{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/102833"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/102833","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characteristics of sperm binding to the mouse oviduct","abstract":"A wide variety of animals store sperm between mating and ovulation. The sperm reservoir prolongs sperm lifespan and keeps sperm in a quiescent stage due to delayed capacitation and suppressed motility. The objective of this study was to determine different characteristics of sperm binding to the oviduct. The study was conducted using the translucent mouse oviduct ex vivo. The specificity of sperm binding was determined using the isthmus, ampulla, and trachea (n = 4). The results showed no difference in binding between isthmus and trachea (P > 0.05) but binding to the ampulla was less than the isthmus (P < 0.05). Capacitation status also affected sperm binding (n = 3). The number of sperm from non-capacitated samples bound to the isthmus was significantly higher than the number of bound capacitated sperm (P < 0.05). Despite increased stiffness of oviduct cilia as female age, the number of sperm bound to the isthmus of older females was higher than younger females (P < 0.05). The number of bound sperm was different throughout estrous stages (P < 0.05) with diestrus being the highest (n = 3). Species-specific binding was also examined by allowing porcine and mouse sperm to bind the mouse isthmus (n =3). A significantly higher number of mouse sperm bound to the mouse isthmus than porcine sperm (P < 0.05). In conclusion, sperm binding to the oviduct ex vivo was not completely specific to oviduct epithelial cells or age-dependent but was species-restricted, capacitation-dependent and dependent on the stage of estrous.","abstract_html":"A wide variety of animals store sperm between mating and ovulation. The sperm reservoir prolongs sperm lifespan and keeps sperm in a quiescent stage due to delayed capacitation and suppressed motility. The objective of this study was to determine different characteristics of sperm binding to the oviduct. The study was conducted using the translucent mouse oviduct ex vivo. The specificity of sperm binding was determined using the isthmus, ampulla, and trachea (n = 4). The results showed no difference in binding between isthmus and trachea (P &gt; 0.05) but binding to the ampulla was less than the isthmus (P &lt; 0.05). Capacitation status also affected sperm binding (n = 3). The number of sperm from non-capacitated samples bound to the isthmus was significantly higher than the number of bound capacitated sperm (P &lt; 0.05). Despite increased stiffness of oviduct cilia as female age, the number of sperm bound to the isthmus of older females was higher than younger females (P &lt; 0.05). The number of bound sperm was different throughout estrous stages (P &lt; 0.05) with diestrus being the highest (n = 3). Species-specific binding was also examined by allowing porcine and mouse sperm to bind the mouse isthmus (n =3). A significantly higher number of mouse sperm bound to the mouse isthmus than porcine sperm (P &lt; 0.05). In conclusion, sperm binding to the oviduct ex vivo was not completely specific to oviduct epithelial cells or age-dependent but was species-restricted, capacitation-dependent and dependent on the stage of estrous.","abstract_has_math":false,"creators":["Doungkamchan, Kankanit"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Miller, David J.","Bahr, Janice M.","Knox, Robert V."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-02-07T20:44:21Z","date_published":"2019-02-07T20:44:21Z","updated_at":"2026-07-22T22:24:42Z","subjects":["Sperm, Oviduct, Reservoir"],"languages":["en"],"rights":["Copyright 2018 Kankanit Doungkamchan"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/102833","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Miller, David J.","Bahr, Janice M.","Knox, Robert V."]