{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/105957"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/105957","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Functional characterization and analysis of Carica papaya MYB24 promoter region in Arabidopsis","abstract":"This Thesis was approved for publication on 2019-07-18 at 16:35.","abstract_html":"This Thesis was approved for publication on 2019-07-18 at 16:35.","abstract_has_math":false,"creators":["Xin, Yinghui"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Plant Biology","degree_department":null,"school":null,"contributors":["Ming, Ray R"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-11-26T20:59:50Z","date_published":"2019-11-26T20:59:50Z","updated_at":"2026-07-22T22:24:45Z","subjects":["Sex determination","Papaya"],"languages":["en"],"rights":["Copyright 2019 Yinghui Xin"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/105957","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ming, Ray R"]},{"key":"dc:creator","label":"Author","values":["Xin, Yinghui"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-11-26T20:59:50Z","2021-11-27T10:15:37Z","2019-07-18","2019-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Sex determination","Papaya"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2019 Yinghui Xin"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/105957"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This Thesis was approved for publication on 2019-07-18 at 16:35.","DSpace SAF Submission Ingestion Package generated from Vireo submission #14356 on 2019-11-26 at 14:04:22","Made available in DSpace on 2019-11-26T20:59:50Z (GMT). No. of bitstreams: 2 XIN-THESIS-2019.pdf: 888606 bytes, checksum: 490cfd52b9895e8ec5e732f7c6d4853f (MD5) LICENSE.txt: 4208 bytes, checksum: afae4b4997432b7a666eff224cfcaa2b (MD5) Previous issue date: 2019-07-18","Embargo set by: Seth Robbins for item 113104 Lift date: 2021-11-26T20:59:54Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 113104 on 2021-11-27T10:15:37Z.","Papaya is a trioecious plant with three sex types: female (XX), male (XY) and hermaphrodite (XYh). It is also among limited members of plant species that have two different breeding systems, it is either dioecious, propagating with male and female or gynodioecious, propagating with hermaphrodite and female. Papaya sex chromosomes were identified, leading to the sequencing of the genome and the non-recombining regions of the X, Y, and Yh. The CpMYB24 is a promising candidate gene for the control of the sex determination and could be responsible for stamen (i.e., anther and anther filament) development. To investigate the expression profiling of CpMYB24 gene, the promoter regions of CpMYB24 gene was cloned from three sex chromosomes (X, Y, and Yh) and constructed a deletion series of reporter constructs to detect the gene expression pattern of those promoters. The results indicated that three CpMYB24 promoters were all expressed in the stamen at the late flower developmental stages in Arabidopsis thaliana. However, the GUS expression was also observed in the style of the transgenic lines driven by promoters from either X or Yh alleles of CpMYB24. Moreover, the cis-acting motif MYB1 located at 218bp upstream of CpMYB24 promoter from Y Chromosome was identified as the responsible element for the pollen-specific expression. MYB1 was detected regulating defense-related expression previously; however, the new function of MYB1 was discovered in this study to be regulating pollen-specific expression. These results confirmed the hypothesis that CpMYB24 promoters can activate expression only in reproductive organs of Arabidopsis; promoter of Y allele of CpMYB24 showed high male-specificity; and novel function of cis-regulating motif MYB1 was discovered in CpMYB24 promoter from Y chromosome.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-08-01","The student, Yinghui Xin, accepted the attached license on 2019-07-18 at 09:47.","The student, Yinghui Xin, submitted this Thesis for approval on 2019-07-18 at 09:53."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Functional characterization and analysis of Carica papaya MYB24 promoter region in Arabidopsis"]}]}],"canonical_facts":{"dc:contributor":["Ming, Ray R"],"dc:creator":["Xin, Yinghui"],"dc:date":["2019-11-26T20:59:50Z","2021-11-27T10:15:37Z","2019-07-18","2019-08"],"dc:description":["This Thesis was approved for publication on 2019-07-18 at 16:35.","DSpace SAF Submission Ingestion Package generated from Vireo submission #14356 on 2019-11-26 at 14:04:22","Made available in DSpace on 2019-11-26T20:59:50Z (GMT). No. of bitstreams: 2 XIN-THESIS-2019.pdf: 888606 bytes, checksum: 490cfd52b9895e8ec5e732f7c6d4853f (MD5) LICENSE.txt: 4208 bytes, checksum: afae4b4997432b7a666eff224cfcaa2b (MD5) Previous issue date: 2019-07-18","Embargo set by: Seth Robbins for item 113104 Lift date: 2021-11-26T20:59:54Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 113104 on 2021-11-27T10:15:37Z.","Papaya is a trioecious plant with three sex types: female (XX), male (XY) and hermaphrodite (XYh). It is also among limited members of plant species that have two different breeding systems, it is either dioecious, propagating with male and female or gynodioecious, propagating with hermaphrodite and female. Papaya sex chromosomes were identified, leading to the sequencing of the genome and the non-recombining regions of the X, Y, and Yh. The CpMYB24 is a promising candidate gene for the control of the sex determination and could be responsible for stamen (i.e., anther and anther filament) development. To investigate the expression profiling of CpMYB24 gene, the promoter regions of CpMYB24 gene was cloned from three sex chromosomes (X, Y, and Yh) and constructed a deletion series of reporter constructs to detect the gene expression pattern of those promoters. The results indicated that three CpMYB24 promoters were all expressed in the stamen at the late flower developmental stages in Arabidopsis thaliana. However, the GUS expression was also observed in the style of the transgenic lines driven by promoters from either X or Yh alleles of CpMYB24. Moreover, the cis-acting motif MYB1 located at 218bp upstream of CpMYB24 promoter from Y Chromosome was identified as the responsible element for the pollen-specific expression. MYB1 was detected regulating defense-related expression previously; however, the new function of MYB1 was discovered in this study to be regulating pollen-specific expression. These results confirmed the hypothesis that CpMYB24 promoters can activate expression only in reproductive organs of Arabidopsis; promoter of Y allele of CpMYB24 showed high male-specificity; and novel function of cis-regulating motif MYB1 was discovered in CpMYB24 promoter from Y chromosome.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-08-01","The student, Yinghui Xin, accepted the attached license on 2019-07-18 at 09:47.","The student, Yinghui Xin, submitted this Thesis for approval on 2019-07-18 at 09:53."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/105957"],"dc:language":["en"],"dc:rights":["Copyright 2019 Yinghui Xin"],"dc:subject":["Sex determination","Papaya"],"dc:title":["Functional characterization and analysis of Carica papaya MYB24 promoter region in Arabidopsis"],"dc:type":["text"],"thesis:degree_discipline":["Plant Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:45Z"}