{"id":{"repo_id":"uic","oai_identifier":"oai:figshare.com:article/32993981"},"canonical_url":"https://search.dev.ndltd.org/etd/uic/oai:figshare.com:article/32993981","repository":{"repo_id":"uic","name":"University of Illinois - Chicago","base_url":"https://api.figshare.com/v2/oai"},"display":{"title":"NG2/CSPG4 Regulates Alveolar Bone and PDL Microstructure in a Sex-Dependent Manner","abstract":"Periodontal disease leads to alveolar bone loss through a complex, multifactorial process involving chronic inflammation. Understanding the molecular mechanisms that regulate bone formation and resorption in alveolar tissues is essential for identifying new therapeutic targets for periodontitis. Recent studies have shown that loss of type VI collagen reduces alveolar bone levels (Komori et. al, 2022). NG2/CSPG4, a type I transmembrane proteoglycan that binds to type VI collagen, is expressed in mineralizing tissues such as growth plate cartilage and cranial sutures. However, its role in regulating alveolar bone density and organization remains unclear. The objective of this study was to define the role of NG2/CSPG4 in the growth and development of alveolar bone and the periodontal ligament (PDL). To characterize alveolar bone in vivo, skulls from 16-week old male and female, control and NG2/CSPG4 knockout mice were collected, fixed overnight in 4% paraformaldehyde, and μCT scanned at 70 kV at a resolution of 12 μm voxel size. A region of interest (ROI) was contoured that included the alveolar bone measuring 140 slices centered between the roots of the first molar, and the same was repeated, measuring the PDL space with an ROI centered in the middle third of the mesial root of the first mandibular molar. The ROI was analyzed using the Trabecular Analysis package (Scanco Medical). Means comparing sex and genetic differences were compared using a one-way ANOVA with post-hoc Tukey corrections (α = 0.05). When comparing male and female mice, there was a sex dependent decrease in the ratio of bone volume to total volume and bone mineral density in both control and NG2ko mice (p<0.01), a sex dependent decrease in trabecular thickness in only the NG2ko mice (p<0.05; n=5), and a sex-dependent decrease in trabecular number in only control mice (p<0.01). When comparing control and NG2ko mice, there was an NG2-dependent decrease in trabecular number only in female mice (p < 0.0-5). Female NG2ko mice showed wider PDL widths compared to control mice (p<0.01). These findings demonstrate that NG2/CSPG4 contributes to alveolar bone and PDL microstructural homeostasis in female mice, suggesting its potential role as a novel therapeutic target and biomarker for bone resorption in periodontal disease.","abstract_html":"Periodontal disease leads to alveolar bone loss through a complex, multifactorial process involving chronic inflammation. Understanding the molecular mechanisms that regulate bone formation and resorption in alveolar tissues is essential for identifying new therapeutic targets for periodontitis. Recent studies have shown that loss of type VI collagen reduces alveolar bone levels (Komori et. al, 2022). NG2/CSPG4, a type I transmembrane proteoglycan that binds to type VI collagen, is expressed in mineralizing tissues such as growth plate cartilage and cranial sutures. However, its role in regulating alveolar bone density and organization remains unclear. The objective of this study was to define the role of NG2/CSPG4 in the growth and development of alveolar bone and the periodontal ligament (PDL). To characterize alveolar bone in vivo, skulls from 16-week old male and female, control and NG2/CSPG4 knockout mice were collected, fixed overnight in 4% paraformaldehyde, and μCT scanned at 70 kV at a resolution of 12 μm voxel size. A region of interest (ROI) was contoured that included the alveolar bone measuring 140 slices centered between the roots of the first molar, and the same was repeated, measuring the PDL space with an ROI centered in the middle third of the mesial root of the first mandibular molar. The ROI was analyzed using the Trabecular Analysis package (Scanco Medical). Means comparing sex and genetic differences were compared using a one-way ANOVA with post-hoc Tukey corrections (α = 0.05). When comparing male and female mice, there was a sex dependent decrease in the ratio of bone volume to total volume and bone mineral density in both control and NG2ko mice (p&lt;0.01), a sex dependent decrease in trabecular thickness in only the NG2ko mice (p&lt;0.05; n=5), and a sex-dependent decrease in trabecular number in only control mice (p&lt;0.01). When comparing control and NG2ko mice, there was an NG2-dependent decrease in trabecular number only in female mice (p &lt; 0.0-5). Female NG2ko mice showed wider PDL widths compared to control mice (p&lt;0.01). These findings demonstrate that NG2/CSPG4 contributes to alveolar bone and PDL microstructural homeostasis in female mice, suggesting its potential role as a novel therapeutic target and biomarker for bone resorption in periodontal disease.","abstract_has_math":false,"creators":["Polina Gubareva (6495170)"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2026,"date_issued":"2026-05-01T00:00:00Z","date_published":"2026-05-01T00:00:00Z","updated_at":"2026-07-27T21:33:41Z","subjects":["Murine Model"],"languages":[],"rights":["In Copyright"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://doi.org/10.25417/uic.32993981.v1","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Polina Gubareva (6495170)"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2026-05-01T00:00:00Z"]},{"key":"dc:relation","label":"Dc