{"id":{"repo_id":"uic","oai_identifier":"oai:figshare.com:article/31451080"},"canonical_url":"https://search.dev.ndltd.org/etd/uic/oai:figshare.com:article/31451080","repository":{"repo_id":"uic","name":"University of Illinois - Chicago","base_url":"https://api.figshare.com/v2/oai"},"display":{"title":"Comparative Efficacy of MUS System vs Er:YAG Laser System in Reducing Bacterial Biofilms in Root Canals","abstract":"This in vitro study aimed to evaluate the efficacy of biofilm removal using two advanced irrigation systems: the Multisonic Ultracleaning System (MUS) GentleWave® with the CleanFlow™ handpiece, and the erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (LightWalker® Fotona) for Laser-Activated Irrigation (LAI). The modes used in LAI included Photon Induced Photo-acoustic Streaming (PIPS) and Shock Wave Enhanced Emission Photoacoustic Streaming (SWEEPS). To conduct this evaluation, forty extracted human mandibular molars meeting the inclusion criteria were collected, processed, and sterilized following Institutional Review Board (IRB) approval. The teeth were inoculated with Enterococcus faecalis and cultured for 21 days to develop biofilms. Biofilm formation in the canals was sampled and confirmed with confocal laser microscopy. After incubation, the samples were randomly divided into four groups (n=10): Standard Needle Irrigation (SNI), MUS system, Er:YAG SWEEPS, and Er:YAG PIPS. The teeth were instrumented to size 25/V06 and irrigated according to the respective treatment protocols. Bacterial samples were collected pre- and post-instrumentation. Data were analyzed using an analysis of variance (ANOVA) with a significance level of P < .05, followed by Dunnett’s and Tukey's tests. The results demonstrated that Er:YAG SWEEPS reduced E. faecalis significantly more than SNI and MUS systems (p < 0.05). However, there was no statistically significant difference in bacterial reduction when comparing MUS and PIPS (p > 0.05).These findings suggest that enhanced irrigation techniques might improve antimicrobial action against E. faecalis biofilms. The Er:YAG SWEEPS proved to be more effective than Er:YAG PIPS and MUS system within the limitations of the current study.","abstract_html":"This in vitro study aimed to evaluate the efficacy of biofilm removal using two advanced irrigation systems: the Multisonic Ultracleaning System (MUS) GentleWave® with the CleanFlow™ handpiece, and the erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (LightWalker® Fotona) for Laser-Activated Irrigation (LAI). The modes used in LAI included Photon Induced Photo-acoustic Streaming (PIPS) and Shock Wave Enhanced Emission Photoacoustic Streaming (SWEEPS). To conduct this evaluation, forty extracted human mandibular molars meeting the inclusion criteria were collected, processed, and sterilized following Institutional Review Board (IRB) approval. The teeth were inoculated with Enterococcus faecalis and cultured for 21 days to develop biofilms. Biofilm formation in the canals was sampled and confirmed with confocal laser microscopy. After incubation, the samples were randomly divided into four groups (n=10): Standard Needle Irrigation (SNI), MUS system, Er:YAG SWEEPS, and Er:YAG PIPS. The teeth were instrumented to size 25/V06 and irrigated according to the respective treatment protocols. Bacterial samples were collected pre- and post-instrumentation. Data were analyzed using an analysis of variance (ANOVA) with a significance level of P &lt; .05, followed by Dunnett’s and Tukey&#x27;s tests. The results demonstrated that Er:YAG SWEEPS reduced E. faecalis significantly more than SNI and MUS systems (p &lt; 0.05). However, there was no statistically significant difference in bacterial reduction when comparing MUS and PIPS (p &gt; 0.05).These findings suggest that enhanced irrigation techniques might improve antimicrobial action against E. faecalis biofilms. The Er:YAG SWEEPS proved to be more effective than Er:YAG PIPS and MUS system within the limitations of the current study.","abstract_has_math":false,"creators":["Malik Al-Grouz (21409517)"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-12-01T00:00:00Z","date_published":"2025-12-01T00:00:00Z","updated_at":"2026-07-27T21:34:21Z","subjects":["Laser Assisted Irrigation using Er:YAG Laser System","Mutisonic Ultracleaning System (MUS) GentleWave® System"],"languages":[],"rights":["In Copyright"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://doi.org/10.25417/uic.31451080.v1","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Malik Al-Grouz (21409517)"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-12-01T00:00:00Z"]},{"key":"dc:relation","label":"Dc