{"id":{"repo_id":"uhi-uk","oai_identifier":"oai:pure.atira.dk:studenttheses/f1cdd473-8a17-4e5a-966b-aa561938e354"},"canonical_url":"https://search.dev.ndltd.org/etd/uhi-uk/oai:pure.atira.dk:studenttheses/f1cdd473-8a17-4e5a-966b-aa561938e354","repository":{"repo_id":"uhi-uk","name":"University of the Highlands and Islands","base_url":"https://pureadmin.uhi.ac.uk/ws/oai"},"display":{"title":"Multi-omic Approaches to Investigate the Inflammatory Response in Activated Macrophage Cells","abstract":"Inflammation is a protective response to infection or injury and an essential
process in the maintenance of tissue homeostasis. It constitutes a highly complex
and tightly regulated sequence of events in which macrophages play a central
role, by eliciting cellular pathways that produce inflammatory mediators to
neutralize and eliminate invading pathogens and repair tissues damaged by
trauma. The focus of this thesis was to define the global lipid and protein
responses of cultured murine macrophages (RAW 264.7 cells) subjected to
inflammatory stimuli. Cells were treated with immune modulators that either
modelled bacterial infection (Kdo2-Lipid A) or reflected the signals produced by
damaged or dying cells during clearance (ATP). In-depth proteomic analyses
identified significant metabolic reprogramming along with a promotion of
macrophage differentiation and polarisation, and lipidomic analysis identified
significant regulation of arachidonic acid and glycerophospholipid metabolism.
Furthermore, a novel stable isotope labelling methodology was developed using
deuterium oxide (2H2O) in conjunction with high resolution mass spectrometry for the simultaneous measurement of the rates of synthesis of individual lipids and proteins on a cellular-wide scale. The results revealed altered dynamics of
ribosomal proteins and triglycerides following macrophage activation. Ultimately
advanced multi-omic approaches can be utilised to investigate the molecular
pathways of macrophages and provide additional perspectives on the
mechanisms associated with inflammatory responses.","abstract_html":"Inflammation is a protective response to infection or injury and an essential<br/>process in the maintenance of tissue homeostasis. It constitutes a highly complex<br/>and tightly regulated sequence of events in which macrophages play a central<br/>role, by eliciting cellular pathways that produce inflammatory mediators to<br/>neutralize and eliminate invading pathogens and repair tissues damaged by<br/>trauma. The focus of this thesis was to define the global lipid and protein<br/>responses of cultured murine macrophages (RAW 264.7 cells) subjected to<br/>inflammatory stimuli. Cells were treated with immune modulators that either<br/>modelled bacterial infection (Kdo2-Lipid A) or reflected the signals produced by<br/>damaged or dying cells during clearance (ATP). In-depth proteomic analyses<br/>identified significant metabolic reprogramming along with a promotion of<br/>macrophage differentiation and polarisation, and lipidomic analysis identified<br/>significant regulation of arachidonic acid and glycerophospholipid metabolism.<br/>Furthermore, a novel stable isotope labelling methodology was developed using<br/>deuterium oxide (2H2O) in conjunction with high resolution mass spectrometry for the simultaneous measurement of the rates of synthesis of individual lipids and proteins on a cellular-wide scale. The results revealed altered dynamics of<br/>ribosomal proteins and triglycerides following macrophage activation. Ultimately<br/>advanced multi-omic approaches can be utilised to investigate the molecular<br/>pathways of macrophages and provide additional perspectives on the<br/>mechanisms associated with inflammatory responses.","abstract_has_math":false,"creators":["Brace, Nicole"],"institution":"University of the Highlands and Islands","degree_name":"Doctor of Philosophy (awarded by UHI)","degree_level":"Doctoral Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Mainland, Ingrid"],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-7-9","date_published":"2021-7-9","updated_at":"2026-07-24T05:12:07Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.atira.dk:studenttheses/f1cdd473-8a17-4e5a-966b-aa561938e354"],"render_values":[{"text":"oai:pure.atira.dk:studenttheses/f1cdd473-8a17-4e5a-966b-aa561938e354","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.uhi.ac.uk/en/studentTheses/f1cdd473-8a17-4e5a-966b-aa561938e354","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Mainland, Ingrid"]},{"key":"dc:creator","label":"Author","values":["Brace, Nicole"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-7-9"]},{"key":"dc:date.issued","label":"Date","values":["2021-7-9"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Division of Biomedical Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of the Highlands and Islands"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.uhi.ac.uk/en/studentTheses/f1cdd473-8a17-4e5a-966b-aa561938e354"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy (awarded by UHI)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.atira.dk:studenttheses/f1cdd473-8a17-4e5a-966b-aa561938e354","https://pure.uhi.ac.uk/en/studentTheses/f1cdd473-8a17-4e5a-966b-aa561938e354"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.uhi.ac.uk/files/23212430/Nicole_Brace_Thesis_.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Inflammation is a protective response to infection or injury and an essential
process in the maintenance of tissue homeostasis. It constitutes a highly complex
and tightly regulated sequence of events in which macrophages play a central
role, by eliciting cellular pathways that produce inflammatory mediators to
neutralize and eliminate invading pathogens and repair tissues damaged by
trauma. The focus of this thesis was to define the global lipid and protein
responses of cultured murine macrophages (RAW 264.7 cells) subjected to
inflammatory stimuli. Cells were treated with immune modulators that either
modelled bacterial infection (Kdo2-Lipid A) or reflected the signals produced by
damaged or dying cells during clearance (ATP). In-depth proteomic analyses
identified significant metabolic reprogramming along with a promotion of
macrophage differentiation and polarisation, and lipidomic analysis identified
significant regulation of arachidonic acid and glycerophospholipid metabolism.
