{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/108525"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/108525","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Nanoparticle effects on cell migration","abstract":"It has been found that gold nanoparticles (Au NPs) are excellent materials for biological applications such as diagnosis and drug delivery, which makes it crucial to understand the interface between gold nanoparticles and cells. Previous work has shown that gold nanoparticles can affect cellular migration, even if outside cells. However, the fundamental of interaction between Au NPs and cells has remained unclear. The most accepted mechanism currently is that by sequestering biomolecules from the environment nanoparticles can change the local molecular concentration, and thus influence cell behavior. Chemotaxis is the movement of cells in response to a chemical stimulus. Chemoattractants are inorganic and organic substances, usually proteins, that create a concentration gradient that attracts cells to move towards them. Chemoattractants are known to be involved in immune responses and wound healing processes in eukaryotic cells. With the presence of chemoattractants, cells move directionally towards the infected sites or wound. Similar processes exist in wound healing. To study the intervention of gold nanoparticles in chemotaxis, two systems were chosen. The first system that was examined in the human monocytes THP-1 cells. As a type of monocyte, THP-1 is known to migrate in response peptide formyl peptides. Another cell/chemoattractant pair that was examined was human dermal fibroblasts (HDF) and platelet-derived growth factor (PDGF). Cells were cultured and placed into 3D collagen gel in a specialized chemotaxis slide, in which a steady chemoattractant concentration was produced. Cell migration was recorded through bright-field optical microscopy. Gold nanospheres with different surface chemistries were introduced into the system. Surface chemistries include cationic PDADMAC, and anionic PSS and citrate. The hypothesis under consideration was that chemoattractant would adsorb mostly strongly to the nanoparticle of the opposite charge, thus affecting chemotaxis. A set of experiments was designed to test this hypothesis.","abstract_html":"It has been found that gold nanoparticles (Au NPs) are excellent materials for biological applications such as diagnosis and drug delivery, which makes it crucial to understand the interface between gold nanoparticles and cells. Previous work has shown that gold nanoparticles can affect cellular migration, even if outside cells. However, the fundamental of interaction between Au NPs and cells has remained unclear. The most accepted mechanism currently is that by sequestering biomolecules from the environment nanoparticles can change the local molecular concentration, and thus influence cell behavior. Chemotaxis is the movement of cells in response to a chemical stimulus. Chemoattractants are inorganic and organic substances, usually proteins, that create a concentration gradient that attracts cells to move towards them. Chemoattractants are known to be involved in immune responses and wound healing processes in eukaryotic cells. With the presence of chemoattractants, cells move directionally towards the infected sites or wound. Similar processes exist in wound healing. To study the intervention of gold nanoparticles in chemotaxis, two systems were chosen. The first system that was examined in the human monocytes THP-1 cells. As a type of monocyte, THP-1 is known to migrate in response peptide formyl peptides. Another cell/chemoattractant pair that was examined was human dermal fibroblasts (HDF) and platelet-derived growth factor (PDGF). Cells were cultured and placed into 3D collagen gel in a specialized chemotaxis slide, in which a steady chemoattractant concentration was produced. Cell migration was recorded through bright-field optical microscopy. Gold nanospheres with different surface chemistries were introduced into the system. Surface chemistries include cationic PDADMAC, and anionic PSS and citrate. The hypothesis under consideration was that chemoattractant would adsorb mostly strongly to the nanoparticle of the opposite charge, thus affecting chemotaxis. A set of experiments was designed to test this hypothesis.","abstract_has_math":false,"creators":["Zhang, Yishu"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Murphy, Catherine Jones"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-10-07T21:00:06Z","date_published":"2020-10-07T21:00:06Z","updated_at":"2026-07-22T22:24:48Z","subjects":["Gold nanoparticles","chemotaxis","cell migration"],"languages":["en"],"rights":["Copyright 2020 Yishu Zhang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/108525","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Murphy, Catherine Jones"]},{"key":"dc:creator","label":"Author","values":["Zhang, Yishu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2020-10-07T21:00:06Z","2020-07-21","2020-08"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Gold nanoparticles","chemotaxis","cell migration"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2020 Yishu Zhang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/108525"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["It has been found that gold nanoparticles (Au NPs) are excellent materials for biological applications such as diagnosis and drug delivery, which makes it crucial to understand the interface between gold nanoparticles and cells. Previous work has shown that gold nanoparticles can affect cellular migration, even if outside cells. However, the fundamental of interaction