Relation","values":["https://figshare.com/articles/thesis/NG2_CSPG4_Regulates_Alveolar_Bone_and_PDL_Microstructure_in_a_Sex-Dependent_Manner/32993981"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Murine Model"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.25417/uic.32993981.v1"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Periodontal disease leads to alveolar bone loss through a complex, multifactorial process involving chronic inflammation. Understanding the molecular mechanisms that regulate bone formation and resorption in alveolar tissues is essential for identifying new therapeutic targets for periodontitis. Recent studies have shown that loss of type VI collagen reduces alveolar bone levels (Komori et. al, 2022). NG2/CSPG4, a type I transmembrane proteoglycan that binds to type VI collagen, is expressed in mineralizing tissues such as growth plate cartilage and cranial sutures. However, its role in regulating alveolar bone density and organization remains unclear. The objective of this study was to define the role of NG2/CSPG4 in the growth and development of alveolar bone and the periodontal ligament (PDL). To characterize alveolar bone in vivo, skulls from 16-week old male and female, control and NG2/CSPG4 knockout mice were collected, fixed overnight in 4% paraformaldehyde, and μCT scanned at 70 kV at a resolution of 12 μm voxel size. A region of interest (ROI) was contoured that included the alveolar bone measuring 140 slices centered between the roots of the first molar, and the same was repeated, measuring the PDL space with an ROI centered in the middle third of the mesial root of the first mandibular molar. The ROI was analyzed using the Trabecular Analysis package (Scanco Medical). Means comparing sex and genetic differences were compared using a one-way ANOVA with post-hoc Tukey corrections (α = 0.05). When comparing male and female mice, there was a sex dependent decrease in the ratio of bone volume to total volume and bone mineral density in both control and NG2ko mice (p<0.01), a sex dependent decrease in trabecular thickness in only the NG2ko mice (p<0.05; n=5), and a sex-dependent decrease in trabecular number in only control mice (p<0.01). When comparing control and NG2ko mice, there was an NG2-dependent decrease in trabecular number only in female mice (p < 0.0-5). Female NG2ko mice showed wider PDL widths compared to control mice (p<0.01). These findings demonstrate that NG2/CSPG4 contributes to alveolar bone and PDL microstructural homeostasis in female mice, suggesting its potential role as a novel therapeutic target and biomarker for bone resorption in periodontal disease."]},{"key":"dc:title","label":"Title","values":["NG2/CSPG4 Regulates Alveolar Bone and PDL Microstructure in a Sex-Dependent Manner"]}]}],"canonical_facts":{"dc:creator":["Polina Gubareva (6495170)"],"dc:date":["2026-05-01T00:00:00Z"],"dc:description":["Periodontal disease leads to alveolar bone loss through a complex, multifactorial process involving chronic inflammation. Understanding the molecular mechanisms that regulate bone formation and resorption in alveolar tissues is essential for identifying new therapeutic targets for periodontitis. Recent studies have shown that loss of type VI collagen reduces alveolar bone levels (Komori et. al, 2022). NG2/CSPG4, a type I transmembrane proteoglycan that binds to type VI collagen, is expressed in mineralizing tissues such as growth plate cartilage and cranial sutures. However, its role in regulating alveolar bone density and organization remains unclear. The objective of this study was to define the role of NG2/CSPG4 in the growth and development of alveolar bone and the periodontal ligament (PDL). To characterize alveolar bone in vivo, skulls from 16-week old male and female, control and NG2/CSPG4 knockout mice were collected, fixed overnight in 4% paraformaldehyde, and μCT scanned at 70 kV at a resolution of 12 μm voxel size. A region of interest (ROI) was contoured that included the alveolar bone measuring 140 slices centered between the roots of the first molar, and the same was repeated, measuring the PDL space with an ROI centered in the middle third of the mesial root of the first mandibular molar. The ROI was analyzed using the Trabecular Analysis package (Scanco Medical). Means comparing sex and genetic differences were compared using a one-way ANOVA with post-hoc Tukey corrections (α = 0.05). When comparing male and female mice, there was a sex dependent decrease in the ratio of bone volume to total volume and bone mineral density in both control and NG2ko mice (p<0.01), a sex dependent decrease in trabecular thickness in only the NG2ko mice (p<0.05; n=5), and a sex-dependent decrease in trabecular number in only control mice (p<0.01). When comparing control and NG2ko mice, there was an NG2-dependent decrease in trabecular number only in female mice (p < 0.0-5). Female NG2ko mice showed wider PDL widths compared to control mice (p<0.01). These findings demonstrate that NG2/CSPG4 contributes to alveolar bone and PDL microstructural homeostasis in female mice, suggesting its potential role as a novel therapeutic target and biomarker for bone resorption in periodontal disease."],"dc:identifier":["10.25417/uic.32993981.v1"],"dc:relation":["https://figshare.com/articles/thesis/NG2_CSPG4_Regulates_Alveolar_Bone_and_PDL_Microstructure_in_a_Sex-Dependent_Manner/32993981"],"dc:rights":["In Copyright"],"dc:subject":["Murine Model"],"dc:title":["NG2/CSPG4 Regulates Alveolar Bone and PDL Microstructure in a Sex-Dependent Manner"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T21:33:41Z"}