{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/101509"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/101509","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Cryopreservation of tissue engineered skeletal muscle","abstract":"Tissue engineered skeletal muscle plays an important role not only in the field of regenerative medicine, but also in emerging areas such as soft robotics, organ-on-a-chip models of disease, and drug testing. However, further expansion of the applications of tissue engineered skeletal muscle models will require a suitable method for their long-term storage and shipment. Cryopreservation has long been the standard for long-term cell storage, but when it comes to the freezing of 3D tissues, many complications arise due to heat and mass transfer limitations. Here, we use a tissue engineered skeletal muscle bioactuator as a model to characterize the effects of freezing on skeletal muscle viability, gene expression, myotube structure, and force generation. We optimize the freezing medium composition and compare the effects of freezing on both undifferentiated and differentiated engineered skeletal muscle tissue constructs. We report an optimized protocol of freezing skeletal muscle constructs while undifferentiated, which not only maintains cell viability, but leads to a 3-fold increase in force production as compared to unfrozen muscle. The reported timeline for skeletal muscle tissue fabrication, freezing, and revival not only promotes a more streamlined fabrication process, but will further enable collaborative research efforts through the shipment of pre-formed skeletal muscle constructs.","abstract_html":"Tissue engineered skeletal muscle plays an important role not only in the field of regenerative medicine, but also in emerging areas such as soft robotics, organ-on-a-chip models of disease, and drug testing. However, further expansion of the applications of tissue engineered skeletal muscle models will require a suitable method for their long-term storage and shipment. Cryopreservation has long been the standard for long-term cell storage, but when it comes to the freezing of 3D tissues, many complications arise due to heat and mass transfer limitations. Here, we use a tissue engineered skeletal muscle bioactuator as a model to characterize the effects of freezing on skeletal muscle viability, gene expression, myotube structure, and force generation. We optimize the freezing medium composition and compare the effects of freezing on both undifferentiated and differentiated engineered skeletal muscle tissue constructs. We report an optimized protocol of freezing skeletal muscle constructs while undifferentiated, which not only maintains cell viability, but leads to a 3-fold increase in force production as compared to unfrozen muscle. The reported timeline for skeletal muscle tissue fabrication, freezing, and revival not only promotes a more streamlined fabrication process, but will further enable collaborative research efforts through the shipment of pre-formed skeletal muscle constructs.","abstract_has_math":false,"creators":["Grant, Lauren"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Bashir, Rashid"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-09-27T16:17:33Z","date_published":"2018-09-27T16:17:33Z","updated_at":"2026-07-22T22:24:40Z","subjects":["biobot","bioactuator","skeletal muscle","tissue engineering","cryopreservation"],"languages":["en"],"rights":["Copyright 2018 Lauren Grant"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/101509","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bashir, Rashid"]},{"key":"dc:creator","label":"Author","values":["Grant, Lauren"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-09-27T16:17:33Z","2018-07-02","2018-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["biobot","bioactuator","skeletal muscle","tissue engineering","cryopreservation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2018 Lauren Grant"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/101509"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Tissue engineered skeletal muscle plays an important role not only in the field of regenerative medicine, but also in emerging areas such as soft robotics, organ-on-a-chip models of disease, and drug testing. However, further expansion of the applications of tissue engineered skeletal muscle models will require a suitable method for their long-term storage and shipment. Cryopreservation has long been the standard for long-term cell storage, but when it comes to the freezing of 3D tissues, many complications arise due to heat and mass transfer limitations. Here, we use a tissue engineered skeletal muscle bioactuator as a model to characterize the effects of freezing on skeletal muscle viability, gene expression, myotube structure, and force generation. We optimize the freezing medium composition and compare the effects of freezing on both undifferentiated and differentiated engineered skeletal muscle tissue constructs. We report an optimized protocol of freezing skeletal muscle constructs while undifferentiated, which not only maintains cell viability, but leads to a 3-fold increase in force production as compared to unfrozen muscle. The reported timeline for skeletal muscle tissue fabrication, freezing, and revival not only promotes a more streamlined fabrication process, but will further enable collaborative research efforts through the shipment of pre-formed skeletal muscle constructs.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2018-09-27 without embargo terms","The student, Lauren Grant, accepted the attached license on 2018-06-27 at 16:29.","The student, Lauren Grant, submitted this Thesis for approval on 2018-06-27 at 16:51.","This Thesis was approved for publication on 2018-07-02 at 09:52.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12679 on 2018-09-27 at 10:45:57","Made available in DSpace on 2018-09-27T16:17:33Z (GMT). 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However, further expansion of the applications of tissue engineered skeletal muscle models will require a suitable method for their long-term storage and shipment. Cryopreservation has long been the standard for long-term cell storage, but when it comes to the freezing of 3D tissues, many complications arise due to heat and mass transfer limitations. Here, we use a tissue engineered skeletal muscle bioactuator as a model to characterize the effects of freezing on skeletal muscle viability, gene expression, myotube structure, and force generation. We optimize the freezing medium composition and compare the effects of freezing on both undifferentiated and differentiated engineered skeletal muscle tissue constructs. We report an optimized protocol of freezing skeletal muscle constructs while undifferentiated, which not only maintains cell viability, but leads to a 3-fold increase in force production as compared to unfrozen muscle. The reported timeline for skeletal muscle tissue fabrication, freezing, and revival not only promotes a more streamlined fabrication process, but will further enable collaborative research efforts through the shipment of pre-formed skeletal muscle constructs.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2018-09-27 without embargo terms","The student, Lauren Grant, accepted the attached license on 2018-06-27 at 16:29.","The student, Lauren Grant, submitted this Thesis for approval on 2018-06-27 at 16:51.","This Thesis was approved for publication on 2018-07-02 at 09:52.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12679 on 2018-09-27 at 10:45:57","Made available in DSpace on 2018-09-27T16:17:33Z (GMT). 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