between Au NPs and cells has remained unclear. The most accepted mechanism currently is that by sequestering biomolecules from the environment nanoparticles can change the local molecular concentration, and thus influence cell behavior. Chemotaxis is the movement of cells in response to a chemical stimulus. Chemoattractants are inorganic and organic substances, usually proteins, that create a concentration gradient that attracts cells to move towards them. Chemoattractants are known to be involved in immune responses and wound healing processes in eukaryotic cells. With the presence of chemoattractants, cells move directionally towards the infected sites or wound. Similar processes exist in wound healing. To study the intervention of gold nanoparticles in chemotaxis, two systems were chosen. The first system that was examined in the human monocytes THP-1 cells. As a type of monocyte, THP-1 is known to migrate in response peptide formyl peptides. Another cell/chemoattractant pair that was examined was human dermal fibroblasts (HDF) and platelet-derived growth factor (PDGF). Cells were cultured and placed into 3D collagen gel in a specialized chemotaxis slide, in which a steady chemoattractant concentration was produced. Cell migration was recorded through bright-field optical microscopy. Gold nanospheres with different surface chemistries were introduced into the system. Surface chemistries include cationic PDADMAC, and anionic PSS and citrate. The hypothesis under consideration was that chemoattractant would adsorb mostly strongly to the nanoparticle of the opposite charge, thus affecting chemotaxis. A set of experiments was designed to test this hypothesis.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2020-10-02 without embargo terms","The student, Yishu Zhang, accepted the attached license on 2020-07-21 at 11:27.","The student, Yishu Zhang, submitted this Thesis for approval on 2020-07-21 at 11:43.","This Thesis was approved for publication on 2020-07-21 at 17:07.","DSpace SAF Submission Ingestion Package generated from Vireo submission #15706 on 2020-10-02 at 15:15:00","Made available in DSpace on 2020-10-07T21:00:06Z (GMT). No. of bitstreams: 2 ZHANG-THESIS-2020.pdf: 2050811 bytes, checksum: cdd42ff81011034eb0d78bf187a37c9f (MD5) LICENSE.txt: 4208 bytes, checksum: 00285e1bdd3b21fc53b2b342e82c43f0 (MD5) Previous issue date: 2020-07-21"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Nanoparticle effects on cell migration"]}]}],"canonical_facts":{"dc:contributor":["Murphy, Catherine Jones"],"dc:creator":["Zhang, Yishu"],"dc:date":["2020-10-07T21:00:06Z","2020-07-21","2020-08"],"dc:description":["It has been found that gold nanoparticles (Au NPs) are excellent materials for biological applications such as diagnosis and drug delivery, which makes it crucial to understand the interface between gold nanoparticles and cells. Previous work has shown that gold nanoparticles can affect cellular migration, even if outside cells. However, the fundamental of interaction between Au NPs and cells has remained unclear. The most accepted mechanism currently is that by sequestering biomolecules from the environment nanoparticles can change the local molecular concentration, and thus influence cell behavior. Chemotaxis is the movement of cells in response to a chemical stimulus. Chemoattractants are inorganic and organic substances, usually proteins, that create a concentration gradient that attracts cells to move towards them. Chemoattractants are known to be involved in immune responses and wound healing processes in eukaryotic cells. With the presence of chemoattractants, cells move directionally towards the infected sites or wound. Similar processes exist in wound healing. To study the intervention of gold nanoparticles in chemotaxis, two systems were chosen. The first system that was examined in the human monocytes THP-1 cells. As a type of monocyte, THP-1 is known to migrate in response peptide formyl peptides. Another cell/chemoattractant pair that was examined was human dermal fibroblasts (HDF) and platelet-derived growth factor (PDGF). Cells were cultured and placed into 3D collagen gel in a specialized chemotaxis slide, in which a steady chemoattractant concentration was produced. Cell migration was recorded through bright-field optical microscopy. Gold nanospheres with different surface chemistries were introduced into the system. Surface chemistries include cationic PDADMAC, and anionic PSS and citrate. The hypothesis under consideration was that chemoattractant would adsorb mostly strongly to the nanoparticle of the opposite charge, thus affecting chemotaxis. A set of experiments was designed to test this hypothesis.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2020-10-02 without embargo terms","The student, Yishu Zhang, accepted the attached license on 2020-07-21 at 11:27.","The student, Yishu Zhang, submitted this Thesis for approval on 2020-07-21 at 11:43.","This Thesis was approved for publication on 2020-07-21 at 17:07.","DSpace SAF Submission Ingestion Package generated from Vireo submission #15706 on 2020-10-02 at 15:15:00","Made available in DSpace on 2020-10-07T21:00:06Z (GMT). No. of bitstreams: 2 ZHANG-THESIS-2020.pdf: 2050811 bytes, checksum: cdd42ff81011034eb0d78bf187a37c9f (MD5) LICENSE.txt: 4208 bytes, checksum: 00285e1bdd3b21fc53b2b342e82c43f0 (MD5) Previous issue date: 2020-07-21"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/108525"],"dc:language":["en"],"dc:rights":["Copyright 2020 Yishu Zhang"],"dc:subject":["Gold nanoparticles","chemotaxis","cell migration"],"dc:title":["Nanoparticle effects on cell migration"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:48Z"}