Relation","values":["https://figshare.com/articles/thesis/Comparative_Efficacy_of_MUS_System_vs_Er_YAG_Laser_System_in_Reducing_Bacterial_Biofilms_in_Root_Canals/31451080"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Laser Assisted Irrigation using Er:YAG Laser System","Mutisonic Ultracleaning System (MUS) GentleWave® System"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.25417/uic.31451080.v1"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This in vitro study aimed to evaluate the efficacy of biofilm removal using two advanced irrigation systems: the Multisonic Ultracleaning System (MUS) GentleWave® with the CleanFlow™ handpiece, and the erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (LightWalker® Fotona) for Laser-Activated Irrigation (LAI). The modes used in LAI included Photon Induced Photo-acoustic Streaming (PIPS) and Shock Wave Enhanced Emission Photoacoustic Streaming (SWEEPS). To conduct this evaluation, forty extracted human mandibular molars meeting the inclusion criteria were collected, processed, and sterilized following Institutional Review Board (IRB) approval. The teeth were inoculated with Enterococcus faecalis and cultured for 21 days to develop biofilms. Biofilm formation in the canals was sampled and confirmed with confocal laser microscopy. After incubation, the samples were randomly divided into four groups (n=10): Standard Needle Irrigation (SNI), MUS system, Er:YAG SWEEPS, and Er:YAG PIPS. The teeth were instrumented to size 25/V06 and irrigated according to the respective treatment protocols. Bacterial samples were collected pre- and post-instrumentation. Data were analyzed using an analysis of variance (ANOVA) with a significance level of P < .05, followed by Dunnett’s and Tukey's tests. The results demonstrated that Er:YAG SWEEPS reduced E. faecalis significantly more than SNI and MUS systems (p < 0.05). However, there was no statistically significant difference in bacterial reduction when comparing MUS and PIPS (p > 0.05).These findings suggest that enhanced irrigation techniques might improve antimicrobial action against E. faecalis biofilms. The Er:YAG SWEEPS proved to be more effective than Er:YAG PIPS and MUS system within the limitations of the current study."]},{"key":"dc:title","label":"Title","values":["Comparative Efficacy of MUS System vs Er:YAG Laser System in Reducing Bacterial Biofilms in Root Canals"]}]}],"canonical_facts":{"dc:creator":["Malik Al-Grouz (21409517)"],"dc:date":["2025-12-01T00:00:00Z"],"dc:description":["This in vitro study aimed to evaluate the efficacy of biofilm removal using two advanced irrigation systems: the Multisonic Ultracleaning System (MUS) GentleWave® with the CleanFlow™ handpiece, and the erbium-doped yttrium-aluminum-garnet (Er:YAG) laser (LightWalker® Fotona) for Laser-Activated Irrigation (LAI). The modes used in LAI included Photon Induced Photo-acoustic Streaming (PIPS) and Shock Wave Enhanced Emission Photoacoustic Streaming (SWEEPS). To conduct this evaluation, forty extracted human mandibular molars meeting the inclusion criteria were collected, processed, and sterilized following Institutional Review Board (IRB) approval. The teeth were inoculated with Enterococcus faecalis and cultured for 21 days to develop biofilms. Biofilm formation in the canals was sampled and confirmed with confocal laser microscopy. After incubation, the samples were randomly divided into four groups (n=10): Standard Needle Irrigation (SNI), MUS system, Er:YAG SWEEPS, and Er:YAG PIPS. The teeth were instrumented to size 25/V06 and irrigated according to the respective treatment protocols. Bacterial samples were collected pre- and post-instrumentation. Data were analyzed using an analysis of variance (ANOVA) with a significance level of P < .05, followed by Dunnett’s and Tukey's tests. The results demonstrated that Er:YAG SWEEPS reduced E. faecalis significantly more than SNI and MUS systems (p < 0.05). However, there was no statistically significant difference in bacterial reduction when comparing MUS and PIPS (p > 0.05).These findings suggest that enhanced irrigation techniques might improve antimicrobial action against E. faecalis biofilms. The Er:YAG SWEEPS proved to be more effective than Er:YAG PIPS and MUS system within the limitations of the current study."],"dc:identifier":["10.25417/uic.31451080.v1"],"dc:relation":["https://figshare.com/articles/thesis/Comparative_Efficacy_of_MUS_System_vs_Er_YAG_Laser_System_in_Reducing_Bacterial_Biofilms_in_Root_Canals/31451080"],"dc:rights":["In Copyright"],"dc:subject":["Laser Assisted Irrigation using Er:YAG Laser System","Mutisonic Ultracleaning System (MUS) GentleWave® System"],"dc:title":["Comparative Efficacy of MUS System vs Er:YAG Laser System in Reducing Bacterial Biofilms in Root Canals"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T21:34:21Z"}