{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/105831"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/105831","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Using model miniature ham as a high-throughput tool to screen antimicrobials targeting l. Monocytogenes","abstract":"The foodborne pathogen Listeria monocytogenes can be transmitted through eating contaminated foods, with outbreaks associated with ready-to-eat (RTE) products, such as deli ham, turkey, and hot dogs. Luncheon meats are often formulated with antimicrobial agents to limit the growth of this pathogenic microorganism. The present work develops methods to improve the current screening of antimicrobials for L. monocytogenes in deli ham. A miniature ham model was developed to minimize ham and antimicrobial use while utilizing common laboratory equipment. Growth of L. monocytogenes in the miniature ham model was compared to the traditional screening method of whole ham slices with and without an antimicrobial treatment and was confirmed to be not statistically different. An advanced statistical design, response surface methodology, was combined with the miniature ham model to efficiently screen antimicrobial treatments and combinations. Multiple Listeria spp. were utilized to screen four antimicrobials: nisin, potassium lactate sodium diacetate, lauric arginate, and bacteriophage Listex™ P100. The statistical design reduced the required amount of runs compared to traditional screening while identifying effective antimicrobial levels and antimicrobial combinations for all tested strains. In this model, nisin was identified as the most effective, regardless of the Listeria spp. Lastly, the miniature ham system was combined with a luminescence-based assay as a rapid and non-destructive preliminary screening tool. A luminescent L. monocytogenes strain was utilized to observe a correlation between traditional plate counts and light-emission from the ham surface. This was implemented into a screening of numerous antimicrobials by light-emission to distinguish between treatments that allowed a large amount of growth and treatments that did not, with cells numbers showing significant differences. Each of these models can be used to efficiently screen novel antimicrobials or compare between multiple ham formulations.","abstract_html":"The foodborne pathogen Listeria monocytogenes can be transmitted through eating contaminated foods, with outbreaks associated with ready-to-eat (RTE) products, such as deli ham, turkey, and hot dogs. Luncheon meats are often formulated with antimicrobial agents to limit the growth of this pathogenic microorganism. The present work develops methods to improve the current screening of antimicrobials for L. monocytogenes in deli ham. A miniature ham model was developed to minimize ham and antimicrobial use while utilizing common laboratory equipment. Growth of L. monocytogenes in the miniature ham model was compared to the traditional screening method of whole ham slices with and without an antimicrobial treatment and was confirmed to be not statistically different. An advanced statistical design, response surface methodology, was combined with the miniature ham model to efficiently screen antimicrobial treatments and combinations. Multiple Listeria spp. were utilized to screen four antimicrobials: nisin, potassium lactate sodium diacetate, lauric arginate, and bacteriophage Listex™ P100. The statistical design reduced the required amount of runs compared to traditional screening while identifying effective antimicrobial levels and antimicrobial combinations for all tested strains. In this model, nisin was identified as the most effective, regardless of the Listeria spp. Lastly, the miniature ham system was combined with a luminescence-based assay as a rapid and non-destructive preliminary screening tool. A luminescent L. monocytogenes strain was utilized to observe a correlation between traditional plate counts and light-emission from the ham surface. This was implemented into a screening of numerous antimicrobials by light-emission to distinguish between treatments that allowed a large amount of growth and treatments that did not, with cells numbers showing significant differences. Each of these models can be used to efficiently screen novel antimicrobials or compare between multiple ham formulations.","abstract_has_math":false,"creators":["Rezac, Shannon Deborah"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Miller, Michael J","Stasiewicz, Matthew J"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-11-26T20:49:32Z","date_published":"2019-11-26T20:49:32Z","updated_at":"2026-07-22T22:24:45Z","subjects":["Listeria","L. monocytogenes","Response Surface Methodology","Ham","Deli Meat","Luminescence","Antimicrobial"],"languages":["en"],"rights":["Copyright 2019 Shannon Rezac"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/105831","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Miller, Michael J","Stasiewicz, Matthew J"]},{"key":"dc:creator","label":"Author","values":["Rezac, Shannon Deborah"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-11-26T20:49:32Z","2021-11-27T10:15:30Z","2019-07-16","2019-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Listeria","L. monocytogenes","Response Surface Methodology","Ham","Deli Meat","Luminescence","Antimicrobial"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2019 Shannon