Opioid inhibition of the growth of the transplantable androgen-responsive (AR) Shionogi mouse mammary carcinoma (SC115)
type: CreativeWork
description: We investigated the effects of steroid hormones, growth factors, and anti-growth factor antibodies on the growth of cells in primary culture from the transplantable androgen-responsive(AR) Shionogi mouse mammary carcinoma (SC115). In addition, the effects of opioid agonists and antagonists on the growth of SC115tumor cells in primary culture were examined. The possible role of opioids in mediating the differential tumor growth rates of mice exposed to experimental housing groups was also examined.SC115 tumor cells were cultured at 5 x 104 cells/cm2 onto collagen-coated 96-well microtiter tissue culture plates. The cells were incubated in medium containing 5% fetal bovine serum(FBS), 2% dextran charcoal-treated FBS (DCTFBS), or seurm-free medium, with or without different concentrations of dihydrotestosterone (DHT), hydrocortisone (HC), 178-estradiol(E2), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), anti-bFGF antibody with either DHT or bFGF, or anti-EGF antibody with either E2 or EGF. After 48 h, some of the cultures were exposed to 10-14-10-7 M of either 8-endorphin (5-EP), which binds to the 8 opioid receptor, cyclazocine (CZ),which binds to the k receptor, or morphine sulfate (MS), which binds to the g receptor. These opioids were added daily for 3 d. In some experiments, 10-8 and 10-6 M of naloxone, an opioid receptor antagonist, were added with the opioids. On day 5, the cultures were terminated and cell numbers determined by the tetrazolium dye reduction assay. The results show that AR SC115 tumor cells in serum-containing medium were significantly stimulated to grow in a dose-dependent manner with DHT ranging from 3.5 x 10-1° - 3•5 x10-6 M and bFGF ranging from 1-100 ng/ml and in serum-free medium were significantly stimulated to grow by bFGF at all concentrations examined (1-500 ng/ml). HC ranging from 10-9-10-5M also significantly stimulated SC115 tumor cell growth. In contrast, E2 (10-9-10-6 M) and EGF (1-100 ng/ml) at all concentr…
abstract: We investigated the effects of steroid hormones, growth factors, and anti-growth factor antibodies on the growth of cells in primary culture from the transplantable androgen-responsive(AR) Shionogi mouse mammary carcinoma (SC115). In addition, the effects of opioid agonists and antagonists on the growth of SC115tumor cells in primary culture were examined. The possible role of opioids in mediating the differential tumor growth rates of mice exposed to experimental housing groups was also examined.SC115 tumor cells were cultured at 5 x 104 cells/cm2 onto collagen-coated 96-well microtiter tissue culture plates. The cells were incubated in medium containing 5% fetal bovine serum(FBS), 2% dextran charcoal-treated FBS (DCTFBS), or seurm-free medium, with or without different concentrations of dihydrotestosterone (DHT), hydrocortisone (HC), 178-estradiol(E2), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), anti-bFGF antibody with either DHT or bFGF, or anti-EGF antibody with either E2 or EGF. After 48 h, some of the cultures were exposed to 10-14-10-7 M of either 8-endorphin (5-EP), which binds to the 8 opioid receptor, cyclazocine (CZ),which binds to the k receptor, or morphine sulfate (MS), which binds to the g receptor. These opioids were added daily for 3 d. In some experiments, 10-8 and 10-6 M of naloxone, an opioid receptor antagonist, were added with the opioids. On day 5, the cultures were terminated and cell numbers determined by the tetrazolium dye reduction assay. The results show that AR SC115 tumor cells in serum-containing medium were significantly stimulated to grow in a dose-dependent manner with DHT ranging from 3.5 x 10-1° - 3•5 x10-6 M and bFGF ranging from 1-100 ng/ml and in serum-free medium were significantly stimulated to grow by bFGF at all concentrations examined (1-500 ng/ml). HC ranging from 10-9-10-5M also significantly stimulated SC115 tumor cell growth. In contrast, E2 (10-9-10-6 M) and EGF (1-100 ng/ml) at all concentr…
author: Jian, Yi
language: eng
date published: 1993
date modified: 2026-07-24T05:07:21Z
provider: University of British Columbia
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of British Columbia

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2429/2077