{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/102933"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/102933","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Application of self-rolled-up membrane technology for enhanced neuron guidance and alignment","abstract":"Neural circuits are fundamental components that regulate critical autonomic functions throughout the human body. Disruption of neural circuits, through neurodegenerative diseases such as Parkinson’s disease or multiple sclerosis, as well as sensory neuropathies, and traumatic injuries can be incredibly debilitating. Neurological deficits have been identified as the leading cause contributing to disability adjusted life years in the United States, meaning they lead to more years of healthy life lost to disease state, leading cardiovascular diseases and cancer. Neural circuits cannot repair themselves in the same way a superficial cut, or even injury to the liver can be repaired. The inability of neural circuits to recover from injury results from a combination of the loss of essential cues that disappear post-developmentally, and complications from inflammatory response and glial scarring. Engineered therapies for neural regeneration are a burgeoning area of interest, with many techniques being explored to improve existing scaffolds for neural repair (e.g. nerve guide conduits), and develop new methods to manipulate and enhance neurite growth. This dissertation reports the use of self-rolled-up silicon nitride (SiNx) membranes to culture neurons for future applications for neuroregenerative repair. Through initial characterization of neurite growth on the microtube platform, we demonstrate enhanced alignment of neurites along the microtube topography. Following modification of microtube geometry, we found that adjustment to microtube array pitch improves neurite alignment compared to a static pitch, and increased microtube length also confers greater instances of neurite alignment. Initial experiments adding electrical stimulation to the platform reveal increased neurite growth rate and length, and increased neurite organization along the direction of the electric field. The work presented in this dissertation lays the foundation for further adaptation of the SiNx microtubes for applications for neuroregenerative therapies.","abstract_html":"Neural circuits are fundamental components that regulate critical autonomic functions throughout the human body. Disruption of neural circuits, through neurodegenerative diseases such as Parkinson’s disease or multiple sclerosis, as well as sensory neuropathies, and traumatic injuries can be incredibly debilitating. Neurological deficits have been identified as the leading cause contributing to disability adjusted life years in the United States, meaning they lead to more years of healthy life lost to disease state, leading cardiovascular diseases and cancer. Neural circuits cannot repair themselves in the same way a superficial cut, or even injury to the liver can be repaired. The inability of neural circuits to recover from injury results from a combination of the loss of essential cues that disappear post-developmentally, and complications from inflammatory response and glial scarring. Engineered therapies for neural regeneration are a burgeoning area of interest, with many techniques being explored to improve existing scaffolds for neural repair (e.g. nerve guide conduits), and develop new methods to manipulate and enhance neurite growth. This dissertation reports the use of self-rolled-up silicon nitride (SiNx) membranes to culture neurons for future applications for neuroregenerative repair. Through initial characterization of neurite growth on the microtube platform, we demonstrate enhanced alignment of neurites along the microtube topography. Following modification of microtube geometry, we found that adjustment to microtube array pitch improves neurite alignment compared to a static pitch, and increased microtube length also confers greater instances of neurite alignment. Initial experiments adding electrical stimulation to the platform reveal increased neurite growth rate and length, and increased neurite organization along the direction of the electric field. The work presented in this dissertation lays the foundation for further adaptation of the SiNx microtubes for applications for neuroregenerative therapies.","abstract_has_math":false,"creators":["Cangellaris, Olivia Vassiliki"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Gillette, Martha U.","Li, Xiuling","Bashir, Rashid","Kong, Hyunjoon"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-02-08T18:43:49Z","date_published":"2019-02-08T18:43:49Z","updated_at":"2026-07-22T22:24:42Z","subjects":["neurons","neuronal alignment","microtubes","silicon nitride nanomembranes","self-rolled-up membranes (S-RUMs)","neural scaffold","topographical cues","electrical stimulation","electric field"],"languages":["en"],"rights":["Copyright 2018 Olivia Vassiliki Cangellaris. All rights reserved."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/102933","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gillette, Martha U.","Li, Xiuling","Bashir, Rashid","Kong, Hyunjoon"]},{"key":"dc:creator","label":"Author","values":["Cangellaris, Olivia Vassiliki"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-02-08T18:43:49Z","2021-02-09T10:15:41Z","2018-12-04","2018-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["neurons","neuronal alignment","microtubes","silicon nitride nanomembranes","self-rolled-up membranes (S-RUMs)","neural scaffold","topographical cues","electrical stimulation","electric field"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2018 Olivia Vassiliki Cangellaris. 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The inability of neural circuits to recover from injury results from a combination of the loss of essential cues that disappear post-developmentally, and complications from inflammatory response and glial scarring. Engineered therapies for neural regeneration are a burgeoning area of interest, with many techniques being explored to improve existing scaffolds for neural repair (e.g. nerve guide conduits), and develop new methods to manipulate and enhance neurite growth. This dissertation reports the use of self-rolled-up silicon nitride (SiNx) membranes to culture neurons for future applications for neuroregenerative repair. Through initial characterization of neurite growth on the microtube platform, we demonstrate enhanced alignment of neurites along the microtube topography. Following modification of microtube geometry, we found that adjustment to microtube array pitch improves neurite alignment compared to a static pitch, and increased microtube length also confers greater instances of neurite alignment. Initial experiments adding electrical stimulation to the platform reveal increased neurite growth rate and length, and increased neurite organization along the direction of the electric field. The work presented in this dissertation lays the foundation for further adaptation of the SiNx microtubes for applications for neuroregenerative therapies.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-12-01","The student, Olivia Cangellaris, accepted the attached license on 2018-12-03 at 18:27.","The student, Olivia Cangellaris, submitted this Dissertation for approval on 2018-12-03 at 18:59.","This Dissertation was approved for publication on 2018-12-04 at 16:35.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13185 on 2019-02-08 at 11:40:52","Made available in DSpace on 2019-02-08T18:43:49Z (GMT). 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Engineered therapies for neural regeneration are a burgeoning area of interest, with many techniques being explored to improve existing scaffolds for neural repair (e.g. nerve guide conduits), and develop new methods to manipulate and enhance neurite growth. This dissertation reports the use of self-rolled-up silicon nitride (SiNx) membranes to culture neurons for future applications for neuroregenerative repair. Through initial characterization of neurite growth on the microtube platform, we demonstrate enhanced alignment of neurites along the microtube topography. Following modification of microtube geometry, we found that adjustment to microtube array pitch improves neurite alignment compared to a static pitch, and increased microtube length also confers greater instances of neurite alignment. Initial experiments adding electrical stimulation to the platform reveal increased neurite growth rate and length, and increased neurite organization along the direction of the electric field. The work presented in this dissertation lays the foundation for further adaptation of the SiNx microtubes for applications for neuroregenerative therapies.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-12-01","The student, Olivia Cangellaris, accepted the attached license on 2018-12-03 at 18:27.","The student, Olivia Cangellaris, submitted this Dissertation for approval on 2018-12-03 at 18:59.","This Dissertation was approved for publication on 2018-12-04 at 16:35.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13185 on 2019-02-08 at 11:40:52","Made available in DSpace on 2019-02-08T18:43:49Z (GMT). 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