{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/101730"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/101730","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Epigenetic regulation of genes related to lipid metabolism by microrna in mice fed high fat diet","abstract":"High fat diet impacts lipid metabolism by altering the transportation, oxidation, and storage of fatty acids. Lipoprotein lipase (LPL) plays a critical role in lipid metabolism by catalyzing triglyceride hydrolysis and lipoprotein uptake in multiple tissues. A previous study reported that miR-29b negatively regulated LPL expression in mammary epithelial cells transfected with miR-29b mimics. The present study investigated changes in LPL expression and epigenetic mechanisms by the miR-29 family in different tissues in mice fed a high fat diet. Five-week old male CBA mice were fed with either a control diet (Con group, 10% kcal from fat) or a high fat diet (HF group, 45% kcal from fat) ad libitum for 11 weeks. The results showed that LPL mRNA was increased in adipose, muscle and colon in response to high fat diet. However, LPL mRNA expression decreased in the liver by high fat diet as well as hepatic lipase (HL). The results also showed the highest expression level of LPL mRNA in adipose tissue, followed by muscle, colon, and liver. Meanwhile, high fat diet reduced the expression of miR-29a/b, predicted suppressors of LPL from miR-29 family, in adipose tissue. Genomic analysis predicted several potential transcription factors of miR-29 family members that suppress the expression of miR-29s. At mRNA level, some of these transcription factors, c-Myc and EZH2, were significantly activated in response to HF diet. The present results indicated that the LPL expression could be activated by high fat diet in multiple tissues and the induction of LPL is post-transcriptionally regulated by miR-29a/b. Furthermore, the transcription of miR-29 in mice adipose was regulated by certain transcriptional factors. Overall, LPL mRNA altered in multiple tissues in response to high fat diet and is potentially regulated through transcription factors and microRNAs.","abstract_html":"High fat diet impacts lipid metabolism by altering the transportation, oxidation, and storage of fatty acids. Lipoprotein lipase (LPL) plays a critical role in lipid metabolism by catalyzing triglyceride hydrolysis and lipoprotein uptake in multiple tissues. A previous study reported that miR-29b negatively regulated LPL expression in mammary epithelial cells transfected with miR-29b mimics. The present study investigated changes in LPL expression and epigenetic mechanisms by the miR-29 family in different tissues in mice fed a high fat diet. Five-week old male CBA mice were fed with either a control diet (Con group, 10% kcal from fat) or a high fat diet (HF group, 45% kcal from fat) ad libitum for 11 weeks. The results showed that LPL mRNA was increased in adipose, muscle and colon in response to high fat diet. However, LPL mRNA expression decreased in the liver by high fat diet as well as hepatic lipase (HL). The results also showed the highest expression level of LPL mRNA in adipose tissue, followed by muscle, colon, and liver. Meanwhile, high fat diet reduced the expression of miR-29a/b, predicted suppressors of LPL from miR-29 family, in adipose tissue. Genomic analysis predicted several potential transcription factors of miR-29 family members that suppress the expression of miR-29s. At mRNA level, some of these transcription factors, c-Myc and EZH2, were significantly activated in response to HF diet. The present results indicated that the LPL expression could be activated by high fat diet in multiple tissues and the induction of LPL is post-transcriptionally regulated by miR-29a/b. Furthermore, the transcription of miR-29 in mice adipose was regulated by certain transcriptional factors. Overall, LPL mRNA altered in multiple tissues in response to high fat diet and is potentially regulated through transcription factors and microRNAs.","abstract_has_math":false,"creators":["Tang, Xinyu"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Chen, Hong"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-09-27T16:34:23Z","date_published":"2018-09-27T16:34:23Z","updated_at":"2026-07-22T22:24:40Z","subjects":["Lipid metabolism","MicroRNAs","Transcriptional factor","Promoter region"],"languages":["en"],"rights":["Copyright 2018 Xinyu Tang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/101730","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Chen, Hong"]},{"key":"dc:creator","label":"Author","values":["Tang, Xinyu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-09-27T16:34:23Z","2020-09-28T09:15:24Z","2018-07-19","2018-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Lipid metabolism","MicroRNAs","Transcriptional factor","Promoter region"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2018 Xinyu Tang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/101730"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["High fat diet impacts lipid metabolism by altering the transportation, oxidation, and storage of fatty acids. Lipoprotein lipase (LPL) plays a critical