{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/105162"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/105162","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Methodologies to promote muscle development for engineering functional skeletal muscle tissue","abstract":"The student, Eunkyung Ko, accepted the attached license on 2019-04-11 at 16:50.","abstract_html":"The student, Eunkyung Ko, accepted the attached license on 2019-04-11 at 16:50.","abstract_has_math":false,"creators":["Ko, Eunkyung"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Kong, Hyunjoon","Bashir, Rashid","Gillette, Martha L.","Saif, M. Taher"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-08-23T20:44:39Z","date_published":"2019-08-23T20:44:39Z","updated_at":"2026-07-22T22:24:44Z","subjects":["Skeletal muscle, Muscle tissue engineering, Neuromuscular Junctions, Connexin, Gap junction proteins"],"languages":["en"],"rights":["In regards to chapter 3; This chapter is adapted from Ko, E.; Yu, S. J.; Pagan-Diaz, G. J.; Mahmassani, Z.; Boppart, M. D.; Im, S. G.; Bashir, R.; Kong, H. Matrix Topography Regulates Synaptic Transmission at the Neuromuscular Junction. Advanced Science 2018, advs.201801521. Copyright Wiley-VCH Verlag GmbH & Co. KGaA. Reproduced with permission. Wiley-VCH hereby licenses back to the Contributor the following rights with respect to the final published version of the Contribution:"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/105162","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kong, Hyunjoon","Bashir, Rashid","Gillette, Martha L.","Saif, M. 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J.; Pagan-Diaz, G. J.; Mahmassani, Z.; Boppart, M. D.; Im, S. G.; Bashir, R.; Kong, H. Matrix Topography Regulates Synaptic Transmission at the Neuromuscular Junction. Advanced Science 2018, advs.201801521. Copyright Wiley-VCH Verlag GmbH & Co. KGaA. Reproduced with permission. Wiley-VCH hereby licenses back to the Contributor the following rights with respect to the final published version of the Contribution:"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/105162"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The student, Eunkyung Ko, accepted the attached license on 2019-04-11 at 16:50.","The student, Eunkyung Ko, submitted this Dissertation for approval on 2019-04-11 at 16:54.","This Dissertation was approved for publication on 2019-04-16 at 18:09.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13500 on 2019-08-22 at 16:20:45","Made available in DSpace on 2019-08-23T20:44:39Z (GMT). No. of bitstreams: 4 KO-DISSERTATION-2019.pdf: 16852982 bytes, checksum: 8702d4b1963bb20ce4c7de216531d64b (MD5) LICENSE.txt: 4205 bytes, checksum: 5fa0317d9fa900e9365eadfb04e9d322 (MD5) PROQUEST_LICENSE.txt: 4551 bytes, checksum: f6756bc01f75248a575633b21ba30069 (MD5) Permission_Wiley.png: 237679 bytes, checksum: 3f71fe6cd839da47240ac51d2d8942c3 (MD5) Previous issue date: 2019-04-16","Embargo set by: Seth Robbins for item 112281 Lift date: 2021-08-23T20:44:50Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 112281 Lift date: 2021-08-23T20:46:41Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Skeletal muscle related diseases cause loss and weakness in the muscle and affect the quality of life of the patients. Although skeletal muscle tissue can regenerate when the damage is small, a patient must receive a surgery when the volumetric loss is too large. Skeletal muscle tissue engineering aims to recapitulate the physiology and function of the natural muscle. The engineered tissue holds potential as a transplantable material that can help regeneration in vivo. In this thesis, we used different methodologies to recapitulate the natural skeletal muscle and enhance muscle development. The first part of the thesis uses physical approach to engineer functional skeletal muscle. A grooved substrate with controlled width was used to promote myogenic differentiation and enhance muscle maturity. After the mature muscle was generated, we cultured neural stem cells and let them innervate into the skeletal muscle to form a neuron-muscle interface to finally engineer a functional skeletal muscle (Chapter 3). The next study focuses on chemically stimulating the skeletal myoblasts to express more gap junction proteins and prolong the expression time. After confirming the genetic changes occurring in the muscle cells, we assembled a pump-bot device with the pre-stimulated skeletal muscle tissue engineered in a 3D ring. We compared how the fluid movement in the pump-bot was changed when the myoblasts had been chemically stimulated (Chapter 4). Lastly, we biologically modified the skeletal myoblasts to express gap junction proteins in myotubes (Chapter 5). We delivered connexin genes to the skeletal myoblasts. We examined how the presence of connexin in the cells contributes to myogenic differentiation and muscle development. Overall, the studies herein will be useful to engineer skeletal muscle for biomedical uses such as developing platforms for in vitro drug screening, engineering tissue for transplantation, and assembling a biologically actuated machinery.