{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/106423"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/106423","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Cell culture platforms with cell-cell adhesive ligands for biomedical applications","abstract":"Cell behavior is regulated by a number of different mechanical and chemical cues within the extracellular microenvironment, including the surrounding matrix and neighboring cells. Although biomaterials have been engineered to present several adhesion molecules that mimic cell-extracellular matrix interactions, many materials do not consider the effects of cell-cell interactions. One such molecule is N-cadherin, a cell-cell adhesion protein which is expressed on both MSCs and neurons. N-cadherin regulates cytoskeleton organization, mechanotransduction, paracrine function, stem cell development, and neuronal growth. This molecule is an ideal candidate that can be incorporated into biomaterials to mimic cell-cell interactions. Recent efforts incorporated N-cadherin as well as other cell-cell adhesion proteins in hydrogel systems. However, the design of materials that effectively mimic N-cadherin interactions is not well-understood. With this in mind, my doctoral research examined the role of N-cadherin in modulating cell behaviors and the exploitation of these findings to design materials that regulate cell functions. Chapter 2 investigates the impact of different N-cadherin fragments on mesenchymal stem cell (MSC) mechanosensing and paracrine function. Chapter 3 explores how recombinant N-cadherin protein coated on different surfaces can instruct the formation of neural networks. Finally, Chapter 4 investigates the influence of N-cadherin on the secretion of exosomes, and their effects on stem cell differentiation and neuronal cultures. Together, these studies present the framework for building biomaterials that mimic cadherin-mediated cell-cell interactions in order to study stem cell mechanotransduction, to enhance paracrine function in the context of stem cell differentiation, and to improve the culture of neurons.","abstract_html":"Cell behavior is regulated by a number of different mechanical and chemical cues within the extracellular microenvironment, including the surrounding matrix and neighboring cells. Although biomaterials have been engineered to present several adhesion molecules that mimic cell-extracellular matrix interactions, many materials do not consider the effects of cell-cell interactions. One such molecule is N-cadherin, a cell-cell adhesion protein which is expressed on both MSCs and neurons. N-cadherin regulates cytoskeleton organization, mechanotransduction, paracrine function, stem cell development, and neuronal growth. This molecule is an ideal candidate that can be incorporated into biomaterials to mimic cell-cell interactions. Recent efforts incorporated N-cadherin as well as other cell-cell adhesion proteins in hydrogel systems. However, the design of materials that effectively mimic N-cadherin interactions is not well-understood. With this in mind, my doctoral research examined the role of N-cadherin in modulating cell behaviors and the exploitation of these findings to design materials that regulate cell functions. Chapter 2 investigates the impact of different N-cadherin fragments on mesenchymal stem cell (MSC) mechanosensing and paracrine function. Chapter 3 explores how recombinant N-cadherin protein coated on different surfaces can instruct the formation of neural networks. Finally, Chapter 4 investigates the influence of N-cadherin on the secretion of exosomes, and their effects on stem cell differentiation and neuronal cultures. Together, these studies present the framework for building biomaterials that mimic cadherin-mediated cell-cell interactions in order to study stem cell mechanotransduction, to enhance paracrine function in the context of stem cell differentiation, and to improve the culture of neurons.","abstract_has_math":false,"creators":["Qin, Ellen Chin"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Materials Science & Engr","degree_department":null,"school":null,"contributors":["Kong, Hyunjoon","Braun, Paul","Leckband, Deborah","Leal, Cecelia","Chen, Qian"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-03-02T22:38:35Z","date_published":"2020-03-02T22:38:35Z","updated_at":"2026-07-22T22:24:47Z","subjects":["cadherin","stem cells","neurons","hydrogel","graphene","exosome"],"languages":["en"],"rights":["Copyright 2019 Ellen Chin Qin"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/106423","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kong, Hyunjoon","Braun, Paul","Leckband, Deborah","Leal, Cecelia","Chen, Qian"]},{"key":"dc:creator","label":"Author","values":["Qin, Ellen Chin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2020-03-02T22:38:35Z","2022-03-03T10:15:08Z","2019-12-05","2019-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Materials