{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/101363"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/101363","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Inverted micelles: a novel method of theranostic agent encapsulation for enhanced nano-delivery","abstract":"This thesis outlines an innovative solution for packaging an expansive set of theranostic agents for high-throughput intracellular delivery, which offers numerous advantages over liposomes and other contemporary encapsulation modalities. This encapsulation method works by using a simple 2-step phase-transfer chemistry. The first step consists of enveloping the agents within an inverted micelle capsule, and in the second step, they are repackaged with an outer lipid-based shell to produce a structure termed the NanoCapsule (NCAP). Our encapsulation procedure is adaptable and robust, with modifiable size, chemistry, and surface properties and it is compatible with agents such as ions, small molecules, and ~10 nm nanoparticles. This method greatly simplifies hydrophobic encapsulation and surface modification, which is otherwise a costly and technically challenging procedure that relies on anaerobic reactions in an organic medium. Our encapsulation procedure can also be scaled with high-throughput efficacy. Furthermore, the inverted micelles and NCAP coating consist of materials that have been shown to provide excellent biocompatibility. With this methodology, agents are encapsulated without a significant amount of aqueous solvent in the lumen of the nanoparticle, thus, delivering a more concentrated and stable payload to the tumor site without the leaky tendencies of liposomal encapsulation. Within this thesis, we show how the NCAP architecture retains long-term stability and quantifiably enhances payload delivery intracellularly through in vitro and in vivo investigations. Furthermore, the highly customizable nature of our encapsulation methodology is especially desirable for theranostic applications in personalized medicine. Therefore, our strategy could become a widely-used methodology for encapsulating and delivering personalized theranostics, customized to match precise therapeutic needs.","abstract_html":"This thesis outlines an innovative solution for packaging an expansive set of theranostic agents for high-throughput intracellular delivery, which offers numerous advantages over liposomes and other contemporary encapsulation modalities. This encapsulation method works by using a simple 2-step phase-transfer chemistry. The first step consists of enveloping the agents within an inverted micelle capsule, and in the second step, they are repackaged with an outer lipid-based shell to produce a structure termed the NanoCapsule (NCAP). Our encapsulation procedure is adaptable and robust, with modifiable size, chemistry, and surface properties and it is compatible with agents such as ions, small molecules, and ~10 nm nanoparticles. This method greatly simplifies hydrophobic encapsulation and surface modification, which is otherwise a costly and technically challenging procedure that relies on anaerobic reactions in an organic medium. Our encapsulation procedure can also be scaled with high-throughput efficacy. Furthermore, the inverted micelles and NCAP coating consist of materials that have been shown to provide excellent biocompatibility. With this methodology, agents are encapsulated without a significant amount of aqueous solvent in the lumen of the nanoparticle, thus, delivering a more concentrated and stable payload to the tumor site without the leaky tendencies of liposomal encapsulation. Within this thesis, we show how the NCAP architecture retains long-term stability and quantifiably enhances payload delivery intracellularly through in vitro and in vivo investigations. Furthermore, the highly customizable nature of our encapsulation methodology is especially desirable for theranostic applications in personalized medicine. Therefore, our strategy could become a widely-used methodology for encapsulating and delivering personalized theranostics, customized to match precise therapeutic needs.","abstract_has_math":false,"creators":["Daza, Enrique Alejandro"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Pan, Dipanjan","Irudayaraj, Joseph","Dobrucki, Wawrzyniec","Underhill, Gregory H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-09-04T20:47:28Z","date_published":"2018-09-04T20:47:28Z","updated_at":"2026-07-22T22:24:38Z","subjects":["Nanomedicine","Nanoparticles","Inverted Micelles","Theranostics","Drug Delivery","Nano-Delivery"],"languages":["en"],"rights":["Copyright 2018 Enrique A Daza"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/101363","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pan, Dipanjan","Irudayaraj, Joseph","Dobrucki, Wawrzyniec","Underhill, Gregory H."]},{"key":"dc:creator","label":"Author","values":["Daza, Enrique Alejandro"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-09-04T20:47:28Z","2020-09-05T09:15:16Z","2018-04-20","2018-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nanomedicine","Nanoparticles","Inverted Micelles","Theranostics","Drug Delivery","Nano-Delivery"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2018 Enrique A Daza"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/101363"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This thesis outlines an innovative solution for packaging an expansive set of theranostic agents for high-throughput intracellular delivery, which offers numerous advantages over liposomes and other contemporary encapsulation modalities. This encapsulation method works by using a simple 2-step phase-transfer chemistry. The first step consists of enveloping the agents within an inverted micelle capsule, and in the second step, they are repackaged with an outer lipid-based shell to produce a structure termed the NanoCapsule (NCAP). Our encapsulation procedure is