{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/105209"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/105209","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Bioconversion of xylose into high-value products by engineered saccharomyces cerevisiae","abstract":"The student, Stephan Lane, accepted the attached license on 2019-04-16 at 21:01.","abstract_html":"The student, Stephan Lane, accepted the attached license on 2019-04-16 at 21:01.","abstract_has_math":false,"creators":["Lane, Stephan Thomas"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Jin, Yong-Su","Stasiewicz, Matthew","Cadwallader, Keith","Rao, Christopher"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-08-23T20:47:30Z","date_published":"2019-08-23T20:47:30Z","updated_at":"2026-07-22T22:24:44Z","subjects":["xylose, yeast, Saccharomyces cerevisiae, isobutanol, gadusol, sedoheptulose, lactic acid, metabolic engineering, cellobiose, cellulosic, biofuels"],"languages":["en"],"rights":["Copyright 2019 Stephan Lane"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/105209","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Jin, Yong-Su","Stasiewicz, Matthew","Cadwallader, Keith","Rao, Christopher"]},{"key":"dc:creator","label":"Author","values":["Lane, Stephan Thomas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-08-23T20:47:30Z","2021-08-24T09:15:16Z","2019-04-18","2019-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["xylose, yeast, Saccharomyces cerevisiae, isobutanol, gadusol, sedoheptulose, lactic acid, metabolic engineering, cellobiose, cellulosic, biofuels"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2019 Stephan Lane"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/105209"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The student, Stephan Lane, accepted the attached license on 2019-04-16 at 21:01.","The student, Stephan Lane, submitted this Dissertation for approval on 2019-04-16 at 21:33.","This Dissertation was approved for publication on 2019-04-18 at 10:50.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13694 on 2019-08-22 at 16:22:23","Made available in DSpace on 2019-08-23T20:47:30Z (GMT). No. of bitstreams: 3 LANE-DISSERTATION-2019.pdf: 3305614 bytes, checksum: 8750a1380b60b42e9d81d3338ac39b9a (MD5) Lane - Dissertation - Final.docx: 3899380 bytes, checksum: f21e8c9885e09ca9121f94a8fd298a67 (MD5) LICENSE.txt: 4209 bytes, checksum: 1005f2ab6d1ce33930eee4ba43f67952 (MD5) Previous issue date: 2019-04-18","Embargo set by: Seth Robbins for item 112330 Lift date: 2021-08-23T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 112330 Lift date: 2021-08-23T20:48:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 112330 on 2021-08-24T09:15:16Z.","Significant efforts over the past few decades have focused on engineering the common brewer’s yeast Saccharomyces cerevisiae to consume xylose, the second-most abundant sugar in nature. Throughout these efforts, the goal has primarily been towards biofuels with ethanol as a target product. This research revealed that many aspects of xylose metabolism in yeast are unfavorable for production of ethanol and substantial efforts have been directed towards enhancing yeast’s limited ability to produce ethanol from xylose. With this narrow focus, many aspects of xylose metabolism have been overlooked which favor the production of non-ethanol compounds. I present here a literature review of all compounds which have been produced from xylose using engineered S. cerevisiae. Additionally, I will present personal research into developing engineering yeast strains capable of producing three compounds from xylose: isobutanol, sedoheptulose, and gadusol. Finally, I investigate the benefits of xylose metabolism towards lactic acid production and show that simultaneous co-fermentation of glucose and xylose leads to enhanced lactic acid yields. This work aims to highlight the benefits of xylose metabolism for many yeast metabolic engineering efforts and hopes to promote additional work into this fruitful area of research.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-05-01"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Bioconversion of xylose into high-value products by engineered saccharomyces cerevisiae"]}]}],"canonical_facts":{"dc:contributor":["Jin, Yong-Su","Stasiewicz, Matthew","Cadwallader, Keith","Rao, Christopher"],"dc:creator":["Lane, Stephan Thomas"],"dc:date":["2019-08-23T20:47:30Z","2021-08-24T09:15:16Z","2019-04-18","2019-05"],"dc:description":["The student, Stephan Lane, accepted the attached license on 2019-04-16 at 21:01.","The student, Stephan Lane, submitted this Dissertation for approval on 2019-04-16 at 21:33.","This Dissertation was approved for publication on 2019-04-18 at 10:50.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13694 on 2019-08-22 at 16:22:23","Made available in DSpace on 2019-08-23T20:47:30Z (GMT). No. of bitstreams: 3 LANE-DISSERTATION-2019.pdf: 3305614 bytes, checksum: 8750a1380b60b42e9d81d3338ac39b9a (MD5) Lane - Dissertation - Final.docx: 3899380 bytes, checksum: f21e8c9885e09ca9121f94a8fd298a67 (MD5) LICENSE.txt: 4209 bytes, checksum: 1005f2ab6d1ce33930eee4ba43f67952 (MD5) Previous issue date: 2019-04-18","Embargo set by: Seth Robbins for item 112330 Lift date: 2021-08-23T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 112330 Lift date: 2021-08-23T20:48:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 112330 on 2021-08-24T09:15:16Z.","Significant efforts over the past few decades have focused on engineering the common brewer’s yeast Saccharomyces cerevisiae to consume xylose, the second-most abundant sugar in nature. Throughout these efforts, the goal has primarily been towards biofuels with ethanol as a target product. This research revealed that many aspects of xylose metabolism in yeast are unfavorable for production of ethanol and substantial efforts have been directed towards enhancing yeast’s limited ability to produce ethanol from xylose. With this narrow focus, many aspects of xylose metabolism have been overlooked which favor the production of non-ethanol compounds. I present here a literature review of all compounds which have been produced from xylose using engineered S. cerevisiae. Additionally, I will present personal research into developing engineering yeast strains capable of producing three compounds from xylose: isobutanol, sedoheptulose, and gadusol. Finally, I investigate the benefits of xylose metabolism towards lactic acid production and show that simultaneous co-fermentation of glucose and xylose leads to enhanced lactic acid yields. This work aims to highlight the benefits of xylose metabolism for many yeast metabolic engineering efforts and hopes to promote additional work into this fruitful area of research.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-05-01"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/105209"],"dc:language":["en"],"dc:rights":["Copyright 2019 Stephan Lane"],"dc:subject":["xylose, yeast, Saccharomyces cerevisiae, isobutanol, gadusol, sedoheptulose, lactic acid, metabolic engineering, cellobiose, cellulosic, biofuels"],"dc:title":["Bioconversion of xylose into high-value products by engineered saccharomyces cerevisiae"],"dc:type":["text"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:44Z"}