{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/105079"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/105079","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Differential gene expression analysis reveals functional roles of cryptic orange-pigmented organ in the colorless head kidney of Antarctic icefishes","abstract":"We discovered an orange-pigmented, discrete body several millimeters in size embedded within the anterior end of the colorless head kidney of the hemoglobinless Antarctic icefishes. No macroscopically visible discrete organ has previously been reported in Antarctic fishes or teleost fishes in general. To assess the tissue type and functions of this cryptic orange body, we performed RNA sequencing and analyzed the orange body transcriptome relative to the surrounding head kidney tissue. We found 5,263 differentially expressed genes in the orange body, of which 4,624 genes were up-regulated and 639 down-regulated relative to the head kidney tissue. Differential gene expression analyses detected the enrichment of two main biological pathways: steroid hormone production and proline biosynthesis. The steroidogenic activities of the orange body indicate it performs functional roles of the interrenal tissue cells – the teleost homolog of the tetrapod adrenal cortex but are normally dispersed among the teleost head kidney tissue. Transcription of many cytochrome P450 enzymes participating in steroidogenesis were upregulated, potentially leading to greater levels of ROS and peroxidation of the lipid-rich steroid producing orange body cells. Proline is known to act as an antioxidant, thus, the detected enrichment in proline synthesis may be related to antioxidation defense mechanism, conferring protection against lipid peroxidation in steroid rich orange body cells.","abstract_html":"We discovered an orange-pigmented, discrete body several millimeters in size embedded within the anterior end of the colorless head kidney of the hemoglobinless Antarctic icefishes. No macroscopically visible discrete organ has previously been reported in Antarctic fishes or teleost fishes in general. To assess the tissue type and functions of this cryptic orange body, we performed RNA sequencing and analyzed the orange body transcriptome relative to the surrounding head kidney tissue. We found 5,263 differentially expressed genes in the orange body, of which 4,624 genes were up-regulated and 639 down-regulated relative to the head kidney tissue. Differential gene expression analyses detected the enrichment of two main biological pathways: steroid hormone production and proline biosynthesis. The steroidogenic activities of the orange body indicate it performs functional roles of the interrenal tissue cells – the teleost homolog of the tetrapod adrenal cortex but are normally dispersed among the teleost head kidney tissue. Transcription of many cytochrome P450 enzymes participating in steroidogenesis were upregulated, potentially leading to greater levels of ROS and peroxidation of the lipid-rich steroid producing orange body cells. Proline is known to act as an antioxidant, thus, the detected enrichment in proline synthesis may be related to antioxidation defense mechanism, conferring protection against lipid peroxidation in steroid rich orange body cells.","abstract_has_math":false,"creators":["Yoon, Eun Hye"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Ecology, Ethology, and Evolution","degree_department":null,"school":null,"contributors":["Cheng, Chi-Hing C.","DeVries, Arthur","Catchen, Julian"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-08-23T20:36:07Z","date_published":"2019-08-23T20:36:07Z","updated_at":"2026-07-22T22:24:44Z","subjects":["RNA-Seq","Antarctic icefish","Head kidney","Interrenal tissue","Proline biosynthesis","Transcriptome"],"languages":["en"],"rights":["Copyright 2019 Eun Hye Yoon"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/105079","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cheng, Chi-Hing C.","DeVries, Arthur","Catchen, Julian"]},{"key":"dc:creator","label":"Author","values":["Yoon, Eun Hye"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-08-23T20:36:07Z","2021-08-24T09:15:20Z","2019-04-24","2019-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Ecology, Ethology, and Evolution"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["RNA-Seq","Antarctic icefish","Head kidney","Interrenal tissue","Proline biosynthesis","Transcriptome"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2019 Eun Hye Yoon"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/105079"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["We discovered an