{"id":{"repo_id":"uhi-uk","oai_identifier":"oai:pure.atira.dk:studenttheses/89c81964-751a-4aac-beb2-f6ddf6425c11"},"canonical_url":"https://search.dev.ndltd.org/etd/uhi-uk/oai:pure.atira.dk:studenttheses/89c81964-751a-4aac-beb2-f6ddf6425c11","repository":{"repo_id":"uhi-uk","name":"University of the Highlands and Islands","base_url":"https://pureadmin.uhi.ac.uk/ws/oai"},"display":{"title":"Using wildlife to biomonitor for antimicrobial resistance in the Scottish environment","abstract":"Antimicrobial resistance (AMR) is a threat to global health. Surveillance of AMR<br/>bacteria in wildlife host is key to understanding and tracking their spread<br/>throughout our environment. We established baseline data on the prevalence of<br/>AMR E. coli in Scottish wild deer, utilising faecal samples gathered from across the country. Using both breakpoint plate (sample level) and published disk-diffusion (isolate level) methods, we found that resistance to tetracycline was the most prevalent phenotype, while resistance to other antimicrobial classes was generally low or not detectable. However, instances of clinical resistance to fluoroquinolones, carbapenems, 3rd gen. cephalosporins, and genes associates with extended spectrum β-lactamase resistance were detected. We also investigated the use of freshwater invertebrates as a biomonitoring tool. We utilised invertebrate samples to obtain reference data on the AMR of E. coli present in the freshwater invertebrate gut, across three distinct river catchments. Using the breakpoint plate method, no significant difference in the prevalence of tetracycline or cefpodoxime resistance was observed between the three catchments. However, isolate based investigation revealed a much broader range of resistance phenotypes in those catchments with greater levels of anthropogenic activity. In isolates from both wild deer and freshwater invertebrates, we observed a population-wide shift in the phenotypic resistance for some antimicrobials, indicating that the use of bespoke cut-off values for the determination of non-wild type isolates would be preferable to published values. Using statistical modelling, we found potential links between the AMR observed in the study populations and livestock, human leisure activities, companion animals and other wildlife.","abstract_html":"Antimicrobial resistance (AMR) is a threat to global health. Surveillance of AMR&lt;br/&gt;bacteria in wildlife host is key to understanding and tracking their spread&lt;br/&gt;throughout our environment. We established baseline data on the prevalence of&lt;br/&gt;AMR E. coli in Scottish wild deer, utilising faecal samples gathered from across the country. Using both breakpoint plate (sample level) and published disk-diffusion (isolate level) methods, we found that resistance to tetracycline was the most prevalent phenotype, while resistance to other antimicrobial classes was generally low or not detectable. However, instances of clinical resistance to fluoroquinolones, carbapenems, 3rd gen. cephalosporins, and genes associates with extended spectrum β-lactamase resistance were detected. We also investigated the use of freshwater invertebrates as a biomonitoring tool. We utilised invertebrate samples to obtain reference data on the AMR of E. coli present in the freshwater invertebrate gut, across three distinct river catchments. Using the breakpoint plate method, no significant difference in the prevalence of tetracycline or cefpodoxime resistance was observed between the three catchments. However, isolate based investigation revealed a much broader range of resistance phenotypes in those catchments with greater levels of anthropogenic activity. In isolates from both wild deer and freshwater invertebrates, we observed a population-wide shift in the phenotypic resistance for some antimicrobials, indicating that the use of bespoke cut-off values for the determination of non-wild type isolates would be preferable to published values. Using statistical modelling, we found potential links between the AMR observed in the study populations and livestock, human leisure activities, companion animals and other wildlife.","abstract_has_math":false,"creators":["Elsby, Derek"],"institution":"University of the Highlands and Islands","degree_name":"Doctor of Philosophy (awarded by UHI)","degree_level":"Doctoral Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Taggart, Mark","Boyd, Kenneth"],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-1-18","date_published":"2021-1-18","updated_at":"2026-07-24T05:12:07Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.atira.dk:studenttheses/89c81964-751a-4aac-beb2-f6ddf6425c11"],"render_values":[{"text":"oai:pure.atira.dk:studenttheses/89c81964-751a-4aac-beb2-f6ddf6425c11","href":null,"code":true}]}]},"links":{"outbound_url":"https://pure.uhi.ac.uk/en/studentTheses/89c81964-751a-4aac-beb2-f6ddf6425c11","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Taggart, Mark","Boyd, Kenneth"]},{"key":"dc:contributor.sponsor","label":"Sponsor","values":["ESF studentship","Scottish Funding Council"]},{"key":"dc:creator","label":"Author","values":["Elsby, Derek"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-1-18"]},{"key":"dc:date.issued","label":"Date","values":["2021-1-18"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Environmental Research Institute"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of the Highlands and Islands"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://pure.uhi.ac.uk/en/studentTheses/89c81964-751a-4aac-beb2-f6ddf6425c11"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Doctor of Philosophy (awarded by UHI)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:pure.atira.dk:studenttheses/89c81964-751a-4aac-beb2-f6ddf6425c11","https://pure.uhi.ac.uk/en/studentTheses/89c81964-751a-4aac-beb2-f6ddf6425c11"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://pure.uhi.ac.uk/files/16888486/Derek_Elsby.