{"id":{"repo_id":"uic","oai_identifier":"oai:figshare.com:article/30425056"},"canonical_url":"https://search.dev.ndltd.org/etd/uic/oai:figshare.com:article/30425056","repository":{"repo_id":"uic","name":"University of Illinois - Chicago","base_url":"https://api.figshare.com/v2/oai"},"display":{"title":"Identifying Diagnostic Biomarkers: Gene Expression Profiling Leptin in Dental Pulp Tissue","abstract":"Recent studies emphasized the role of leptin/leptin receptor (LEP/LEPR) in normal and inflammatory conditions of dental pulp tissues. LEP/LEPR activity is linked to potential immunomodulation responses during bacterial-induced inflammatory conditions. Additionally, there is substantial association of LEP in dental pulp stem cells (DPSCs), odontoblast differentiation/function and mineralization processes. The objective of this study is to investigate differential leptin gene expression in hypoxic (inflammatory) conditions. DPSCs were isolated from healthy adult third molars, supplemented with 1% serum containing media or osteogenic media, and cultured in hypoxic (3%) or normoxic (21%) conditions for 21-days. Later, DPSCs collected were extracted for mRNA isolation. The mRNA was sequenced using NextSeq Illumina and was analyzed using the ERGO transcription tool DESeq2 (P<0.05) statistical method to determine differences in transcription and subsequent translation between groups. The count data was imported into R-package for analysis and sequence mapping to the appropriate gene. The genes and pathways were identified in KEGG pathways overlaying expression values. Over-expressed (p<0.05) genes include ACSL1, LEP, LEPR and PRKAA2 and under-expressed (p<0.05) genes include MAPK8, NFKBIE, RELA. DPSCs in hypoxic conditions shows down-regulation of AMPK signaling, which is related to cellular energy, transcription factors p65, NF-kappa-B and MAP Kinases associated with transcription regulation and differentiation. Up regulation of LEP/LEPR is associated with fat metabolism and both immune and inflammatory responses. Therefore, LEP signaling is an important regulator of DPSCs function. Furthermore, deciphering LEP/MAPK/Insulin signaling pathways in inflammatory condition of dental pulp could lead to new and more effective treatments for pulpitis and apical periodontitis.","abstract_html":"Recent studies emphasized the role of leptin/leptin receptor (LEP/LEPR) in normal and inflammatory conditions of dental pulp tissues. LEP/LEPR activity is linked to potential immunomodulation responses during bacterial-induced inflammatory conditions. Additionally, there is substantial association of LEP in dental pulp stem cells (DPSCs), odontoblast differentiation/function and mineralization processes. The objective of this study is to investigate differential leptin gene expression in hypoxic (inflammatory) conditions. DPSCs were isolated from healthy adult third molars, supplemented with 1% serum containing media or osteogenic media, and cultured in hypoxic (3%) or normoxic (21%) conditions for 21-days. Later, DPSCs collected were extracted for mRNA isolation. The mRNA was sequenced using NextSeq Illumina and was analyzed using the ERGO transcription tool DESeq2 (P&lt;0.05) statistical method to determine differences in transcription and subsequent translation between groups. The count data was imported into R-package for analysis and sequence mapping to the appropriate gene. The genes and pathways were identified in KEGG pathways overlaying expression values. Over-expressed (p&lt;0.05) genes include ACSL1, LEP, LEPR and PRKAA2 and under-expressed (p&lt;0.05) genes include MAPK8, NFKBIE, RELA. DPSCs in hypoxic conditions shows down-regulation of AMPK signaling, which is related to cellular energy, transcription factors p65, NF-kappa-B and MAP Kinases associated with transcription regulation and differentiation. Up regulation of LEP/LEPR is associated with fat metabolism and both immune and inflammatory responses. Therefore, LEP signaling is an important regulator of DPSCs function. Furthermore, deciphering LEP/MAPK/Insulin signaling pathways in inflammatory condition of dental pulp could lead to new and more effective treatments for pulpitis and apical periodontitis.","abstract_has_math":false,"creators":["Christopher Greenwaldt (22481830)"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-08-01T00:00:00Z","date_published":"2025-08-01T00:00:00Z","updated_at":"2026-07-27T21:34:45Z","subjects":["Health Sciences","Dentistry"],"languages":[],"rights":["In Copyright"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://doi.org/10.25417/uic.30425056.v1","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Christopher Greenwaldt (22481830)"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-08-01T00:00:00Z"]},{"key":"dc:relation","label":"Dc