},{"key":"dc:creator","label":"Author","values":["Doungkamchan, Kankanit"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-02-07T20:44:21Z","2021-02-08T10:15:29Z","2018-12-06","2018-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Sperm, Oviduct, Reservoir"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2018 Kankanit Doungkamchan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/102833"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A wide variety of animals store sperm between mating and ovulation. The sperm reservoir prolongs sperm lifespan and keeps sperm in a quiescent stage due to delayed capacitation and suppressed motility. The objective of this study was to determine different characteristics of sperm binding to the oviduct. The study was conducted using the translucent mouse oviduct ex vivo. The specificity of sperm binding was determined using the isthmus, ampulla, and trachea (n = 4). The results showed no difference in binding between isthmus and trachea (P > 0.05) but binding to the ampulla was less than the isthmus (P < 0.05). Capacitation status also affected sperm binding (n = 3). The number of sperm from non-capacitated samples bound to the isthmus was significantly higher than the number of bound capacitated sperm (P < 0.05). Despite increased stiffness of oviduct cilia as female age, the number of sperm bound to the isthmus of older females was higher than younger females (P < 0.05). The number of bound sperm was different throughout estrous stages (P < 0.05) with diestrus being the highest (n = 3). Species-specific binding was also examined by allowing porcine and mouse sperm to bind the mouse isthmus (n =3). A significantly higher number of mouse sperm bound to the mouse isthmus than porcine sperm (P < 0.05). In conclusion, sperm binding to the oviduct ex vivo was not completely specific to oviduct epithelial cells or age-dependent but was species-restricted, capacitation-dependent and dependent on the stage of estrous.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-12-01","The student, Kankanit Doungkamchan, accepted the attached license on 2018-12-04 at 10:40.","The student, Kankanit Doungkamchan, submitted this Thesis for approval on 2018-12-04 at 11:07.","This Thesis was approved for publication on 2018-12-06 at 13:47.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13194 on 2019-02-07 at 14:18:59","Made available in DSpace on 2019-02-07T20:44:21Z (GMT). No. of bitstreams: 13 DOUNGKAMCHAN-THESIS-2018.pdf: 17860113 bytes, checksum: 3fd87341a51ba0ca9d50ce119c3c42c2 (MD5) Supplemental movie 1 (isthmus).avi: 108709888 bytes, checksum: c746f8fbe000b93a3ec58a62c79a95f3 (MD5) Supplemental movie 10 (pig sperm).avi: 69042688 bytes, checksum: 80b35b222cdda93258cac6db2e14be7b (MD5) Supplemental movie 11 (mouse sperm).avi: 65786368 bytes, checksum: 1b33bcb4f1a7bea49a1924919f98714f (MD5) Supplemental movie 2 (ampulla).avi: 90499072 bytes, checksum: 5e13b1f0592fe53c6c6e64416d4ebb04 (MD5) Supplemental movie 3 (trachea).avi: 169226752 bytes, checksum: 14712f7151579c8832d2582256bd5057 (MD5) Supplemental movie 4 (non-capacitated sperm).avi: 118823424 bytes, checksum: 69b99c36e73800ca433b063d76d2dc0d (MD5) Supplemental movie 5 (capacitated sperm).avi: 173675520 bytes, checksum: 7402a09bfa60e1b40c812cf2e29704d2 (MD5) Supplemental movie 6 (non-viscous medium).avi: 133476864 bytes, checksum: 1bb6d2b9cf84cfa5942dfe2e6489dae3 (MD5) Supplemental movie 7 (viscous medium).avi: 45001216 bytes, checksum: 6458e77aadf39fb8629ebbc6b03a44a1 (MD5) Supplemental movie 8 (87 weeks old)).avi: 61674496 bytes, checksum: 17b3a42a86fa531cd38b3fd23d493cc3 (MD5) Supplemental movie 9 (13 weeks old).avi: 55770112 bytes, checksum: d499682aef329e1cd00659873624b796 (MD5) LICENSE.txt: 4218 bytes, checksum: 6a7df31b793dbef99c7b94087c6f18a5 (MD5) Previous issue date: 2018-12-06","Embargo set by: Seth Robbins for item 109859 Lift date: 2021-02-07T20:44:35Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 109859 on 2021-02-08T10:15:29Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Characteristics of sperm binding to the mouse oviduct"]}]}],"canonical_facts":{"dc:contributor":["Miller, David J.","Bahr, Janice M.","Knox, Robert