TMEn of diets containing corn/soybean meal, pearled barley, and/or rye. Experiments 3-5 evaluated the hypothesis that TMEn of barley and rye diets may be influenced by type of pre-trial adaptation diet and exogenous carbohydrases. Single Comb White Leghorn roosters were fed adaptation diets based on corn/soybean meal, barley/soybean meal with and without β-glucanase, and/or rye/corn/soybean meal with and without xylanase for approximately 4 wk. After the adaptation period for Experiments 3 and 4, TMEn was determined for 100% barley or 100% rye die…
abstract: Five experiments were conducted to evaluate the precision-fed rooster assay for its ability to detect effects of supplemental carbohydrase enzymes on nitrogen-corrected true metabolizable energy (TMEn) of feed ingredients for poultry. Experiment 1 evaluated six combinations of various α-galactosidase and xylanase levels on TMEn of a corn/soybean meal diet, while Experiment 2 evaluated two levels of a β-glucanase and xylanase on TMEn of pearled barley and rye diets, respectively. Two levels of a multi-carbohydrase combination were also evaluated for both grains. In both experiments, Single Comb White Leghorn roosters were fasted for 26 h and then crop intubated with either 25 or 30 g of the test diets. Excreta were collected quantitatively for 48 h after feeding. In Experiment 1, all carbohydrase combinations either numerically or significantly (P < 0.05) increased TMEn of the corn/soybean meal diet and the mean increase for the enzyme treatments was 66 kcal/kg DM compared with the control diet containing no added enzymes. In Experiment 2, both β-glucanase and the multi-carbohydrase combination significantly (P < 0.05) increased TMEn of the 100% barley diet, while the xylanase and multi-carbohydrase combination either numerically or significantly (P < 0.05) increased TMEn of the 100% rye diet. Overall, results of Experiments 1 and 2 indicated that the precision-fed rooster assay can detect effects of carbohydrase enzymes on TMEn of diets containing corn/soybean meal, pearled barley, and/or rye. Experiments 3-5 evaluated the hypothesis that TMEn of barley and rye diets may be influenced by type of pre-trial adaptation diet and exogenous carbohydrases. Single Comb White Leghorn roosters were fed adaptation diets based on corn/soybean meal, barley/soybean meal with and without β-glucanase, and/or rye/corn/soybean meal with and without xylanase for approximately 4 wk. After the adaptation period for Experiments 3 and 4, TMEn was determined for 100% barley or 100% rye die…
author: Anderson, Annalise Grace
language: en
keywords: Carbohydrase, β-glucanase, xylanase, barley, rye, enzyme.
date published: 2021-03-05T21:40:33Z
date modified: 2026-07-22T22:24:50Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/109471