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-05-01","Embargo set by: Seth Robbins for item 112281 Lift date: 2021-08-23T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 112281 Lift date: 2021-08-23T20:48:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 112281 on 2021-08-24T09:15:34Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Methodologies to promote muscle development for engineering functional skeletal muscle tissue"]}]}],"canonical_facts":{"dc:contributor":["Kong, Hyunjoon","Bashir, Rashid","Gillette, Martha L.","Saif, M. Taher"],"dc:creator":["Ko, Eunkyung"],"dc:date":["2019-08-23T20:44:39Z","2021-08-24T09:15:34Z","2019-04-16","2019-05"],"dc:description":["The student, Eunkyung Ko, accepted the attached license on 2019-04-11 at 16:50.","The student, Eunkyung Ko, submitted this Dissertation for approval on 2019-04-11 at 16:54.","This Dissertation was approved for publication on 2019-04-16 at 18:09.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13500 on 2019-08-22 at 16:20:45","Made available in DSpace on 2019-08-23T20:44:39Z (GMT). No. of bitstreams: 4 KO-DISSERTATION-2019.pdf: 16852982 bytes, checksum: 8702d4b1963bb20ce4c7de216531d64b (MD5) LICENSE.txt: 4205 bytes, checksum: 5fa0317d9fa900e9365eadfb04e9d322 (MD5) PROQUEST_LICENSE.txt: 4551 bytes, checksum: f6756bc01f75248a575633b21ba30069 (MD5) Permission_Wiley.png: 237679 bytes, checksum: 3f71fe6cd839da47240ac51d2d8942c3 (MD5) Previous issue date: 2019-04-16","Embargo set by: Seth Robbins for item 112281 Lift date: 2021-08-23T20:44:50Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 112281 Lift date: 2021-08-23T20:46:41Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Skeletal muscle related diseases cause loss and weakness in the muscle and affect the quality of life of the patients. Although skeletal muscle tissue can regenerate when the damage is small, a patient must receive a surgery when the volumetric loss is too large. Skeletal muscle tissue engineering aims to recapitulate the physiology and function of the natural muscle. The engineered tissue holds potential as a transplantable material that can help regeneration in vivo. In this thesis, we used different methodologies to recapitulate the natural skeletal muscle and enhance muscle development. The first part of the thesis uses physical approach to engineer functional skeletal muscle. A grooved substrate with controlled width was used to promote myogenic differentiation and enhance muscle maturity. After the mature muscle was generated, we cultured neural stem cells and let them innervate into the skeletal muscle to form a neuron-muscle interface to finally engineer a functional skeletal muscle (Chapter 3). The next study focuses on chemically stimulating the skeletal myoblasts to express more gap junction proteins and prolong the expression time. After confirming the genetic changes occurring in the muscle cells, we assembled a pump-bot device with the pre-stimulated skeletal muscle tissue engineered in a 3D ring. We compared how the fluid movement in the pump-bot was changed when the myoblasts had been chemically stimulated (Chapter 4). Lastly, we biologically modified the skeletal myoblasts to express gap junction proteins in myotubes (Chapter 5). We delivered connexin genes to the skeletal myoblasts. We examined how the presence of connexin in the cells contributes to myogenic differentiation and muscle development. Overall, the studies herein will be useful to engineer skeletal muscle for biomedical uses such as developing platforms for in vitro drug screening, engineering tissue for transplantation, and assembling a biologically actuated machinery.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2021-05-01","Embargo set by: Seth Robbins for item 112281 Lift date: 2021-08-23T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 112281 Lift date: 2021-08-23T20:48:32Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 112281 on 2021-08-24T09:15:34Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/105162"],"dc:language":["en"],"dc:rights":["In regards to chapter 3; This chapter is adapted from Ko, E.; Yu, S. J.; Pagan-Diaz, G. J.; Mahmassani, Z.; Boppart, M. D.; Im, S. G.; Bashir, R.; Kong, H. Matrix Topography Regulates Synaptic Transmission at the Neuromuscular Junction. Advanced Science 2018, advs.201801521. Copyright Wiley-VCH Verlag GmbH & Co. KGaA. Reproduced with permission. Wiley-VCH hereby licenses back to the Contributor the following rights with respect to the final published version of the Contribution:"],"dc:subject":["Skeletal muscle, Muscle tissue engineering, Neuromuscular Junctions, Connexin, Gap junction proteins"],"dc:title":["Methodologies to promote muscle development for engineering functional skeletal muscle tissue"],"dc:type":["text"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:44Z"}