adaptable and robust, with modifiable size, chemistry, and surface properties and it is compatible with agents such as ions, small molecules, and ~10 nm nanoparticles. This method greatly simplifies hydrophobic encapsulation and surface modification, which is otherwise a costly and technically challenging procedure that relies on anaerobic reactions in an organic medium. Our encapsulation procedure can also be scaled with high-throughput efficacy. Furthermore, the inverted micelles and NCAP coating consist of materials that have been shown to provide excellent biocompatibility. With this methodology, agents are encapsulated without a significant amount of aqueous solvent in the lumen of the nanoparticle, thus, delivering a more concentrated and stable payload to the tumor site without the leaky tendencies of liposomal encapsulation. Within this thesis, we show how the NCAP architecture retains long-term stability and quantifiably enhances payload delivery intracellularly through in vitro and in vivo investigations. Furthermore, the highly customizable nature of our encapsulation methodology is especially desirable for theranostic applications in personalized medicine. Therefore, our strategy could become a widely-used methodology for encapsulating and delivering personalized theranostics, customized to match precise therapeutic needs.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-05-01","The student, Enrique Daza, accepted the attached license on 2018-04-20 at 14:11.","The student, Enrique Daza, submitted this Dissertation for approval on 2018-04-20 at 14:18.","This Dissertation was approved for publication on 2018-04-20 at 15:38.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12407 on 2018-08-31 at 17:30:13","Made available in DSpace on 2018-09-04T20:47:28Z (GMT). No. of bitstreams: 2 DAZA-DISSERTATION-2018.pdf: 6586516 bytes, checksum: a2ba13a28e39625eaf3730b9ff50ba6e (MD5) LICENSE.txt: 4209 bytes, checksum: ee08bde0a5f4c60bca6a2fe25f8ee11c (MD5) Previous issue date: 2018-04-20","Embargo set by: Seth Robbins for item 107448 Lift date: 2020-09-04T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 107448 Lift date: 2020-09-04T20:50:11Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 107448 on 2020-09-05T09:15:16Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Inverted micelles: a novel method of theranostic agent encapsulation for enhanced nano-delivery"]}]}],"canonical_facts":{"dc:contributor":["Pan, Dipanjan","Irudayaraj, Joseph","Dobrucki, Wawrzyniec","Underhill, Gregory H."],"dc:creator":["Daza, Enrique Alejandro"],"dc:date":["2018-09-04T20:47:28Z","2020-09-05T09:15:16Z","2018-04-20","2018-05"],"dc:description":["This thesis outlines an innovative solution for packaging an expansive set of theranostic agents for high-throughput intracellular delivery, which offers numerous advantages over liposomes and other contemporary encapsulation modalities. This encapsulation method works by using a simple 2-step phase-transfer chemistry. The first step consists of enveloping the agents within an inverted micelle capsule, and in the second step, they are repackaged with an outer lipid-based shell to produce a structure termed the NanoCapsule (NCAP). Our encapsulation procedure is adaptable and robust, with modifiable size, chemistry, and surface properties and it is compatible with agents such as ions, small molecules, and ~10 nm nanoparticles. This method greatly simplifies hydrophobic encapsulation and surface modification, which is otherwise a costly and technically challenging procedure that relies on anaerobic reactions in an organic medium. Our encapsulation procedure can also be scaled with high-throughput efficacy. Furthermore, the inverted micelles and NCAP coating consist of materials that have been shown to provide excellent biocompatibility. With this methodology, agents are encapsulated without a significant amount of aqueous solvent in the lumen of the nanoparticle, thus, delivering a more concentrated and stable payload to the tumor site without the leaky tendencies of liposomal encapsulation. Within this thesis, we show how the NCAP architecture retains long-term stability and quantifiably enhances payload delivery intracellularly through in vitro and in vivo investigations. Furthermore, the highly customizable nature of our encapsulation methodology is especially desirable for theranostic applications in personalized medicine. Therefore, our strategy could become a widely-used methodology for encapsulating and delivering personalized theranostics, customized to match precise therapeutic needs.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-05-01","The student, Enrique Daza, accepted the attached license on 2018-04-20 at 14:11.","The student, Enrique Daza, submitted this Dissertation for approval on 2018-04-20 at 14:18.","This Dissertation was approved for publication on 2018-04-20 at 15:38.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12407 on 2018-08-31 at 17:30:13","Made available in DSpace on 2018-09-04T20:47:28Z (GMT). No. of bitstreams: 2 DAZA-DISSERTATION-2018.pdf: 6586516 bytes, checksum: a2ba13a28e39625eaf3730b9ff50ba6e (MD5) LICENSE.txt: 4209 bytes, checksum: ee08bde0a5f4c60bca6a2fe25f8ee11c (MD5) Previous issue date: 2018-04-20","Embargo set by: Seth Robbins for item 107448 Lift date: 2020-09-04T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 107448 Lift date: 2020-09-04T20:50:11Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 107448 on 2020-09-05T09:15:16Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/101363"],"dc:language":["en"],"dc:rights":["Copyright 2018 Enrique A Daza"],"dc:subject":["Nanomedicine","Nanoparticles","Inverted Micelles","Theranostics","Drug Delivery","Nano-Delivery"],"dc:title":["Inverted micelles: a novel method of theranostic agent encapsulation for enhanced nano-delivery"],"dc:type":["text"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:38Z"}