{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/108487"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/108487","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Chemical probes for studying cyclooxygenase-2 and nitric oxide in living systems","abstract":"Molecular imaging enables the direct detection of analytes and biomolecular species within their native biological environment. Although the field derives from diagnostic biomedical imaging, there has been a significant shift over the past couple decades towards using imaging to evaluate and discover biology. In general, molecular imaging relies on the development of chemical or biochemical tools that accumulate at the site of interest or under undergo a selective, observable change following target engagement. Activity-based sensing is a powerful expansion of molecular imaging because it measures chemical reactivity rather than concentration. Chapter 1 serves as an introduction to molecular imaging with a historical tone. It also defines and highlights key examples of binding-based and activity-based sensing probes to contextualize the following chapters. Chapter 2 discusses the design and validation of a fluorescent probe for detecting cyclooxygenase-2 activity with live cells, as well as the discovery of oxygen-dependent regulation that is not observed on the protein expression level. Chapter 3 summarizes our progress towards the development of photoacoustic probes for imaging nitric oxide within live animals. Topics include the preparation of a photoacoustic probe for imaging nitric oxide in a small animal model of inflammation, the optimization of the aza-BODIPY dye platform to detect cancer-derived nitric oxide, and progress towards a multimodal dye platform for photoacoustic and fluorescence imaging.","abstract_html":"Molecular imaging enables the direct detection of analytes and biomolecular species within their native biological environment. Although the field derives from diagnostic biomedical imaging, there has been a significant shift over the past couple decades towards using imaging to evaluate and discover biology. In general, molecular imaging relies on the development of chemical or biochemical tools that accumulate at the site of interest or under undergo a selective, observable change following target engagement. Activity-based sensing is a powerful expansion of molecular imaging because it measures chemical reactivity rather than concentration. Chapter 1 serves as an introduction to molecular imaging with a historical tone. It also defines and highlights key examples of binding-based and activity-based sensing probes to contextualize the following chapters. Chapter 2 discusses the design and validation of a fluorescent probe for detecting cyclooxygenase-2 activity with live cells, as well as the discovery of oxygen-dependent regulation that is not observed on the protein expression level. Chapter 3 summarizes our progress towards the development of photoacoustic probes for imaging nitric oxide within live animals. Topics include the preparation of a photoacoustic probe for imaging nitric oxide in a small animal model of inflammation, the optimization of the aza-BODIPY dye platform to detect cancer-derived nitric oxide, and progress towards a multimodal dye platform for photoacoustic and fluorescence imaging.","abstract_has_math":false,"creators":["Reinhardt, Christopher J."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Chan, Jefferson","Gerlt, John A.","Hergenrother, Paul J.","van der Donk, Wilfred A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-10-07T20:59:51Z","date_published":"2020-10-07T20:59:51Z","updated_at":"2026-07-22T22:24:48Z","subjects":["molecular imaging, reaction-based chemical probes, activity-based sensing, cyclooxygenase, nitric oxide, fluorescence imaging, photoacoustic imaging"],"languages":["en"],"rights":["Copyright 2020 Christopher J. Reinhardt"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/108487","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Chan, Jefferson","Gerlt, John A.","Hergenrother, Paul J.","van der Donk, Wilfred A."]},{"key":"dc:creator","label":"Author","values":["Reinhardt, Christopher J."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2020-10-07T20:59:51Z","2020-07-16","2020-08"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["molecular imaging, reaction-based chemical probes, activity-based sensing, cyclooxygenase, nitric oxide, fluorescence imaging, photoacoustic imaging"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2020 Christopher J. 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Chapter 1 serves as an introduction to molecular imaging with a historical tone. It also defines and highlights key examples of binding-based and activity-based sensing probes to contextualize the following chapters. Chapter 2 discusses the design and validation of a fluorescent probe for detecting cyclooxygenase-2 activity with live cells, as well as the discovery of oxygen-dependent regulation that is not observed on the protein expression level. Chapter 3 summarizes our progress towards the development of photoacoustic probes for imaging nitric oxide within live animals. Topics include the preparation of a photoacoustic probe for imaging nitric oxide in a small animal model of inflammation, the optimization of the aza-BODIPY dye platform to detect cancer-derived nitric oxide, and progress towards a multimodal dye platform for photoacoustic and fluorescence imaging.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2020-10-02 without embargo terms","The student, Christopher Reinhardt, accepted the attached license on 2020-07-13 at 21:26.","The student, Christopher Reinhardt, submitted this Dissertation for approval on 2020-07-13 at 21:31.","This Dissertation was approved for publication on 2020-07-16 at 07:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #15598 on 2020-10-02 at 15:13:16","Made available in DSpace on 2020-10-07T20:59:51Z (GMT). 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Although the field derives from diagnostic biomedical imaging, there has been a significant shift over the past couple decades towards using imaging to evaluate and discover biology. In general, molecular imaging relies on the development of chemical or biochemical tools that accumulate at the site of interest or under undergo a selective, observable change following target engagement. Activity-based sensing is a powerful expansion of molecular imaging because it measures chemical reactivity rather than concentration. Chapter 1 serves as an introduction to molecular imaging with a historical tone. It also defines and highlights key examples of binding-based and activity-based sensing probes to contextualize the following chapters. Chapter 2 discusses the design and validation of a fluorescent probe for detecting cyclooxygenase-2 activity with live cells, as well as the discovery of oxygen-dependent regulation that is not observed on the protein expression level. Chapter 3 summarizes our progress towards the development of photoacoustic probes for imaging nitric oxide within live animals. Topics include the preparation of a photoacoustic probe for imaging nitric oxide in a small animal model of inflammation, the optimization of the aza-BODIPY dye platform to detect cancer-derived nitric oxide, and progress towards a multimodal dye platform for photoacoustic and fluorescence imaging.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2020-10-02 without embargo terms","The student, Christopher Reinhardt, accepted the attached license on 2020-07-13 at 21:26.","The student, Christopher Reinhardt, submitted this Dissertation for approval on 2020-07-13 at 21:31.","This Dissertation was approved for publication on 2020-07-16 at 07:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #15598 on 2020-10-02 at 15:13:16","Made available in DSpace on 2020-10-07T20:59:51Z (GMT). No. of bitstreams: 3 REINHARDT-DISSERTATION-2020.pdf: 34786786 bytes, checksum: 7176db1b81f161c74443d316f62df86c (MD5) LICENSE.txt: 4218 bytes, checksum: ce3a38288ccceab46e9be34afcb47df0 (MD5) PROQUEST_LICENSE.txt: 4564 bytes, checksum: a9b58cdcc3fdd23cd1d3488ef4bcb860 (MD5) Previous issue date: 2020-07-16"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/108487"],"dc:language":["en"],"dc:rights":["Copyright 2020 Christopher J. Reinhardt"],"dc:subject":["molecular imaging, reaction-based chemical probes, activity-based sensing, cyclooxygenase, nitric oxide, fluorescence imaging, photoacoustic imaging"],"dc:title":["Chemical probes for studying cyclooxygenase-2 and nitric oxide in living systems"],"dc:type":["text","Thesis"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:48Z"}