{"id":{"repo_id":"ubc","oai_identifier":"oai:circle.library.ubc.ca:2429/1960"},"canonical_url":"https://search.dev.ndltd.org/etd/ubc/oai:circle.library.ubc.ca:2429/1960","repository":{"repo_id":"ubc","name":"University of British Columbia","base_url":"http://circle.library.ubc.ca/oai/request"},"display":{"title":"A genetic and molecular analysis of the bli-4 locus of Caenorhabditis elegans, an essential gene encoding KEX2-like subtilisin-typw serine endoproteases","abstract":"This thesis reports a characterization of genetic mutations and molecular structure of the bli-4 (I) gene of Caenorhabditis elegans. The bli-4locus had been previously defined by a single recessive mutation, e937, which disrupts the structure of adult-stage cuticle causing the formation of fluid-filled separations of the cuticle layers, or blisters. A characterization of e937 and eleven additional mutations is reported. The mutations were grouped into three classes based on phenotype and complementation analysis: Class I, represented by the single allele causing blistering, e937;Class II, nine lethal mutations that arrest development at the end of embryogenesis and that fail to complement all other mutations; and Class 111,two larval lethal mutations, s90 and h754, that complement e937. The complementation pattern provides evidence that all of these mutations are allelic, and that bli-4 is a complex locus with an essential function late in embryogenesis. A region of chromosome I that includes the bli-4 locus was identified by aligning the C. elegans genetic and physical maps. The bli-4 coding region was identified by using cosmids as hybridization probes to detect chromosomal alterations in the DNA of bli-4 mutant strains. Two mutations of bli-4 are small rearrangements of the gene; e937, is a 3.5 kilo base (kb)deletion, and h1010 is an insertion of the 1.6 kb transposable element, Tc1.Three protein products that differ at the carboxyl end, are predicted from alternately spliced bli-4 cDNA clones. The predicted proteins were designated blisterin A, blisterin B and blisterin C according to the order that the variantly spliced 3' ends occur on the chromosome. Of the three bli-4cDNA clones characterized, only blisterin B includes an open reading frame beginning with an ATG start codon. The blisterin A and blisterin C open reading frames begin within blisterin B, and are likely to be incomplete. The predicted blisterin B gene product has a potential secretion signal peptide atits amino terminal. Blisterin C has a potential Tran membrane domain near its carboxyl terminal while blisterin A and B lack this domain. The blisterins share significant sequence identity with kex2-like serine endoproteases ,which are responsible for the cleavage of secreted proteins in yeast and mammals. This characterization of bli-4 provides the first evidence for an essential role of a KEX2-like gene in the development of a multi-cellular organism.","abstract_html":"This thesis reports a characterization of genetic mutations and molecular structure of the bli-4 (I) gene of Caenorhabditis elegans. The bli-4locus had been previously defined by a single recessive mutation, e937, which disrupts the structure of adult-stage cuticle causing the formation of fluid-filled separations of the cuticle layers, or blisters. A characterization of e937 and eleven additional mutations is reported. The mutations were grouped into three classes based on phenotype and complementation analysis: Class I, represented by the single allele causing blistering, e937;Class II, nine lethal mutations that arrest development at the end of embryogenesis and that fail to complement all other mutations; and Class 111,two larval lethal mutations, s90 and h754, that complement e937. The complementation pattern provides evidence that all of these mutations are allelic, and that bli-4 is a complex locus with an essential function late in embryogenesis. A region of chromosome I that includes the bli-4 locus was identified by aligning the C. elegans genetic and physical maps. The bli-4 coding region was identified by using cosmids as hybridization probes to detect chromosomal alterations in the DNA of bli-4 mutant strains. Two mutations of bli-4 are small rearrangements of the gene; e937, is a 3.5 kilo base (kb)deletion, and h1010 is an insertion of the 1.6 kb transposable element, Tc1.Three protein products that differ at the carboxyl end, are predicted from alternately spliced bli-4 cDNA clones. The predicted proteins were designated blisterin A, blisterin B and blisterin C according to the order that the variantly spliced 3' ends occur on the chromosome. Of the three bli-4cDNA clones characterized, only blisterin B includes an open reading frame beginning with an ATG start codon. The blisterin A and blisterin C open reading frames begin within blisterin B, and are likely to be incomplete. The predicted blisterin B gene product has a potential secretion signal peptide atits amino terminal. Blisterin C has a potential Tran membrane domain near its carboxyl terminal while blisterin A and B lack this domain. The blisterins share significant sequence identity with kex2-like serine endoproteases ,which are responsible for the cleavage of secreted proteins in yeast and mammals. This characterization of bli-4 provides the first evidence for an essential role of a KEX2-like gene in the development of a multi-cellular organism.","abstract_has_math":false,"creators":["Peters, Kenneth W."],"institution":"University of British Columbia","degree_name":"Doctor of Philosophy - PhD","degree_level":"doctoral","degree_discipline":"Genetics","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1992,"date_issued":"1992","date_published":"1992","updated_at":"2026-07-24T05:07:28Z","subjects":[],"languages":["eng"],"rights":["For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2429/1960","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Peters, Kenneth W."