Science & Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["cadherin","stem cells","neurons","hydrogel","graphene","exosome"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2019 Ellen Chin Qin"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/106423"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Cell behavior is regulated by a number of different mechanical and chemical cues within the extracellular microenvironment, including the surrounding matrix and neighboring cells. Although biomaterials have been engineered to present several adhesion molecules that mimic cell-extracellular matrix interactions, many materials do not consider the effects of cell-cell interactions. One such molecule is N-cadherin, a cell-cell adhesion protein which is expressed on both MSCs and neurons. N-cadherin regulates cytoskeleton organization, mechanotransduction, paracrine function, stem cell development, and neuronal growth. This molecule is an ideal candidate that can be incorporated into biomaterials to mimic cell-cell interactions. Recent efforts incorporated N-cadherin as well as other cell-cell adhesion proteins in hydrogel systems. However, the design of materials that effectively mimic N-cadherin interactions is not well-understood. With this in mind, my doctoral research examined the role of N-cadherin in modulating cell behaviors and the exploitation of these findings to design materials that regulate cell functions. Chapter 2 investigates the impact of different N-cadherin fragments on mesenchymal stem cell (MSC) mechanosensing and paracrine function. Chapter 3 explores how recombinant N-cadherin protein coated on different surfaces can instruct the formation of neural networks. Finally, Chapter 4 investigates the influence of N-cadherin on the secretion of exosomes, and their effects on stem cell differentiation and neuronal cultures. Together, these studies present the framework for building biomaterials that mimic cadherin-mediated cell-cell interactions in order to study stem cell mechanotransduction, to enhance paracrine function in the context of stem cell differentiation, and to improve the culture of neurons.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-12-01","The student, Ellen Qin, accepted the attached license on 2019-08-30 at 06:16.","The student, Ellen Qin, submitted this Dissertation for approval on 2019-08-30 at 06:27.","This Dissertation was approved for publication on 2019-12-05 at 18:16.","DSpace SAF Submission Ingestion Package generated from Vireo submission #14441 on 2020-02-28 at 17:35:15","Made available in DSpace on 2020-03-02T22:38:35Z (GMT). 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Although biomaterials have been engineered to present several adhesion molecules that mimic cell-extracellular matrix interactions, many materials do not consider the effects of cell-cell interactions. One such molecule is N-cadherin, a cell-cell adhesion protein which is expressed on both MSCs and neurons. N-cadherin regulates cytoskeleton organization, mechanotransduction, paracrine function, stem cell development, and neuronal growth. This molecule is an ideal candidate that can be incorporated into biomaterials to mimic cell-cell interactions. Recent efforts incorporated N-cadherin as well as other cell-cell adhesion proteins in hydrogel systems. However, the design of materials that effectively mimic N-cadherin interactions is not well-understood. With this in mind, my doctoral research examined the role of N-cadherin in modulating cell behaviors and the exploitation of these findings to design materials that regulate cell functions. Chapter 2 investigates the impact of different N-cadherin fragments on mesenchymal stem cell (MSC) mechanosensing and paracrine function. Chapter 3 explores how recombinant N-cadherin protein coated on different surfaces can instruct the formation of neural networks. Finally, Chapter 4 investigates the influence of N-cadherin on the secretion of exosomes, and their effects on stem cell differentiation and neuronal cultures. Together, these studies present the framework for building biomaterials that mimic cadherin-mediated cell-cell interactions in order to study stem cell mechanotransduction, to enhance paracrine function in the context of stem cell differentiation, and to improve the culture of neurons.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-12-01","The student, Ellen Qin, accepted the attached license on 2019-08-30 at 06:16.","The student, Ellen Qin, submitted this Dissertation for approval on 2019-08-30 at 06:27.","This Dissertation was approved for publication on 2019-12-05 at 18:16.","DSpace SAF Submission Ingestion Package generated from Vireo submission #14441 on 2020-02-28 at 17:35:15","Made available in DSpace on 2020-03-02T22:38:35Z (GMT). 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