role in lipid metabolism by catalyzing triglyceride hydrolysis and lipoprotein uptake in multiple tissues. A previous study reported that miR-29b negatively regulated LPL expression in mammary epithelial cells transfected with miR-29b mimics. The present study investigated changes in LPL expression and epigenetic mechanisms by the miR-29 family in different tissues in mice fed a high fat diet. Five-week old male CBA mice were fed with either a control diet (Con group, 10% kcal from fat) or a high fat diet (HF group, 45% kcal from fat) ad libitum for 11 weeks. The results showed that LPL mRNA was increased in adipose, muscle and colon in response to high fat diet. However, LPL mRNA expression decreased in the liver by high fat diet as well as hepatic lipase (HL). The results also showed the highest expression level of LPL mRNA in adipose tissue, followed by muscle, colon, and liver. Meanwhile, high fat diet reduced the expression of miR-29a/b, predicted suppressors of LPL from miR-29 family, in adipose tissue. Genomic analysis predicted several potential transcription factors of miR-29 family members that suppress the expression of miR-29s. At mRNA level, some of these transcription factors, c-Myc and EZH2, were significantly activated in response to HF diet. The present results indicated that the LPL expression could be activated by high fat diet in multiple tissues and the induction of LPL is post-transcriptionally regulated by miR-29a/b. Furthermore, the transcription of miR-29 in mice adipose was regulated by certain transcriptional factors. Overall, LPL mRNA altered in multiple tissues in response to high fat diet and is potentially regulated through transcription factors and microRNAs.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-08-01","The student, Xinyu Tang, accepted the attached license on 2018-07-19 at 11:58.","The student, Xinyu Tang, submitted this Thesis for approval on 2018-07-19 at 11:59.","This Thesis was approved for publication on 2018-07-19 at 16:22.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12941 on 2018-09-27 at 11:19:34","Made available in DSpace on 2018-09-27T16:34:23Z (GMT). No. of bitstreams: 2 TANG-THESIS-2018.pdf: 553243 bytes, checksum: 0088f1f14b5e080f296191212188c4b2 (MD5) LICENSE.txt: 4207 bytes, checksum: 6698dd5ff0c704f4cc2df8444ad36a0b (MD5) Previous issue date: 2018-07-19","Embargo set by: Seth Robbins for item 107830 Lift date: 2020-09-27T16:34:29Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 107830 on 2020-09-28T09:15:24Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Epigenetic regulation of genes related to lipid metabolism by microrna in mice fed high fat diet"]}]}],"canonical_facts":{"dc:contributor":["Chen, Hong"],"dc:creator":["Tang, Xinyu"],"dc:date":["2018-09-27T16:34:23Z","2020-09-28T09:15:24Z","2018-07-19","2018-08"],"dc:description":["High fat diet impacts lipid metabolism by altering the transportation, oxidation, and storage of fatty acids. Lipoprotein lipase (LPL) plays a critical role in lipid metabolism by catalyzing triglyceride hydrolysis and lipoprotein uptake in multiple tissues. A previous study reported that miR-29b negatively regulated LPL expression in mammary epithelial cells transfected with miR-29b mimics. The present study investigated changes in LPL expression and epigenetic mechanisms by the miR-29 family in different tissues in mice fed a high fat diet. Five-week old male CBA mice were fed with either a control diet (Con group, 10% kcal from fat) or a high fat diet (HF group, 45% kcal from fat) ad libitum for 11 weeks. The results showed that LPL mRNA was increased in adipose, muscle and colon in response to high fat diet. However, LPL mRNA expression decreased in the liver by high fat diet as well as hepatic lipase (HL). The results also showed the highest expression level of LPL mRNA in adipose tissue, followed by muscle, colon, and liver. Meanwhile, high fat diet reduced the expression of miR-29a/b, predicted suppressors of LPL from miR-29 family, in adipose tissue. Genomic analysis predicted several potential transcription factors of miR-29 family members that suppress the expression of miR-29s. At mRNA level, some of these transcription factors, c-Myc and EZH2, were significantly activated in response to HF diet. The present results indicated that the LPL expression could be activated by high fat diet in multiple tissues and the induction of LPL is post-transcriptionally regulated by miR-29a/b. Furthermore, the transcription of miR-29 in mice adipose was regulated by certain transcriptional factors. Overall, LPL mRNA altered in multiple tissues in response to high fat diet and is potentially regulated through transcription factors and microRNAs.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-08-01","The student, Xinyu Tang, accepted the attached license on 2018-07-19 at 11:58.","The student, Xinyu Tang, submitted this Thesis for approval on 2018-07-19 at 11:59.","This Thesis was approved for publication on 2018-07-19 at 16:22.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12941 on 2018-09-27 at 11:19:34","Made available in DSpace on 2018-09-27T16:34:23Z (GMT). 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