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1992"]},{"key":"dc:publisher","label":"Institution","values":["University of British Columbia"]},{"key":"dc:type","label":"Dc Type","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Genetics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy - PhD"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of British Columbia"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["For non-commercial purposes only, such as research, private study and education. 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The mutations were grouped into three classes based on phenotype and complementation analysis: Class I, represented by the single allele causing blistering, e937;Class II, nine lethal mutations that arrest development at the end of embryogenesis and that fail to complement all other mutations; and Class 111,two larval lethal mutations, s90 and h754, that complement e937. The complementation pattern provides evidence that all of these mutations are allelic, and that bli-4 is a complex locus with an essential function late in embryogenesis. A region of chromosome I that includes the bli-4 locus was identified by aligning the C. elegans genetic and physical maps. The bli-4 coding region was identified by using cosmids as hybridization probes to detect chromosomal alterations in the DNA of bli-4 mutant strains. Two mutations of bli-4 are small rearrangements of the gene; e937, is a 3.5 kilo base (kb)deletion, and h1010 is an insertion of the 1.6 kb transposable element, Tc1.Three protein products that differ at the carboxyl end, are predicted from alternately spliced bli-4 cDNA clones. The predicted proteins were designated blisterin A, blisterin B and blisterin C according to the order that the variantly spliced 3' ends occur on the chromosome. Of the three bli-4cDNA clones characterized, only blisterin B includes an open reading frame beginning with an ATG start codon. The blisterin A and blisterin C open reading frames begin within blisterin B, and are likely to be incomplete. The predicted blisterin B gene product has a potential secretion signal peptide atits amino terminal. Blisterin C has a potential Tran membrane domain near its carboxyl terminal while blisterin A and B lack this domain. The blisterins share significant sequence identity with kex2-like serine endoproteases ,which are responsible for the cleavage of secreted proteins in yeast and mammals. This characterization of bli-4 provides the first evidence for an essential role of a KEX2-like gene in the development of a multi-cellular organism."]},{"key":"dc:format","label":"Dc Format","values":["6655669","application/pdf"]},{"key":"dc:title","label":"Title","values":["A genetic and molecular analysis of the bli-4 locus of Caenorhabditis elegans, an essential gene encoding KEX2-like subtilisin-typw serine endoproteases"]}]}],"canonical_facts":{"dc:creator":["Peters, Kenneth W."],"dc:date":["1992"],"dc:description":["This thesis reports a characterization of genetic mutations and molecular structure of the bli-4 (I) gene of Caenorhabditis elegans. The bli-4locus had been previously defined by a single recessive mutation, e937, which disrupts the structure of adult-stage cuticle causing the formation of fluid-filled separations of the cuticle layers, or blisters. A characterization of e937 and eleven additional mutations is reported. The mutations were grouped into three classes based on phenotype and complementation analysis: Class I, represented by the single allele causing blistering, e937;Class II, nine lethal mutations that arrest development at the end of embryogenesis and that fail to complement all other mutations; and Class 111,two larval lethal mutations, s90 and h754, that complement e937. The complementation pattern provides evidence that all of these mutations are allelic, and that bli-4 is a complex locus with an essential function late in embryogenesis. A region of chromosome I that includes the bli-4 locus was identified by aligning the C. elegans genetic and physical maps. The bli-4 coding region was identified by using cosmids as hybridization probes to detect chromosomal alterations in the DNA of bli-4 mutant strains. Two mutations of bli-4 are small rearrangements of the gene; e937, is a 3.5 kilo base (kb)deletion, and h1010 is an insertion of the 1.6 kb transposable element, Tc1.Three protein products that differ at the carboxyl end, are predicted from alternately spliced bli-4 cDNA clones. The predicted proteins were designated blisterin A, blisterin B and blisterin C according to the order that the variantly spliced 3' ends occur on the chromosome. Of the three bli-4cDNA clones characterized, only blisterin B includes an open reading frame beginning with an ATG start codon. The blisterin A and blisterin C open reading frames begin within blisterin B, and are likely to be incomplete. The predicted blisterin B gene product has a potential secretion signal peptide atits amino terminal. Blisterin C has a potential Tran membrane domain near its carboxyl terminal while blisterin A and B lack this domain. The blisterins share significant sequence identity with kex2-like serine endoproteases ,which are responsible for the cleavage of secreted proteins in yeast and mammals. This characterization of bli-4 provides the first evidence for an essential role of a KEX2-like gene in the development of a multi-cellular organism."],"dc:format":["6655669","application/pdf"],"dc:identifier":["http://hdl.handle.net/2429/1960","http://circle.library.ubc.ca/bitstream/2429/1960/1/ubc_1993_spring_phd_peters_kenneth.pdf"],"dc:language":["eng"],"dc:publisher":["University of British Columbia"],"dc:rights":["For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use."],"dc:title":["A genetic and molecular analysis of the bli-4 locus of Caenorhabditis elegans, an essential gene encoding KEX2-like subtilisin-typw serine endoproteases"],"dc:type":["Text"],"thesis:degree_discipline":["Genetics"],"thesis:degree_level":["doctoral"],"thesis:degree_name":["Doctor of Philosophy - PhD"],"thesis:institution_name":["University of British Columbia"]},"updated_at":"2026-07-24T05:07:28Z"}