Furthermore, a novel stable isotope labelling methodology was developed using
deuterium oxide (2H2O) in conjunction with high resolution mass spectrometry for the simultaneous measurement of the rates of synthesis of individual lipids and proteins on a cellular-wide scale. The results revealed altered dynamics of
ribosomal proteins and triglycerides following macrophage activation. Ultimately
advanced multi-omic approaches can be utilised to investigate the molecular
pathways of macrophages and provide additional perspectives on the
mechanisms associated with inflammatory responses."]},{"key":"dc:title","label":"Title","values":["Multi-omic Approaches to Investigate the Inflammatory Response in Activated Macrophage Cells"]}]}],"canonical_facts":{"dc:contributor.advisor":["Mainland, Ingrid"],"dc:creator":["Brace, Nicole"],"dc:date":["2021-7-9"],"dc:date.issued":["2021-7-9"],"dc:description.abstract":["Inflammation is a protective response to infection or injury and an essential
process in the maintenance of tissue homeostasis. It constitutes a highly complex
and tightly regulated sequence of events in which macrophages play a central
role, by eliciting cellular pathways that produce inflammatory mediators to
neutralize and eliminate invading pathogens and repair tissues damaged by
trauma. The focus of this thesis was to define the global lipid and protein
responses of cultured murine macrophages (RAW 264.7 cells) subjected to
inflammatory stimuli. Cells were treated with immune modulators that either
modelled bacterial infection (Kdo2-Lipid A) or reflected the signals produced by
damaged or dying cells during clearance (ATP). In-depth proteomic analyses
identified significant metabolic reprogramming along with a promotion of
macrophage differentiation and polarisation, and lipidomic analysis identified
significant regulation of arachidonic acid and glycerophospholipid metabolism.
Furthermore, a novel stable isotope labelling methodology was developed using
deuterium oxide (2H2O) in conjunction with high resolution mass spectrometry for the simultaneous measurement of the rates of synthesis of individual lipids and proteins on a cellular-wide scale. The results revealed altered dynamics of
ribosomal proteins and triglycerides following macrophage activation. Ultimately
advanced multi-omic approaches can be utilised to investigate the molecular
pathways of macrophages and provide additional perspectives on the
mechanisms associated with inflammatory responses."],"dc:identifier":["oai:pure.atira.dk:studenttheses/f1cdd473-8a17-4e5a-966b-aa561938e354","https://pure.uhi.ac.uk/en/studentTheses/f1cdd473-8a17-4e5a-966b-aa561938e354"],"dc:identifier.uri":["https://pure.uhi.ac.uk/files/23212430/Nicole_Brace_Thesis_.pdf"],"dc:language":["eng"],"dc:publisher.department":["Division of Biomedical Sciences"],"dc:publisher.institution":["University of the Highlands and Islands"],"dc:relation.isreferencedby":["https://pure.uhi.ac.uk/en/studentTheses/f1cdd473-8a17-4e5a-966b-aa561938e354"],"dc:title":["Multi-omic Approaches to Investigate the Inflammatory Response in Activated Macrophage Cells"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral Thesis"],"dc:type.qualificationname":["Doctor of Philosophy (awarded by UHI)"]},"updated_at":"2026-07-24T05:12:07Z"}