Rezac"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/105831"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The foodborne pathogen Listeria monocytogenes can be transmitted through eating contaminated foods, with outbreaks associated with ready-to-eat (RTE) products, such as deli ham, turkey, and hot dogs. Luncheon meats are often formulated with antimicrobial agents to limit the growth of this pathogenic microorganism. The present work develops methods to improve the current screening of antimicrobials for L. monocytogenes in deli ham. A miniature ham model was developed to minimize ham and antimicrobial use while utilizing common laboratory equipment. Growth of L. monocytogenes in the miniature ham model was compared to the traditional screening method of whole ham slices with and without an antimicrobial treatment and was confirmed to be not statistically different. An advanced statistical design, response surface methodology, was combined with the miniature ham model to efficiently screen antimicrobial treatments and combinations. Multiple Listeria spp. were utilized to screen four antimicrobials: nisin, potassium lactate sodium diacetate, lauric arginate, and bacteriophage Listex™ P100. The statistical design reduced the required amount of runs compared to traditional screening while identifying effective antimicrobial levels and antimicrobial combinations for all tested strains. In this model, nisin was identified as the most effective, regardless of the Listeria spp. Lastly, the miniature ham system was combined with a luminescence-based assay as a rapid and non-destructive preliminary screening tool. A luminescent L. monocytogenes strain was utilized to observe a correlation between traditional plate counts and light-emission from the ham surface. This was implemented into a screening of numerous antimicrobials by light-emission to distinguish between treatments that allowed a large amount of growth and treatments that did not, with cells numbers showing significant differences. Each of these models can be used to efficiently screen novel antimicrobials or compare between multiple ham formulations.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2021-08-01","The student, Shannon Rezac, accepted the attached license on 2019-07-16 at 11:22.","The student, Shannon Rezac, submitted this Thesis for approval on 2019-07-16 at 11:23.","This Thesis was approved for publication on 2019-07-16 at 15:31.","DSpace SAF Submission Ingestion Package generated from Vireo submission #14340 on 2019-11-26 at 13:06:03","Made available in DSpace on 2019-11-26T20:49:32Z (GMT). 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Growth of L. monocytogenes in the miniature ham model was compared to the traditional screening method of whole ham slices with and without an antimicrobial treatment and was confirmed to be not statistically different. An advanced statistical design, response surface methodology, was combined with the miniature ham model to efficiently screen antimicrobial treatments and combinations. Multiple Listeria spp. were utilized to screen four antimicrobials: nisin, potassium lactate sodium diacetate, lauric arginate, and bacteriophage Listex™ P100. The statistical design reduced the required amount of runs compared to traditional screening while identifying effective antimicrobial levels and antimicrobial combinations for all tested strains. In this model, nisin was identified as the most effective, regardless of the Listeria spp. Lastly, the miniature ham system was combined with a luminescence-based assay as a rapid and non-destructive preliminary screening tool. A luminescent L. monocytogenes strain was utilized to observe a correlation between traditional plate counts and light-emission from the ham surface. This was implemented into a screening of numerous antimicrobials by light-emission to distinguish between treatments that allowed a large amount of growth and treatments that did not, with cells numbers showing significant differences. Each of these models can be used to efficiently screen novel antimicrobials or compare between multiple ham formulations.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2021-08-01","The student, Shannon Rezac, accepted the attached license on 2019-07-16 at 11:22.","The student, Shannon Rezac, submitted this Thesis for approval on 2019-07-16 at 11:23.","This Thesis was approved for publication on 2019-07-16 at 15:31.","DSpace SAF Submission Ingestion Package generated from Vireo submission #14340 on 2019-11-26 at 13:06:03","Made available in DSpace on 2019-11-26T20:49:32Z (GMT). No. of bitstreams: 3 REZAC-THESIS-2019.pdf: 1552811 bytes, checksum: fc93d4abb80dd4b3435961742b336132 (MD5) Thesis-Masters-Rezac 07162019 FINAL.docx: 6702729 bytes, checksum: 78e26d1309d06d23014e8b0a00fc6c9b (MD5) LICENSE.txt: 4210 bytes, checksum: 4a7f7e82b28f819d6e6f712967eb98ed (MD5) Previous issue date: 2019-07-16","Embargo set by: Seth Robbins for item 112976 Lift date: 2021-11-26T20:49:41Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 112976 on 2021-11-27T10:15:30Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/105831"],"dc:language":["en"],"dc:rights":["Copyright 2019 Shannon Rezac"],"dc:subject":["Listeria","L. monocytogenes","Response Surface Methodology","Ham","Deli Meat","Luminescence","Antimicrobial"],"dc:title":["Using model miniature ham as a high-throughput tool to screen antimicrobials targeting l. Monocytogenes"],"dc:type":["text"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:45Z"}