orange-pigmented, discrete body several millimeters in size embedded within the anterior end of the colorless head kidney of the hemoglobinless Antarctic icefishes. No macroscopically visible discrete organ has previously been reported in Antarctic fishes or teleost fishes in general. To assess the tissue type and functions of this cryptic orange body, we performed RNA sequencing and analyzed the orange body transcriptome relative to the surrounding head kidney tissue. We found 5,263 differentially expressed genes in the orange body, of which 4,624 genes were up-regulated and 639 down-regulated relative to the head kidney tissue. Differential gene expression analyses detected the enrichment of two main biological pathways: steroid hormone production and proline biosynthesis. The steroidogenic activities of the orange body indicate it performs functional roles of the interrenal tissue cells – the teleost homolog of the tetrapod adrenal cortex but are normally dispersed among the teleost head kidney tissue. Transcription of many cytochrome P450 enzymes participating in steroidogenesis were upregulated, potentially leading to greater levels of ROS and peroxidation of the lipid-rich steroid producing orange body cells. Proline is known to act as an antioxidant, thus, the detected enrichment in proline synthesis may be related to antioxidation defense mechanism, conferring protection against lipid peroxidation in steroid rich orange body cells.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2021-05-01","The student, Eun Hye Yoon, accepted the attached license on 2019-04-23 at 23:17.","The student, Eun Hye Yoon, submitted this Thesis for approval on 2019-04-23 at 23:34.","This Thesis was approved for publication on 2019-04-24 at 13:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13850 on 2019-08-22 at 15:07:53","Made available in DSpace on 2019-08-23T20:36:07Z (GMT). No. of bitstreams: 2 YOON-THESIS-2019.pdf: 17082804 bytes, checksum: b7a8b01a928d161a3dc2deffebccd00b (MD5) LICENSE.txt: 4210 bytes, checksum: 1a8d694365c4b9326ff9f2d844b12935 (MD5) Previous issue date: 2019-04-24","Embargo set by: Seth Robbins for item 112198 Lift date: 2021-08-23T20:36:18Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 112198 on 2021-08-24T09:15:20Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Differential gene expression analysis reveals functional roles of cryptic orange-pigmented organ in the colorless head kidney of Antarctic icefishes"]}]}],"canonical_facts":{"dc:contributor":["Cheng, Chi-Hing C.","DeVries, Arthur","Catchen, Julian"],"dc:creator":["Yoon, Eun Hye"],"dc:date":["2019-08-23T20:36:07Z","2021-08-24T09:15:20Z","2019-04-24","2019-05"],"dc:description":["We discovered an orange-pigmented, discrete body several millimeters in size embedded within the anterior end of the colorless head kidney of the hemoglobinless Antarctic icefishes. No macroscopically visible discrete organ has previously been reported in Antarctic fishes or teleost fishes in general. To assess the tissue type and functions of this cryptic orange body, we performed RNA sequencing and analyzed the orange body transcriptome relative to the surrounding head kidney tissue. We found 5,263 differentially expressed genes in the orange body, of which 4,624 genes were up-regulated and 639 down-regulated relative to the head kidney tissue. Differential gene expression analyses detected the enrichment of two main biological pathways: steroid hormone production and proline biosynthesis. The steroidogenic activities of the orange body indicate it performs functional roles of the interrenal tissue cells – the teleost homolog of the tetrapod adrenal cortex but are normally dispersed among the teleost head kidney tissue. Transcription of many cytochrome P450 enzymes participating in steroidogenesis were upregulated, potentially leading to greater levels of ROS and peroxidation of the lipid-rich steroid producing orange body cells. Proline is known to act as an antioxidant, thus, the detected enrichment in proline synthesis may be related to antioxidation defense mechanism, conferring protection against lipid peroxidation in steroid rich orange body cells.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2021-05-01","The student, Eun Hye Yoon, accepted the attached license on 2019-04-23 at 23:17.","The student, Eun Hye Yoon, submitted this Thesis for approval on 2019-04-23 at 23:34.","This Thesis was approved for publication on 2019-04-24 at 13:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13850 on 2019-08-22 at 15:07:53","Made available in DSpace on 2019-08-23T20:36:07Z (GMT). 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