Relation","values":["https://figshare.com/articles/thesis/Identifying_Diagnostic_Biomarkers_Gene_Expression_Profiling_Leptin_in_Dental_Pulp_Tissue/30425056"]},{"key":"dc:type","label":"Dc Type","values":["Text","Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences","Dentistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.25417/uic.30425056.v1"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Recent studies emphasized the role of leptin/leptin receptor (LEP/LEPR) in normal and inflammatory conditions of dental pulp tissues. LEP/LEPR activity is linked to potential immunomodulation responses during bacterial-induced inflammatory conditions. Additionally, there is substantial association of LEP in dental pulp stem cells (DPSCs), odontoblast differentiation/function and mineralization processes. The objective of this study is to investigate differential leptin gene expression in hypoxic (inflammatory) conditions. DPSCs were isolated from healthy adult third molars, supplemented with 1% serum containing media or osteogenic media, and cultured in hypoxic (3%) or normoxic (21%) conditions for 21-days. Later, DPSCs collected were extracted for mRNA isolation. The mRNA was sequenced using NextSeq Illumina and was analyzed using the ERGO transcription tool DESeq2 (P<0.05) statistical method to determine differences in transcription and subsequent translation between groups. The count data was imported into R-package for analysis and sequence mapping to the appropriate gene. The genes and pathways were identified in KEGG pathways overlaying expression values. Over-expressed (p<0.05) genes include ACSL1, LEP, LEPR and PRKAA2 and under-expressed (p<0.05) genes include MAPK8, NFKBIE, RELA. DPSCs in hypoxic conditions shows down-regulation of AMPK signaling, which is related to cellular energy, transcription factors p65, NF-kappa-B and MAP Kinases associated with transcription regulation and differentiation. Up regulation of LEP/LEPR is associated with fat metabolism and both immune and inflammatory responses. Therefore, LEP signaling is an important regulator of DPSCs function. Furthermore, deciphering LEP/MAPK/Insulin signaling pathways in inflammatory condition of dental pulp could lead to new and more effective treatments for pulpitis and apical periodontitis."]},{"key":"dc:title","label":"Title","values":["Identifying Diagnostic Biomarkers: Gene Expression Profiling Leptin in Dental Pulp Tissue"]}]}],"canonical_facts":{"dc:creator":["Christopher Greenwaldt (22481830)"],"dc:date":["2025-08-01T00:00:00Z"],"dc:description":["Recent studies emphasized the role of leptin/leptin receptor (LEP/LEPR) in normal and inflammatory conditions of dental pulp tissues. LEP/LEPR activity is linked to potential immunomodulation responses during bacterial-induced inflammatory conditions. Additionally, there is substantial association of LEP in dental pulp stem cells (DPSCs), odontoblast differentiation/function and mineralization processes. The objective of this study is to investigate differential leptin gene expression in hypoxic (inflammatory) conditions. DPSCs were isolated from healthy adult third molars, supplemented with 1% serum containing media or osteogenic media, and cultured in hypoxic (3%) or normoxic (21%) conditions for 21-days. Later, DPSCs collected were extracted for mRNA isolation. The mRNA was sequenced using NextSeq Illumina and was analyzed using the ERGO transcription tool DESeq2 (P<0.05) statistical method to determine differences in transcription and subsequent translation between groups. The count data was imported into R-package for analysis and sequence mapping to the appropriate gene. The genes and pathways were identified in KEGG pathways overlaying expression values. Over-expressed (p<0.05) genes include ACSL1, LEP, LEPR and PRKAA2 and under-expressed (p<0.05) genes include MAPK8, NFKBIE, RELA. DPSCs in hypoxic conditions shows down-regulation of AMPK signaling, which is related to cellular energy, transcription factors p65, NF-kappa-B and MAP Kinases associated with transcription regulation and differentiation. Up regulation of LEP/LEPR is associated with fat metabolism and both immune and inflammatory responses. Therefore, LEP signaling is an important regulator of DPSCs function. Furthermore, deciphering LEP/MAPK/Insulin signaling pathways in inflammatory condition of dental pulp could lead to new and more effective treatments for pulpitis and apical periodontitis."],"dc:identifier":["10.25417/uic.30425056.v1"],"dc:relation":["https://figshare.com/articles/thesis/Identifying_Diagnostic_Biomarkers_Gene_Expression_Profiling_Leptin_in_Dental_Pulp_Tissue/30425056"],"dc:rights":["In Copyright"],"dc:subject":["Health Sciences","Dentistry"],"dc:title":["Identifying Diagnostic Biomarkers: Gene Expression Profiling Leptin in Dental Pulp Tissue"],"dc:type":["Text","Thesis"]},"updated_at":"2026-07-27T21:34:45Z"}