{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/101360"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/101360","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Long-term n fertilization affects soil bacterial communities in agronomic fields","abstract":"Soil microbial communities influence plant productivity, chemistry and structure of soil and nutrient cycling. In turn, long-term nitrogen fertilization changes soil physical and chemical properties and thus influences soil microbiomes. In-depth understanding of the long-term nitrogen fertilization effects on soil microbial communities is critical for improving fertilization practices to optimize the microbiomes for soil health, productivity and sustainability. Our goal was to characterize the microbial community structure under long-term N gradient treatment in continuous corn (CCC) and to investigate the response of different soil microbial groups involved in the N cycle to different N fertilization levels. Field trial in CCC on three N fertilization rates (0, 210, and 280 kg N/ha) is arranged in a randomized complete block design (RCBD) with three replications. Using 16S rRNA gene-based pyrosequencing analysis of the V4 region and DNA extracted from soil in a 33-year-old agricultural field trial, we characterized the structure, composition and nitrogen cycling function of the bacterial communities involved. The downstream bioinformatics processing and analysis were conducted with QIIME (Quantitative Insights Into Microbial Ecology). V4 region of 16S rDNA gene sequences were clustered into operational taxonomic units (OTUs) and the bacterial community composition and diversity were analyzed based on the OTUs extracted. We applied principal component analyses (PCA) and canonical discriminant analysis (CDA) to our major bacterial phyla dataset. Our results indicate that high N fertilization level tended to decrease the diversity and richness of soil bacterial community and altered the relative abundance of the major bacterial phyla. There was a significant response of different soil bacterial groups involved in the N cycle to different N fertilization levels.","abstract_html":"Soil microbial communities influence plant productivity, chemistry and structure of soil and nutrient cycling. In turn, long-term nitrogen fertilization changes soil physical and chemical properties and thus influences soil microbiomes. In-depth understanding of the long-term nitrogen fertilization effects on soil microbial communities is critical for improving fertilization practices to optimize the microbiomes for soil health, productivity and sustainability. Our goal was to characterize the microbial community structure under long-term N gradient treatment in continuous corn (CCC) and to investigate the response of different soil microbial groups involved in the N cycle to different N fertilization levels. Field trial in CCC on three N fertilization rates (0, 210, and 280 kg N/ha) is arranged in a randomized complete block design (RCBD) with three replications. Using 16S rRNA gene-based pyrosequencing analysis of the V4 region and DNA extracted from soil in a 33-year-old agricultural field trial, we characterized the structure, composition and nitrogen cycling function of the bacterial communities involved. The downstream bioinformatics processing and analysis were conducted with QIIME (Quantitative Insights Into Microbial Ecology). V4 region of 16S rDNA gene sequences were clustered into operational taxonomic units (OTUs) and the bacterial community composition and diversity were analyzed based on the OTUs extracted. We applied principal component analyses (PCA) and canonical discriminant analysis (CDA) to our major bacterial phyla dataset. Our results indicate that high N fertilization level tended to decrease the diversity and richness of soil bacterial community and altered the relative abundance of the major bacterial phyla. There was a significant response of different soil bacterial groups involved in the N cycle to different N fertilization levels.","abstract_has_math":false,"creators":["Sun, Renpeng"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioinformatics","degree_department":null,"school":null,"contributors":["Villamil, Maria Bonita"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-04-23","date_published":"2018-04-23","updated_at":"2026-07-22T22:24:38Z","subjects":["long-term N fertilization","soil bacterial communities"],"languages":["en"],"rights":["Copyright 2018 Renpeng Sun"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/101360","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Villamil, Maria Bonita"]},{"key":"dc:creator","label":"Author","values":["Sun, Renpeng"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-04-23","2018-09-04T20:47:27Z","2020-09-05T09:15:20Z","2018-05"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioinformatics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["long-term N fertilization","soil bacterial communities"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2018 Renpeng Sun"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/101360"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Soil microbial communities influence plant productivity, chemistry and structure of soil and nutrient cycling. In turn, long-term nitrogen fertilization changes soil physical and chemical