V."],"dc:creator":["Doungkamchan, Kankanit"],"dc:date":["2019-02-07T20:44:21Z","2021-02-08T10:15:29Z","2018-12-06","2018-12"],"dc:description":["A wide variety of animals store sperm between mating and ovulation. The sperm reservoir prolongs sperm lifespan and keeps sperm in a quiescent stage due to delayed capacitation and suppressed motility. The objective of this study was to determine different characteristics of sperm binding to the oviduct. The study was conducted using the translucent mouse oviduct ex vivo. The specificity of sperm binding was determined using the isthmus, ampulla, and trachea (n = 4). The results showed no difference in binding between isthmus and trachea (P > 0.05) but binding to the ampulla was less than the isthmus (P < 0.05). Capacitation status also affected sperm binding (n = 3). The number of sperm from non-capacitated samples bound to the isthmus was significantly higher than the number of bound capacitated sperm (P < 0.05). Despite increased stiffness of oviduct cilia as female age, the number of sperm bound to the isthmus of older females was higher than younger females (P < 0.05). The number of bound sperm was different throughout estrous stages (P < 0.05) with diestrus being the highest (n = 3). Species-specific binding was also examined by allowing porcine and mouse sperm to bind the mouse isthmus (n =3). A significantly higher number of mouse sperm bound to the mouse isthmus than porcine sperm (P < 0.05). In conclusion, sperm binding to the oviduct ex vivo was not completely specific to oviduct epithelial cells or age-dependent but was species-restricted, capacitation-dependent and dependent on the stage of estrous.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-12-01","The student, Kankanit Doungkamchan, accepted the attached license on 2018-12-04 at 10:40.","The student, Kankanit Doungkamchan, submitted this Thesis for approval on 2018-12-04 at 11:07.","This Thesis was approved for publication on 2018-12-06 at 13:47.","DSpace SAF Submission Ingestion Package generated from Vireo submission #13194 on 2019-02-07 at 14:18:59","Made available in DSpace on 2019-02-07T20:44:21Z (GMT). No. of bitstreams: 13 DOUNGKAMCHAN-THESIS-2018.pdf: 17860113 bytes, checksum: 3fd87341a51ba0ca9d50ce119c3c42c2 (MD5) Supplemental movie 1 (isthmus).avi: 108709888 bytes, checksum: c746f8fbe000b93a3ec58a62c79a95f3 (MD5) Supplemental movie 10 (pig sperm).avi: 69042688 bytes, checksum: 80b35b222cdda93258cac6db2e14be7b (MD5) Supplemental movie 11 (mouse sperm).avi: 65786368 bytes, checksum: 1b33bcb4f1a7bea49a1924919f98714f (MD5) Supplemental movie 2 (ampulla).avi: 90499072 bytes, checksum: 5e13b1f0592fe53c6c6e64416d4ebb04 (MD5) Supplemental movie 3 (trachea).avi: 169226752 bytes, checksum: 14712f7151579c8832d2582256bd5057 (MD5) Supplemental movie 4 (non-capacitated sperm).avi: 118823424 bytes, checksum: 69b99c36e73800ca433b063d76d2dc0d (MD5) Supplemental movie 5 (capacitated sperm).avi: 173675520 bytes, checksum: 7402a09bfa60e1b40c812cf2e29704d2 (MD5) Supplemental movie 6 (non-viscous medium).avi: 133476864 bytes, checksum: 1bb6d2b9cf84cfa5942dfe2e6489dae3 (MD5) Supplemental movie 7 (viscous medium).avi: 45001216 bytes, checksum: 6458e77aadf39fb8629ebbc6b03a44a1 (MD5) Supplemental movie 8 (87 weeks old)).avi: 61674496 bytes, checksum: 17b3a42a86fa531cd38b3fd23d493cc3 (MD5) Supplemental movie 9 (13 weeks old).avi: 55770112 bytes, checksum: d499682aef329e1cd00659873624b796 (MD5) LICENSE.txt: 4218 bytes, checksum: 6a7df31b793dbef99c7b94087c6f18a5 (MD5) Previous issue date: 2018-12-06","Embargo set by: Seth Robbins for item 109859 Lift date: 2021-02-07T20:44:35Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 109859 on 2021-02-08T10:15:29Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/102833"],"dc:language":["en"],"dc:rights":["Copyright 2018 Kankanit Doungkamchan"],"dc:subject":["Sperm, Oviduct, Reservoir"],"dc:title":["Characteristics of sperm binding to the mouse oviduct"],"dc:type":["text"],"thesis:degree_discipline":["Animal Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:42Z"}