Understanding the intrinsic antibiotic resistance in Lysobacter enzymogenes, a ubiquitous environmental bacterium
type: CreativeWork
description: Antibiotics are one of the most important tools in modern medicine. Unfortunately, due to frequent use of antibiotics in clinical and agricultural areas, more bacterial strains in diverse environmental settings have been found to be resistant to many antibiotics, resulting in multidrug resistance (MDR). Increased understanding of antibiotic resistance in environmental bacteria provides new targets for discovery of novel therapeutics. However, little is known about the antibiotic resistance of the vast majority of environmental bacteria. The ubiquitous Gram-negative Lysobacter bacteria, as promising biocontrol agents, confer intrinsic antibiotic resistance and could be used as a model in understanding antibiotic resistance in non-clinical environments. First, to fill a critical knowledge gap in the prevalence and diversity of MDR in Lysobacter, we compared two strains of Lysobacter species, i.e., LeC3 and LaATCC29479, and determined their resistance to 12 common antibiotics. Our results revealed that both strains exhibited high level of MDR, and in comparison to LaATCC29479, LeC3 exhibited even higher levels of resistance against most antibiotics tested compared to LaATCC29479. Comparative resistomic analyses identified the core resistome of Lysobacter and also revealed potential genes related to the antibiotic resistance profiles in LeC3 and LaATCC29479. Second, screening two Tn5 transposon mutant libraries of LeC3 recovered 12 and 19 mutants with decreased ampicillin and spectinomycin resistance, respectively. Further characterization of selected mutants showed that cell permeability, β-lactamase activity and transport contributed to high level of resistance to ampicillin in LeC3; whereas high level of resistance to spectinomycin in LeC3 was mostly due to its ribosomal RNA target as well as related to cell-wall recycling and purine biosynthesis. Third, we demonstrated that the post-transcriptional regulator CsrA not only conferred MDR, but also contributed to bioco…
abstract: Antibiotics are one of the most important tools in modern medicine. Unfortunately, due to frequent use of antibiotics in clinical and agricultural areas, more bacterial strains in diverse environmental settings have been found to be resistant to many antibiotics, resulting in multidrug resistance (MDR). Increased understanding of antibiotic resistance in environmental bacteria provides new targets for discovery of novel therapeutics. However, little is known about the antibiotic resistance of the vast majority of environmental bacteria. The ubiquitous Gram-negative Lysobacter bacteria, as promising biocontrol agents, confer intrinsic antibiotic resistance and could be used as a model in understanding antibiotic resistance in non-clinical environments. First, to fill a critical knowledge gap in the prevalence and diversity of MDR in Lysobacter, we compared two strains of Lysobacter species, i.e., LeC3 and LaATCC29479, and determined their resistance to 12 common antibiotics. Our results revealed that both strains exhibited high level of MDR, and in comparison to LaATCC29479, LeC3 exhibited even higher levels of resistance against most antibiotics tested compared to LaATCC29479. Comparative resistomic analyses identified the core resistome of Lysobacter and also revealed potential genes related to the antibiotic resistance profiles in LeC3 and LaATCC29479. Second, screening two Tn5 transposon mutant libraries of LeC3 recovered 12 and 19 mutants with decreased ampicillin and spectinomycin resistance, respectively. Further characterization of selected mutants showed that cell permeability, β-lactamase activity and transport contributed to high level of resistance to ampicillin in LeC3; whereas high level of resistance to spectinomycin in LeC3 was mostly due to its ribosomal RNA target as well as related to cell-wall recycling and purine biosynthesis. Third, we demonstrated that the post-transcriptional regulator CsrA not only conferred MDR, but also contributed to bioco…
author: Yu, Menghao
language: en
keywords: Lysobacter, Intrinsic resistance, Ampicillin, Spectinomycin, CsrA
date published: 2020-10-07T22:48:10Z
date modified: 2026-07-22T22:24:48Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/108670