properties and thus influences soil microbiomes. In-depth understanding of the long-term nitrogen fertilization effects on soil microbial communities is critical for improving fertilization practices to optimize the microbiomes for soil health, productivity and sustainability. Our goal was to characterize the microbial community structure under long-term N gradient treatment in continuous corn (CCC) and to investigate the response of different soil microbial groups involved in the N cycle to different N fertilization levels. Field trial in CCC on three N fertilization rates (0, 210, and 280 kg N/ha) is arranged in a randomized complete block design (RCBD) with three replications. Using 16S rRNA gene-based pyrosequencing analysis of the V4 region and DNA extracted from soil in a 33-year-old agricultural field trial, we characterized the structure, composition and nitrogen cycling function of the bacterial communities involved. The downstream bioinformatics processing and analysis were conducted with QIIME (Quantitative Insights Into Microbial Ecology). V4 region of 16S rDNA gene sequences were clustered into operational taxonomic units (OTUs) and the bacterial community composition and diversity were analyzed based on the OTUs extracted. We applied principal component analyses (PCA) and canonical discriminant analysis (CDA) to our major bacterial phyla dataset. Our results indicate that high N fertilization level tended to decrease the diversity and richness of soil bacterial community and altered the relative abundance of the major bacterial phyla. There was a significant response of different soil bacterial groups involved in the N cycle to different N fertilization levels.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-05-01","The student, Renpeng Sun, accepted the attached license on 2018-04-19 at 14:20.","The student, Renpeng Sun, submitted this Thesis for approval on 2018-04-19 at 14:25.","This Thesis was approved for publication on 2018-04-23 at 09:49.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12384 on 2018-08-31 at 17:30:11","Made available in DSpace on 2018-09-04T20:47:27Z (GMT). No. of bitstreams: 2 SUN-THESIS-2018.pdf: 1089905 bytes, checksum: 3d9b9576a5c750f5af2f31175c79a39b (MD5) LICENSE.txt: 4208 bytes, checksum: b1994d76056a00b2baba60aceee21327 (MD5) Previous issue date: 2018-04-23","Embargo set by: Seth Robbins for item 107445 Lift date: 2020-09-04T20:47:38Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Embargo set by: Seth Robbins for item 107445 Lift date: 2020-09-04T20:50:11Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system","Limited Restriction Lifted for Item 107445 on 2020-09-05T09:15:20Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Long-term n fertilization affects soil bacterial communities in agronomic fields"]}]}],"canonical_facts":{"dc:contributor":["Villamil, Maria Bonita"],"dc:creator":["Sun, Renpeng"],"dc:date":["2018-04-23","2018-09-04T20:47:27Z","2020-09-05T09:15:20Z","2018-05"],"dc:description":["Soil microbial communities influence plant productivity, chemistry and structure of soil and nutrient cycling. In turn, long-term nitrogen fertilization changes soil physical and chemical properties and thus influences soil microbiomes. In-depth understanding of the long-term nitrogen fertilization effects on soil microbial communities is critical for improving fertilization practices to optimize the microbiomes for soil health, productivity and sustainability. Our goal was to characterize the microbial community structure under long-term N gradient treatment in continuous corn (CCC) and to investigate the response of different soil microbial groups involved in the N cycle to different N fertilization levels. Field trial in CCC on three N fertilization rates (0, 210, and 280 kg N/ha) is arranged in a randomized complete block design (RCBD) with three replications. Using 16S rRNA gene-based pyrosequencing analysis of the V4 region and DNA extracted from soil in a 33-year-old agricultural field trial, we characterized the structure, composition and nitrogen cycling function of the bacterial communities involved. The downstream bioinformatics processing and analysis were conducted with QIIME (Quantitative Insights Into Microbial Ecology). V4 region of 16S rDNA gene sequences were clustered into operational taxonomic units (OTUs) and the bacterial community composition and diversity were analyzed based on the OTUs extracted. We applied principal component analyses (PCA) and canonical discriminant analysis (CDA) to our major bacterial phyla dataset. Our results indicate that high N fertilization level tended to decrease the diversity and richness of soil bacterial community and altered the relative abundance of the major bacterial phyla. There was a significant response of different soil bacterial groups involved in the N cycle to different N fertilization levels.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-05-01","The student, Renpeng Sun, accepted the attached license on 2018-04-19 at 14:20.","The student, Renpeng Sun, submitted this Thesis for approval on 2018-04-19 at 14:25.","This Thesis was approved for publication on 2018-04-23 at 09:49.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12384 on 2018-08-31 at 17:30:11","Made available in DSpace on 2018-09-04T20:47:27Z (GMT). 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