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Antimicrobial resistance (AMR) is a threat to global health. Surveillance of AMR<br/>bacteria in wildlife host is key to understanding and tracking their spread<br/>throughout our environment. We established baseline data on the prevalence of<br/>AMR E. coli in Scottish wild deer, utilising faecal samples gathered from across the country. Using both breakpoint plate (sample level) and published disk-diffusion (isolate level) methods, we found that resistance to tetracycline was the most prevalent phenotype, while resistance to other antimicrobial classes was generally low or not detectable. However, instances of clinical resistance to fluoroquinolones, carbapenems, 3rd gen. cephalosporins, and genes associates with extended spectrum β-lactamase resistance were detected. We also investigated the use of freshwater invertebrates as a biomonitoring tool. We utilised invertebrate samples to obtain reference data on the AMR of E. coli present in the freshwater invertebrate gut, across three distinct river catchments. Using the breakpoint plate method, no significant difference in the prevalence of tetracycline or cefpodoxime resistance was observed between the three catchments. However, isolate based investigation revealed a much broader range of resistance phenotypes in those catchments with greater levels of anthropogenic activity. In isolates from both wild deer and freshwater invertebrates, we observed a population-wide shift in the phenotypic resistance for some antimicrobials, indicating that the use of bespoke cut-off values for the determination of non-wild type isolates would be preferable to published values. Using statistical modelling, we found potential links between the AMR observed in the study populations and livestock, human leisure activities, companion animals and other wildlife."]},{"key":"dc:title","label":"Title","values":["Using wildlife to biomonitor for antimicrobial resistance in the Scottish environment"]}]}],"canonical_facts":{"dc:contributor.advisor":["Taggart, Mark","Boyd, Kenneth"],"dc:contributor.sponsor":["ESF studentship","Scottish Funding Council"],"dc:creator":["Elsby, Derek"],"dc:date":["2021-1-18"],"dc:date.issued":["2021-1-18"],"dc:description.abstract":["Antimicrobial resistance (AMR) is a threat to global health. Surveillance of AMR<br/>bacteria in wildlife host is key to understanding and tracking their spread<br/>throughout our environment. We established baseline data on the prevalence of<br/>AMR E. coli in Scottish wild deer, utilising faecal samples gathered from across the country. Using both breakpoint plate (sample level) and published disk-diffusion (isolate level) methods, we found that resistance to tetracycline was the most prevalent phenotype, while resistance to other antimicrobial classes was generally low or not detectable. However, instances of clinical resistance to fluoroquinolones, carbapenems, 3rd gen. cephalosporins, and genes associates with extended spectrum β-lactamase resistance were detected. We also investigated the use of freshwater invertebrates as a biomonitoring tool. We utilised invertebrate samples to obtain reference data on the AMR of E. coli present in the freshwater invertebrate gut, across three distinct river catchments. Using the breakpoint plate method, no significant difference in the prevalence of tetracycline or cefpodoxime resistance was observed between the three catchments. However, isolate based investigation revealed a much broader range of resistance phenotypes in those catchments with greater levels of anthropogenic activity. In isolates from both wild deer and freshwater invertebrates, we observed a population-wide shift in the phenotypic resistance for some antimicrobials, indicating that the use of bespoke cut-off values for the determination of non-wild type isolates would be preferable to published values. Using statistical modelling, we found potential links between the AMR observed in the study populations and livestock, human leisure activities, companion animals and other wildlife."],"dc:identifier":["oai:pure.atira.dk:studenttheses/89c81964-751a-4aac-beb2-f6ddf6425c11","https://pure.uhi.ac.uk/en/studentTheses/89c81964-751a-4aac-beb2-f6ddf6425c11"],"dc:identifier.uri":["https://pure.uhi.ac.uk/files/16888486/Derek_Elsby.pdf"],"dc:language":["eng"],"dc:publisher.department":["Environmental Research Institute"],"dc:publisher.institution":["University of the Highlands and Islands"],"dc:relation.isreferencedby":["https://pure.uhi.ac.uk/en/studentTheses/89c81964-751a-4aac-beb2-f6ddf6425c11"],"dc:title":["Using wildlife to biomonitor for antimicrobial resistance in the Scottish environment"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral Thesis"],"dc:type.qualificationname":["Doctor of Philosophy (awarded by UHI)"]},"updated_at":"2026-07-24T05:12:07Z"}